Results 61 to 70 of about 21,738 (177)
, 2019 Supplemental Fig. 3 A and B, WB: Wt ZAG and its glycomutants were overexpressed in Expi293F cells and affinity purified using their GST-tags. ZAG (after GST removal) was incubated with and without recombinant AOC3, in the presence of H2O2 (A) or benzylamine (B).
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Equine Veterinary Journal, EarlyView.Abstract Background There is emerging evidence for the role of the immune system in osteoarthritis (OA) pathophysiology; however, little is known about how immune cells and the synovial transcriptome are altered in naturally occurring equine OA. Objectives To evaluate synovial fluid (SF) and synovial membrane (SM) immune cell populations and the SM ...
E. J. Secor +7 more
wiley +1 more source
Comparative study of the conformational lock, dissociative thermal inactivation and stability of Euphorbia latex and lentil seedling amine oxidases [PDF]
The thermal stability of copper/quinone containing amine oxidases from Euphorbia characias latex (ELAO) and lentil seedlings (LSAO) was measured in 100 mM potassium phosphate buffer (pH 7.0) following changes in absorbance at 292 nm. ELAO was shown to be
احمد, فیضان +7 more
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Rapidly self-deoxygenating controlled radical polymerization in water via in situ disproportionation of Cu(i) [PDF]
, 2020 Rapidly self-deoxygenating Cu-RDRP in aqueous media is investigated. The disproportionation of Cu(I)/Me6Tren in water towards Cu(II) and highly reactive Cu(0) leads to O2-free reaction environments within the first seconds of the reaction, even when the ...
Haddleton, David M. +4 more
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Recent Progress on Enzyme Immobilization: Materials, Strategies, and Applications
Food Bioengineering, EarlyView.Enzyme immobilization technology has undergone substantial evolution driven by advances in materials science. This review systematically examines the recent advances in enzyme immobilization since 2024, with a focused analysis of five major categories of enzyme carriers including covalent organic framework, metal‐organic frameworks, polymers, carbon ...
Shuran Wang +7 more
wiley +1 more source
A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase [PDF]
, 2015 A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ...
Baud, D +4 more
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Binuclear Copper‐Dependent Oxidative Enzymes Involved in Fungal Natural Product Modifications
Journal of the Chinese Chemical Society, EarlyView.This article summarizes recent biochemical characterizations of a new enzyme family named by the authors as binuclear copper‐dependent oxidative enzymes (BiNCOs). Found in fungal natural product biosynthesis, BiNCOs catalyze diverse CH functionalization reactions, including C(sp3)H halogenation, C(sp3)H hydroxylation, C(sp3)O macrocyclization, and ...
Chen‐Yu Chiang, Masao Ohashi, Yi Tang
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Computer-aided laccase engineering: toward biological oxidation of arylamines [PDF]
, 2016 Oxidation of arylamines, such as aniline, is of high industrial interest and laccases have been proposed as biocata-lysts to replace harsh chemical oxidants.
Acebes, Sandra +7 more
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A personal 360° view of applications of ‘biomimetic’ molecular recognition
Journal of Chemical Technology &Biotechnology, EarlyView.Abstract Molecular recognition between biological molecules has formed the basis for innumerable applications in biotechnology for the last seven decades or so. Techniques such as affinity chromatography, solid‐phase and aqueous two‐phase extraction, affinity precipitation, biomimetic catalytic systems, biosensors and molecular imprinting all exploit ...
Christopher R Lowe
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Conformational lock and thermal inactivation kinetics of Euphorbia amine oxidase [PDF]
The kinetics of thermal inactivation of copper-containing amine oxidase from euphorbia latex (ELAO) were studied in a 100-mM sodium phosphate buffer, pH 7, using cadavarine as the substrate.
امانی, مجتبی +6 more
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