Results 71 to 80 of about 4,894 (170)

Biochemical properties of penicillin amidohydrolase from Micrococcus luteus [PDF]

Applied and Environmental Microbiology, 1979
Some biochemical properties of whole-cell penicillin amidohydrolase from Micrococcus luteus have been studied. This whole-cell enzyme showed its maximal activity at 36 degrees C at pH 7.5. It was found that the activation energy of this enzyme was 8.03 kcal (ca. 33.6 kJ) per mol, and this amidohydrolase showed first-order decay at 36 degrees C.
D H, Nam, D D, Ryu
openaire   +2 more sources

Crystal structure of carbon–nitrogen hydrolase from Helicobacter pylori G27

Acta Crystallographica Section F, Volume 82, Issue 3, Page 66-74, March 2026.
H. pylori carbon–nitrogen hydrolase was produced and crystallized, and its 2.1 Å resolution structure is reported.Carbon–nitrogen hydrolases (CNHs) are members of the diverse nitrilase superfamily of enzymes that facilitate cellular adaptation to environmental stress by metabolizing nitrogen, detoxifying xenobiotics and catabolizing environmentally ...
Aruesha Srivastava, Jesuferanmi P. Ayanlade, Lema Suleiman, Hannah Udell, Jan Abendroth, Donald J. Lorimer, Thomas E. Edwards, Bart L. Staker, Rachael Zigweid, Sandhya Subramanian, Peter J. Myler, Graham Chakafana, Oluwatoyin A. Asojo   +12 more
wiley   +1 more source

Transposon mutagenesis in a hyper-invasive clinical isolate of Campylobacter jejuni reveals a number of genes with potential roles in invasion [PDF]

, 2010
Transposon mutagenesis has been applied to a hyper-invasive clinical isolate of Campylobacter jejuni, 01/51. A random transposon mutant library was screened in an in vitro assay of invasion and 26 mutants with a significant reduction in invasion were ...
Bagnall, MC, Grant, AJ, Javed, MA, Manning, G, Maskell, DJ, Newell, DG   +5 more
core   +1 more source

Thermotolerant D‐Hydantoinases for Efficient and Stereoselective Intermediate Synthesis of (R)‐3‐Isobutylglutaric Acid Monoamide, a Key Intermediate in Pregabalin Production

Microbial Biotechnology, Volume 19, Issue 3, March 2026.
We engineered thermo‐alkali‐stable D‐hydantoinases via mutagenesis (M63A/F65H/C317T) to resolve Pregabalin's challenging chiral splitting, achieving ee value > 97% for key intermediate (R)‐3‐isobutylglutaric acid monoamide (R‐IBM) synthesis under extreme conditions (pH 10, 70°C–80°C).
Jiujiuzi Zhang, Bin Zhi, Zhenyu Xie, Beibei Zhang, Ning Wang, Wanpeng Wang   +5 more
wiley   +1 more source

Revisiting D‐Acylases for D‐Amino Acid Production

Microbial Biotechnology
N‐Acyl‐D‐amino acid deacylases (EC 3.5.1.81, also known as D‐acylases) have been studied for decades for their utility in the kinetic resolution of N‐acetyl‐D,L‐amino acids (NAAs) due to a marked stereospecificity. In conjunction with an N‐succinyl‐amino
Sergio Martínez‐Rodríguez, Jose Antonio Gavira   +1 more
doaj   +1 more source

Characterization of Jasmonoyl-Isoleucine (JA-Ile) Hormonal Catabolic Pathways in Rice upon Wounding and Salt Stress

Rice, 2019
Background Jasmonate (JA) signaling and functions have been established in rice development and response to a range of biotic or abiotic stress conditions.
Mohamed Hazman, Martin Sühnel, Sandra Schäfer, Julie Zumsteg, Agnès Lesot, Fréderic Beltran, Valentin Marquis, Laurence Herrgott, Laurence Miesch, Michael Riemann, Thierry Heitz   +10 more
doaj   +1 more source

Identification of a novel link connecting indole‐3‐acetamide with abscisic acid biosynthesis and signaling

New Phytologist, Volume 249, Issue 5, Page 2452-2466, March 2026.
Summary Plants orchestrate their developmental processes and responses to environmental stimuli through a sophisticated network of small signaling molecules, termed phytohormones. Among these, auxins are recognized for their role in promoting plant growth.
José Moya‐Cuevas, Paloma Ortiz‐García, Adrián González Ortega‐Villaizán, Irene Viguera‐Leza, Andrés Pérez‐González, Javier Paz‐Ares, Carlos Alonso‐Blanco, Jesús Vicente‐Carbajosa, Stephan Pollmann   +8 more
wiley   +1 more source

Subcellular localization and tissue specific expression of amidase 1 from Arabidopsis thaliana [PDF]

, 2006
Amidase 1 (AMI1) from Arabidopsis thaliana converts indole-3-acetamide (IAM), into indole-3-acetic acid (IAA). AMI1 is part of a small isogene family comprising seven members in A.
A Müller, A Müller, A Vojta, A Wachter, AM Metz, AW Woodward, BA Stelmach, BF Cravatt, Daniel Neu, DE Saslowsky, DK Giang, E Kemper, Elmar W. Weiler, EW Weiler, FA Ausubel, H Towbin, J Haseloff, J Sambrook, J-F Mayaux, JJ Finer, K Sohrt, KD Scharf, M Kawaguchi, M Kawaguchi, M Kobayashi, M Madera, M Saotome, MH Bracey, MM Bradford, MP Patricelli, NM Upadhyaya, NR Hofmann, O Chew, O Kleiner, Oliver Berkowitz, R Shrestha, R Shrestha, S Brenner, S Pollmann, S Pollmann, S Shin, S Shin, S Vorwerk, SR Cutler, Stephan Pollmann, T Murashige, Thomas Lehmann, Tina Schäfer, VS Reddy, WB Bruce, Y Arai, YJ Lee, YQ An, YS Kim   +53 more
core   +2 more sources

Effects of pH on the inhibition of fatty acid amidohydrolase by ibuprofen [PDF]

British Journal of Pharmacology, 2001
The pharmacological properties of fatty acid amidohydrolase (FAAH) at different assay pH values were investigated using [3H]‐anandamide ([3H]‐AEA) as substrate in rat brain homogenates and in COS‐7 cells transfected with wild type and mutant FAAH. Rat brain hydrolysis of [3H]‐AEA showed pH dependency with an optimum around pH 8‐9.
S, Holt, J, Nilsson, R, Omeir, G, Tiger, C J, Fowler   +4 more
openaire   +3 more sources

Functional Materials for Environmental Energy Harvesting in Smart Agriculture via Triboelectric Nanogenerators

Advanced Functional Materials, Volume 36, Issue 13, 12 February 2026.
This review explores functional and responsive materials for triboelectric nanogenerators (TENGs) in sustainable smart agriculture. It examines how particulate contamination and dirt affect charge transfer and efficiency. Environmental challenges and strategies to enhance durability and responsiveness are outlined, including active functional layers ...
Rafael R. A. Silva, Giulio Fatti, Emanuel Carlos, Guilherme Ferreira, Sumita Goswami, Suman Nandy, Luiz H. C. Mattoso, Elvira Fortunato, Caio G. Otoni, Rodrigo Martins   +9 more
wiley   +1 more source