Results 21 to 30 of about 12,712 (251)

Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement

Frontiers in Bioengineering and Biotechnology, 2020
The Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)⋅tRNAPyl pair can be used to incorporate non-canonical amino acids (ncAAs) into proteins at installed amber stop codons.
Han-Kai Jiang, Han-Kai Jiang, Han-Kai Jiang, Man-Nee Lee, Jo-Chu Tsou, Kuan-Wen Chang, Hsueh-Wei Tseng, Kuang-Po Chen, Yaw-Kuen Li, Yane-Shih Wang, Yane-Shih Wang, Yane-Shih Wang   +11 more
doaj   +1 more source

Response and Adaptation of Escherichia coli to Suppression of the Amber Stop Codon [PDF]

ChemBioChem, 2014
AbstractSome extant organisms reassign the amber stop codon to a sense codon through evolution, and suppression of the amber codon with engineered tRNAs has been exploited to expand the genetic code for incorporating non‐canonical amino acids (ncAAs) in live systems. However, it is unclear how the host cells respond and adapt to such amber suppression.
Wang, Qian, Sun, Tingting, Xu, Jianfeng, Shen, Zhouxin, Briggs, Steven P, Zhou, Demin, Wang, Lei   +6 more
openaire   +4 more sources

A Spotlight on Viruses—Application of Click Chemistry to Visualize Virus-Cell Interactions

Molecules, 2019
The replication of a virus within its host cell involves numerous interactions between viral and cellular factors, which have to be tightly controlled in space and time.
Thorsten G. Müller, Volkan Sakin, Barbara Müller   +2 more
doaj   +1 more source

Suppression of amber codons in Caulobacter crescentus by the orthogonal Escherichia coli histidyl-tRNA synthetase/tRNAHis pair. [PDF]

PLoS ONE, 2013
While translational read-through of stop codons by suppressor tRNAs is common in many bacteria, archaea and eukaryotes, this phenomenon has not yet been observed in the α-proteobacterium Caulobacter crescentus.
Jae-hyeong Ko, Paula Montero Llopis, Jennifer Heinritz, Christine Jacobs-Wagner, Dieter Söll   +4 more
doaj   +1 more source

Protein Modification Employing Non-Canonical Amino Acids to Prepare SUMOylation Detecting Bioconjugates

Pharmaceutics, 2022
Protein modification with non-canonical amino acids (ncAAs) represents a useful technology to afford homogenous samples of bioconjugates with site-specific modification.
Alexander C. Williard, Hannah J. Switzer, Christina A. Howard, Rui Yin, Brent L. Russell, Ritwik Sanyal, Shaun Yu, Trinity M. Myers, Brian M. Flood, Oliver Kerscher, Douglas D. Young   +10 more
doaj   +1 more source

Antibody fragments functionalized with non-canonical amino acids preserving structure and functionality - A door opener for new biological and therapeutic applications

Heliyon, 2023
Functionalization of proteins by incorporating reactive non-canonical amino acids (ncAAs) has been widely applied for numerous biological and therapeutic applications.
Hana Hanaee-Ahvaz, Monika Cserjan-Puschmann, Florian Mayer, Christopher Tauer, Bernd Albrecht, Paul G. Furtmüller, Birgit Wiltschi, Rainer Hahn, Gerald Striedner   +8 more
doaj   +1 more source

Efficient suppression of the amber codon in E. coli in vitro translation system [PDF]

FEBS Letters, 2005
An mRNA encoding the esterase from Alicyclobacillus acidocaldarius with catalytically essential serine codon (ACG) replaced by an amber (UAG) codon was used to study the suppression in in vitro translation system. Suppression of UAG by tRNASer(CUA) was monitored by determination of the full‐length and active esterase.
Agafonov, Dmitry E., Huang, Yiwei, Grote, Michael, Sprinzl, Mathias   +3 more
openaire   +2 more sources

Pyrrolysyl-tRNA Synthetase with a Unique Architecture Enhances the Availability of Lysine Derivatives in Synthetic Genetic Codes

Molecules, 2018
Genetic code expansion has largely relied on two types of the tRNA—aminoacyl-tRNA synthetase pairs. One involves pyrrolysyl-tRNA synthetase (PylRS), which is used to incorporate various lysine derivatives into proteins.
Atsushi Yamaguchi, Fumie Iraha, Kazumasa Ohtake, Kensaku Sakamoto   +3 more
doaj   +1 more source

Evaluation and optimisation of unnatural amino acid incorporation and bioorthogonal bioconjugation for site-specific fluorescent labelling of proteins expressed in mammalian cells

Biochemistry and Biophysics Reports, 2019
Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules.
Leonhard Jakob, Alexander Gust, Dina Grohmann   +2 more
doaj   +1 more source

Improved ANAP incorporation and VCF analysis reveal details of P2X7 current facilitation and a limited conformational interplay between ATP binding and the intracellular ballast domain

eLife, 2023
The large intracellular C-terminus of the pro-inflammatory P2X7 ion channel receptor (P2X7R) is associated with diverse P2X7R-specific functions. Cryo-EM structures of the closed and ATP-bound open full-length P2X7R recently identified a membrane ...
Anna Durner, Ellis Durner, Annette Nicke
doaj   +1 more source