Results 51 to 60 of about 165,809 (296)

Single-amplicon samples sequencing statistics per amplicon.

, 2018
Single-amplicon samples sequencing statistics per amplicon.
Thomas K. F. Wong (361423), Allen G. Rodrigo (255318), Louis Ranjard (170210)   +2 more
core   +1 more source

Accurate multiplexing and filtering for high-throughput amplicon-sequencing [PDF]

Nucleic Acids Research, 2015
Tagging amplicons with tag sequences appended to PCR primers allow the multiplexing of numerous samples for high-throughput sequencing (HTS). This approach is routinely used in HTS-based diversity analyses, especially in microbial ecology and biomedical diagnostics.
Esling, Philippe, Lejzerowicz, Franck, Pawlowski, Jan   +2 more
openaire   +4 more sources

MinION sequence data: MinION sequencing of colorectal cancer tumor microbiomes – a comparison with amplicon-based and RNA-Sequencing

, 2019
MinION sequencing data that was unmapped by minimap2 for the 11 samples using in the "MinION sequencing of colorectal cancer tumor microbiomes – a comparison with amplicon-based and RNA-Sequencing" paper.
Taylor, William (5582345), Taylor, William, Purcell, Rachel, Purcell, Rachel (5791469)   +3 more
core   +1 more source

Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures

iScience, 2020
Summary: Synthetic bacterial communities are powerful tools for studying microbial ecology and evolution, as they enable rapid iteration between controlled laboratory experiments and theoretical modeling. However, their utility is hampered by the lack of
Nathan Cermak, Manoshi Sen Datta, Arolyn Conwill   +2 more
doaj   +1 more source

Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis

Frontiers in Microbiology, 2022
The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA ...
Hiroto Miura, Masayuki Takeda, Megumi Yamaguchi, Yoshihisa Ohtani, Go Endo, Yasuhisa Masuda, Kaede Ito, Yoshio Nagura, Kunihiro Iwashita, Tomohiro Mitani, Yutaka Suzuki, Yasuo Kobayashi, Satoshi Koike   +12 more
doaj   +1 more source

Nanopore amplicon sequencing v2

, 2022
This protocol describes the step-by-step procedures for performing nanopore amplicon sequencing using the latest Kit 14 chemistry. The method is versatile and can be applied to a wide range of sequence-based analyses, including human microbiome profiling and biodiversity assessment using environmental DNA (eDNA metabarcoding).
openaire   +1 more source

Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

BMC Microbiology, 2022
Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food.
Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo, Sandeep Tamber   +8 more
doaj   +1 more source

Amplicon Sequencing Files

, 2015
All 12 samples.
Xavier Pochon (160365), Dowle, Eddy J., Jonathan C. Banks (724674), Eddy J. Dowle (3256140), Karen Shearer (3256134), Shearer, Karen, Susanna A. Wood (453952), Wood, Susanna A., Pochon, Xavier, Banks, Jonathan C.   +9 more
core   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Amplicon sequencing of bacterial microbiota in abortion material from cattle [PDF]

, 2017
Abortions in cattle have a significant economic impact on animal husbandry and require prompt diagnosis for surveillance of epizootic infectious agents.
Sara Vidal, Kegler Schukovsky, Kristel, Vincent Perreten, Rodriguez, Sabrina, Vidal Lopez, Sara, Kristel Kegler, Sabrina Rodriguez-Campos, Posthaus, Horst, Horst Posthaus, Perreten, Vincent   +9 more
core   +1 more source