Results 51 to 60 of about 10,587 (231)

Ureide Catabolism in Soybeans [PDF]

Plant Physiology, 1988
We demonstrate that allantoate is catabolized in soybean seedcoat extracts by an enzyme complex that has allantoate amidohydrolase and ureidoglycolate amidohydrolase activities. Soybean seedcoat extracts released (14)CO(2) from [ureido-(14)C]ureidoglycolate under conditions in which urease is not detectable.
Rodney G. Winkler, Dale G. Blevins, Douglas D. Randall   +2 more
openaire   +3 more sources

Characterization of the metallo-dependent amidohydrolases responsible for “auxiliary” leucinyl removal in the biosynthesis of 2,2′-bipyridine antibiotics

Synthetic and Systems Biotechnology, 2017
2,2′-Bipyridine (2,2′-BiPy) is an attractive core structure present in a number of biologically active natural products, including the structurally related antibiotics caerulomycins (CAEs) and collismycins (COLs).
Ming Chen, Bo Pang, Ya-nan Du, Yi-peng Zhang, Wen Liu   +4 more
doaj   +1 more source

Engineering of a chromogenic enzyme screening system based on an auxiliary indole‐3‐carboxylic acid monooxygenase

MicrobiologyOpen, 2019
Here, we present a proof‐of‐principle for a new high‐throughput functional screening of metagenomic libraries for the selection of enzymes with different activities, predetermined by the substrate being used. By this approach, a total of 21 enzyme‐coding
Vida Časaitė, Mikas Sadauskas, Justas Vaitekūnas, Renata Gasparavičiūtė, Rita Meškienė, Izabelė Skikaitė, Mantas Sakalauskas, Jevgenija Jakubovska, Daiva Tauraitė, Rolandas Meškys   +9 more
doaj   +1 more source

Probing the Active Site of Class 3 L-Asparaginase by Mutagenesis: Mutations of the Ser-Lys Tandems of ReAV

Biomolecules
The ReAV enzyme from Rhizobium etli, a representative of Class 3 L-asparaginases, is sequentially and structurally different from other known L-asparaginases. This distinctiveness makes ReAV a candidate for novel antileukemic therapies.
Kinga Pokrywka, Marta Grzechowiak, Joanna Sliwiak, Paulina Worsztynowicz, Joanna I. Loch, Milosz Ruszkowski, Miroslaw Gilski, Mariusz Jaskolski   +7 more
doaj   +1 more source

How innate immunity proteins kill bacteria and why they are not prone to resistance [PDF]

, 2017
Recent advances on antibacterial activity of peptidoglycan recognition proteins (PGRPs) offer some insight into how innate immunity has retained its antimicrobial effectiveness for millions of years with no frequent emergence of resistant strains. First,
Dziarski, Roman, Gupta, Dipika
core   +1 more source

SKN-1 regulates stress resistance downstream of amino catabolism pathways

iScience, 2022
Summary: The deleterious potential to generate oxidative stress is a fundamental challenge to metabolism. The oxidative stress response transcription factor, SKN-1/NRF2, can sense and respond to changes in metabolic state, although the mechanism and ...
Phillip A. Frankino, Talha F. Siddiqi, Theodore Bolas, Raz Bar-Ziv, Holly K. Gildea, Hanlin Zhang, Ryo Higuchi-Sanabria, Andrew Dillin   +7 more
doaj   +1 more source

Biochemical characterization of L-asparaginase isoforms from Rhizobium etli—the boosting effect of zinc

Frontiers in Chemistry
L-Asparaginases, divided into three structural Classes, catalyze the hydrolysis of L-asparagine to L-aspartic acid and ammonia. The members of Class 3, ReAIV and ReAV, encoded in the genome of the nitrogen fixing Rhizobium etli, have the same fold ...
Joanna Sliwiak, Paulina Worsztynowicz, Kinga Pokrywka, Joanna I. Loch, Marta Grzechowiak, Mariusz Jaskolski, Mariusz Jaskolski   +6 more
doaj   +1 more source

Diverse origins of microbial L-asparaginases and their current miscellaneous applications

Archives of Pharmaceutical Sciences Ain Shams University, 2019
L-asparaginase, also known as amidohydrolase, catalyzes the breakdown of asparagine into aspartic acid and ammonia. Due to its ability to inhibit the biosynthesis of protein lymphoblasts, it is used to treat acute lymphoblastic leukemia (ALL).
Nada A. Abdelrazek, Walid Faisal Elkhatib , Marwa M. Raafat, Mohammad Aboulwafa   +3 more
doaj   +1 more source

Immune-Mediated Inflammation May Contribute to the Pathogenesis of Cardiovascular Disease in Mucopolysaccharidosis Type I. [PDF]

, 2016
BackgroundCardiovascular disease, a progressive manifestation of α-L-iduronidase deficiency or mucopolysaccharidosis type I, continues in patients both untreated and treated with hematopoietic stem cell transplantation or intravenous enzyme replacement ...
Dickson, Patricia I, Ellinwood, N Matthew, Esko, Jeffrey D, Gordts, Philip L, Khalid, Omar, Schwartz, Philip H, Vera, Moin U, Wang, Raymond Y   +7 more
core   +3 more sources

Molecular cloning and characterization of an amidase from Arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid [PDF]

, 2003
Acylamidohydrolases from higher plants have not been characterized or cloned so far. AtAMI1 is the first member of this enzyme family from a higher plant and was identified in the genome of Arabidopsis thaliana based on sequence homology with the ...
Andersson, Ausubel, Bar, Barkan, Bartel, Bradford, Chou, Cravatt, Daniel Neu, Davies, Eckhardt, Elmar W Weiler, Hillebrand, Igoshi, Kawaguchi, Kawaguchi, Klee, Klee, Kobayashi, Kobayashi, Kobayashi, Laemmli, LeClere, Magie, Manulis, McKinney, Sambrook, Saotome, Scharf, Schägger, Stephan Pollmann, Takahashi, Towbin, Vorwerk, Weiler, Yamada, Östin   +36 more
core   +2 more sources