Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Nitrogen compounds and enzyme activities in sorghum induced to water deficit during three stages
The aim of this study was to evaluate and explicate the changes in the nitrogen compounds and enzyme activities in Sorghum bicolor plants (cv. BR-700) submitted to water deficit during vegetative, reproductive and maturation stages.
C.F. Oliveira Neto +9 more
doaj +1 more source
Anaerobic ammonium oxidation for treatment of ammonium-rich wastewaters [PDF]
The concept of anaerobic ammonium oxidation (ANAMMOX) is presently of great interest. The functional bacteria belonging to the Planctomycete phylum and their metabolism are investigated by microbiologists. Meanwhile, the ANAMMOX is equally valuable in treatment of ammonium-rich wastewaters.
Lei, Zhang +3 more
openaire +2 more sources
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
Collection, preparation and analysis of NH4-1: a standard reference material for ammonium determination [PDF]
Bradley et al. (1990) discussed the need for a standard reference material for ammonium determinations. Subsequently, it was decided to collect a bulk sample and prepare this first as a BGS in-house standard; later, if resources permitted, full ...
Bradley, A.D.
core +3 more sources
Molybdenum supply increases 15N-nitrate uptake by maize
Nitrogen (N) is widely used in maize (Zea mays L.) production. The supply of molybdenum (Mo) can increase the recovery of N by the plant due to the role of this micronutrient in the assimilation of nitrate through nitrate reductase (NR).
Lílian A. Moreira +4 more
doaj +1 more source
Soil mineral nitrogen and the rating of CaCl2 extractable nutrients
It was assumed that the determination of the mineral nitrogen (Nmin) content in the 0.01 mol/L CaCl2 could rely on measurements of single form NO3--N, NH4+-N or both, and even including other extractable nutrients.
Remigiusz ŁUKOWIAK +2 more
doaj +1 more source
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
Plant invasions play a significant role in global environmental change. Traditionally, it was believed that invasive plants absorb and utilize nitrogen (N) more efficiently than native plants by adjusting their preferred N forms in accordance with the ...
Xingang Chang, Wenying Wang, Huakun Zhou
doaj +1 more source
Type I interferons modulate autophagy to shape gemcitabine response in pancreatic cancer cells
Type I interferons differentially modulate autophagy and the response of pancreatic cancer cells to gemcitabine. IFNα2b stimulates autophagic flux and protects cells from gemcitabine‐induced cell death, contributing to chemoresistance. In contrast, IFNβ1a inhibits autophagosome formation and enhances gemcitabine‐induced cell death, resulting in ...
Lucy E. Bonilla +10 more
wiley +1 more source

