Abstract Objective This study investigated the relationship between the consumption of ultra‐processed foods (UPF), dietary profile, and inflammation on the intestinal microbiome in children. Methods A cross‐sectional study was conducted using data from a community‐based controlled trial involving 82 children aged 7–11 years enrolled in public schools ...
Cristiane Cosmo Silva‐Luis +3 more
wiley +1 more source
The unresolved struggle of 16S rRNA amplicon sequencing: a benchmarking analysis of clustering and denoising methods. [PDF]
Fares M +7 more
europepmc +1 more source
Abstract BACKGROUND Bread wheat (Triticum aestivum L.) is a major global food crop, and understanding its maternal lineage and genetic diversity is essential for breeding, authentication, and evolutionary studies. Chloroplast genomes provide valuable markers for phylogenetic inference and cultivar discrimination; however, conventional plant DNA ...
Kang‐Rae Kim +6 more
wiley +1 more source
Benchmarking 16S rRNA Gene-Based Approaches to Bacterial Taxonomy Assignment Based on Amplicon Sequencing With Illumina and Oxford Nanopore. [PDF]
Hoffbeck C +3 more
europepmc +1 more source
Comparison of RNA- and DNA-based 16S amplicon sequencing to find the optimal approach for the analysis of the uterine microbiome. [PDF]
Dyroff AI +6 more
europepmc +1 more source
Computational Comparison of Differential Splicing Tools for Targeted RNA Long-Amplicon Sequencing (rLAS). [PDF]
Ura H, Hatanaka H, Togi S, Niida Y.
europepmc +1 more source
A Short-Read Amplicon Sequencing Protocol and Bioinformatic Pipeline for Ecological Surveillance of Dipteran Disease Vectors. [PDF]
Lima-Cordón R +18 more
europepmc +1 more source
Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research? [PDF]
Lyte JM +18 more
europepmc +1 more source
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Amplicon Sequencing Pipelines in Metagenomics
2023Taxonomic profiling among a large number of samples is a fundamental task during amplicon sequencing analysis. The heterogeneity and technical noises in the sample handling, library preparation, and sequencing present a major challenge to how the biological conclusions are drawn from the data analysis, and accordingly, many tools have been developed to
openaire +2 more sources
Practical innovations for high-throughput amplicon sequencing
Nature Methods, 2013We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide.
Derek S, Lundberg +4 more
openaire +2 more sources

