Results 51 to 60 of about 860,088 (184)

Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

BMC Microbiology, 2022
Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food.
Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo, Sandeep Tamber   +8 more
doaj   +1 more source

Amplicon Sequencing Files

, 2015
All 12 samples.
Xavier Pochon (160365), Dowle, Eddy J., Jonathan C. Banks (724674), Eddy J. Dowle (3256140), Karen Shearer (3256134), Shearer, Karen, Susanna A. Wood (453952), Wood, Susanna A., Pochon, Xavier, Banks, Jonathan C.   +9 more
core   +1 more source

Large-scale differences in microbial biodiversity discovery between 16S amplicon and shotgun sequencing

Scientific Reports, 2017
Modern metagenomic environmental DNA studies are almost completely reliant on next-generation sequencing, making evaluations of these methods critical. We compare two next-generation sequencing techniques – amplicon and shotgun – on water samples across ...
Michael Tessler, Johannes S. Neumann, Ebrahim Afshinnekoo, Michael Pineda, Rebecca Hersch, Luiz Felipe M. Velho, Bianca T. Segovia, Fabio A. Lansac-Toha, Michael Lemke, Rob DeSalle, Christopher E. Mason, Mercer R. Brugler   +11 more
doaj   +1 more source

Taxonomic assessment of rumen microbiota using total RNA and targeted amplicon sequencing approaches

, 2016
Taxonomic characterization of active gastrointestinal microbiota is essential to detect shifts in microbial communities and functions under various conditions.
Sun, Xu, Cox, Faith, Guan, Leluo, Li, Fuyong, Janssen, Peter H., Henderson, Gemma   +5 more
core   +1 more source

Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]

Nucleic Acids Research, 2015
With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics ...
Schirmer, Melanie, Ijaz, Umer Z., D'Amore, Rosalinda, Hall, Neil, Sloan, William T., Quince, Christopher   +5 more
openaire   +4 more sources

High-throughput amplicon sequencing of gut microbiome sea cucumber in Pahang, Malaysia [PDF]

, 2023
Sea cucumbers are soft-bodied marine organisms found in the benthic environment which are known as trepang, gamat or timun laut by locals. Sea cucumbers are commercially exploited for their body extracts due to their therapeutic properties and as ...
Shamrulazhar, Shamzir Kamal, Dennis Sandris, Nielsen, Shamrulazhar Shamzir, Kamal, Muhammad Fitri, Yusof, Choo, Mei-Yen, Nielsen, Dennis Sandris, Hajar Fauzan, Ahmad, Cho, Mei Yen   +7 more
core   +1 more source

Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer [PDF]

Gigascience, 2015
Abstract Background The MinION™ nanopore sequencer was recently released to a community of alpha-testers for evaluation using a variety of sequencing applications. Recent reports have tested the ability of the MinION™ to act as a whole genome sequencer and have demonstrated that nanopore sequencing ...
Kilianski, Andy, Haas, Jamie L, Corriveau, Elizabeth J, Liem, Alvin T, Willis, Kristen L, Kadavy, Dana R, Rosenzweig, C Nicole, Minot, Samuel S   +7 more
openaire   +2 more sources

Detection of low-density Plasmodium falciparum infections using amplicon deep sequencing

Malaria Journal, 2019
Background Deep sequencing of targeted genomic regions is becoming a common tool for understanding the dynamics and complexity of Plasmodium infections, but its lower limit of detection is currently unknown.
Angela M. Early, Rachel F. Daniels, Timothy M. Farrell, Jonna Grimsby, Sarah K. Volkman, Dyann F. Wirth, Bronwyn L. MacInnis, Daniel E. Neafsey   +7 more
doaj   +1 more source

Rapid, Inexpensive Measurement of Synthetic Bacterial Community Composition by Sanger Sequencing of Amplicon Mixtures

iScience, 2020
Summary: Synthetic bacterial communities are powerful tools for studying microbial ecology and evolution, as they enable rapid iteration between controlled laboratory experiments and theoretical modeling. However, their utility is hampered by the lack of
Nathan Cermak, Manoshi Sen Datta, Arolyn Conwill   +2 more
doaj   +1 more source

Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis

Frontiers in Microbiology, 2022
The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA ...
Hiroto Miura, Masayuki Takeda, Megumi Yamaguchi, Yoshihisa Ohtani, Go Endo, Yasuhisa Masuda, Kaede Ito, Yoshio Nagura, Kunihiro Iwashita, Tomohiro Mitani, Yutaka Suzuki, Yasuo Kobayashi, Satoshi Koike   +12 more
doaj   +1 more source