Results 131 to 140 of about 5,660 (180)

Evolution of Cyclic Amidohydrolases: A Highly Diversified Superfamily

Journal of Molecular Evolution, 2013
Dihydroorotases are universal proteins catalyzing the third step of pyrimidine biosynthesis. These zinc metalloenzymes belong to the superfamily of cyclic amidohydrolases, comprising also other enzymes that are involved in degradation of either purines (allantoinases), pyrimidines (dihydropyrimidinases) or hydantoins (hydantoinases).
Barba, Matthieu, Labedan, Bernard, Glansdorff, Nicolas   +2 more
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Catalytic Properties of Purified Recombinant Anandamide Amidohydrolase

Prostaglandins & Other Lipid Mediators, 1999
The major degradative pathway of anandamide, an endogenous ligand for cannabinoid receptors, is its enzymatic hydrolysis to arachidonic acid and ethanolamine.1The enzyme responsible for this reaction has been referred to as anandamide amidohydrolase23or fatty acid amide hydrolase.4‘N-Acylethanolamine amidohydrolase’ reported much earlier by Schmid and ...
Natsuo, Ueda, Kazuhisa, Katayama, Sravan Kumar, Goparaju, Yuko, Kurahashi, Kenji, Yamanaka, Hiroshi, Suzuki, Shozo, Yamamoto   +6 more
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Mechanism and thermodynamics of ligand binding to auxin amidohydrolase

Journal of Molecular Recognition, 2011
AbstractBrILL2 is catalytically the most efficient auxin amidohydrolase from Brassica rapa, playing a key role in auxin metabolism by catalyzing its release from amino acid conjugates. Auxins, with the most abundant representative indole‐acetic acid ([1H‐indol‐3‐yl]‐acetic acid, IAA), are a group of plant hormones that in very small concentrations ...
Simunovic, Mijo, Zagrovic, Bojan, Tomic, Sanja   +2 more
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Enzymological and Molecular Biological Studies on Anandamide Amidohydrolase

1999
Previously we suggested that anandamide amidohydrolase partially purified from porcine brain catalyzed the anandamide synthesis. The reversibility of the anandamide hydrolytic reaction was confirmed with a recombinant enzyme of rat liver. We also showed that the recombinant enzyme had a wide substrate specificity hydrolyzing primary amides and esters ...
Ueda N, Katayama K, Kurahashi Y, Suzuki M, Suzuki H, Yamamoto S, Katoh I, Di Marzo V, De Petrocellis L   +8 more
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Characterization of unexplored amidohydrolase enzyme—pterin deaminase

Applied Microbiology and Biotechnology, 2016
Pterin deaminase is an amidohydrolase enzyme hydrolyzing pteridines to form lumazine derivatives and ammonia. The enzyme captured the attention of scientists as early as 1959 and had been patented for its application as an anticancer agent. It is ubiquitously present in prokaryotes and has been reported in some eukaryotes such as honey bee, silkworm ...
Angayarkanni, Jayaraman, Murugesan, Thandeeswaran, Ulaganathan, Priyadarsini, Shanmugam, Sabarathinam, K A Ayub, Nawaz, Muthusamy, Palaniswamy   +5 more
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Anandamide amidohydrolase (fatty acid amide hydrolase)

Prostaglandins & Other Lipid Mediators, 2000
Anandamide (N-arachidonoylethanolamine) loses its cannabimimetic activity when it is hydrolyzed to arachidonic acid and ethanolamine by the catalysis of an enzyme referred to as anandamide amidohydrolase or fatty acid amide hydrolase. Cravatt's group and our group cloned cDNA of the enzyme from rat, human, mouse and pig, and the primary structures ...
N, Ueda, S, Yamamoto
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Structural and Catalytic Diversity within the Amidohydrolase Superfamily

Biochemistry, 2005
The amidohydrolase superfamily comprises a remarkable set of enzymes that catalyze the hydrolysis of a wide range of substrates bearing amide or ester functional groups at carbon and phosphorus centers. The most salient structural landmark for this family of hydrolytic enzymes is a mononuclear or binuclear metal center embedded within the confines of a
Clara M, Seibert, Frank M, Raushel
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Purification and Characterization of Allantoate Amidohydrolase fromBacillus fastidiosus

Archives of Biochemistry and Biophysics, 1995
Allantoate amidohydrolase from Bacillus fastidiosus was purified 170-fold to homogeneity as judged by isoelectric focusing and nondenaturing and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The molecular mass was estimated to be 128 kDa. The enzyme appeared to be a homodimer with a subunit molecular mass of 66 kDa.
Xu, Z.W., Windt, F.E. de, Drift, C. van der   +2 more
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Novel Inhibitors of Brain, Neuronal, and Basophilic Anandamide Amidohydrolase

Biochemical and Biophysical Research Communications, 1997
Mammalian brain as well as mouse neuroblastoma (N18TG2) and rat basophilic leukaemia (RBL) cells were previously shown to contain "anandamide amidohydrolase', a membrane-bound enzyme sensitive to serine and cysteine protease inhibitors and catalyzing the hydrolysis of the endogenous cannabimimetic metabolite, anandamide (arachidonoyl-ethanolamide ...
De Petrocellis L, Melck D, Ueda N, Maurelli S, Kurahashi Y, Yamamoto S, Marino G, Di Marzo V   +7 more
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Response of amidohydrolases in soils to chloroform fumigation

Journal of Plant Nutrition and Soil Science, 2002
An ongoing challenge in enzymology is the differentiation between the various pools of enzymes in soils, i.e. between accumulated (extracellular) enzymes and enzymes associated with the microbial biomass (intracellular enzymes). In order to solve this problem, 10 surface soils representing a wide range of physico-chemical properties and purified ...
Susanne Klose, M. Ali Tabatabai
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