Results 11 to 20 of about 12,712 (251)
Frontiers in Molecular Biosciences, 2022 The investigation of protein structures, functions and interactions often requires modifications to adapt protein properties to the specific application.
Jeffrey L. Schloßhauer +7 more
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Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code. [PDF]
PLoS ONE, 2012 The suppression of amber stop codons with non-canonical amino acids (ncAAs) is used for the site-specific introduction of many unusual functions into proteins.
Sebastian Nehring +2 more
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mBio, 2022 The cone-shaped mature HIV-1 capsid is the main orchestrator of early viral replication. After cytosolic entry, it transports the viral replication complex along microtubules toward the nucleus.
Sandra Schifferdecker +7 more
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Epigenetic chromatin modification by amber suppression technology [PDF]
Current Opinion in Chemical Biology, 2018 The genetic incorporation of unnatural amino acids (UAAs) into proteins by amber suppression technology provides unique avenues to study protein structure, function and interactions both in vitro and in living cells and organisms. This approach has been particularly useful for studying mechanisms of epigenetic chromatin regulation, since these ...
Neumann, Heinz +3 more
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eLife, 2023 Tyrosine kinases and SH2 (phosphotyrosine recognition) domains have binding specificities that depend on the amino acid sequence surrounding the target (phospho)tyrosine residue.
Allyson Li +4 more
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Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids
Frontiers in Genetics, 2023 Once thought to be non-naturally occurring, D-amino acids (DAAs) have in recent years been revealed to play a wide range of physiological roles across the tree of life, including in human systems.
Han-Kai Jiang +15 more
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TRNA2Gln Su+2 mutants that increase amber suppression [PDF]
Journal of Bacteriology, 1981 We selected mutants of lambda pSu+2 which had an increased ability to suppress on Escherichia coli trp B9601 amber mutation on translationally stringent rpsL594 streptomycin-resistant ribosomes. tRNA2Gin Su+2 molecules produced from eight independent mutants were purified, and their ribonucleic acid sequences were determined.
D, Bradley, J V, Park, L, Soll
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Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides
Frontiers in Bioengineering and Biotechnology, 2020 Synthetic biology holds promise to revolutionize the life sciences and biomedicine via expansion of macromolecular diversity outside the natural chemical space.
Zhenling Cui +2 more
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Tetracycline-Regulated Suppression of Amber Codons in Mammalian Cells [PDF]
Molecular and Cellular Biology, 1998 As an approach to inducible suppression of nonsense mutations in mammalian cells, we described recently an amber suppression system in mammalian cells dependent on coexpression of Escherichia coli glutaminyl-tRNA synthetase (GlnRS) along with the E. coli glutamine-inserting amber suppressor tRNA.
H J, Park, U L, RajBhandary
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Synthetic and Systems Biotechnology, 2022 Amber suppression has been widely used to incorporate unnatural amino acids (UNAAs) with unique structures or functional side-chain groups into specific sites of the target protein, which expands the scope of protein-coding chemistry.
Jiaqi Hou +7 more
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