Results 41 to 50 of about 165,809 (296)

Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infections

BMC Genomics, 2017
Background Amplicon deep sequencing permits sensitive detection of minority clones and improves discriminatory power for genotyping multi-clone Plasmodium falciparum infections.
Anita Lerch, Cristian Koepfli, Natalie E. Hofmann, Camilla Messerli, Stephen Wilcox, Johanna H. Kattenberg, Inoni Betuela, Liam O’Connor, Ivo Mueller, Ingrid Felger   +9 more
doaj   +1 more source

Study of microbiocenosis of canine dental biofilms

Scientific Reports, 2021
Dental biofilm is a complex microbial community influenced by many exogenous and endogenous factors. Despite long-term studies, its bacterial composition is still not clearly understood.
Jana Kačírová, Aladár Maďari, Rastislav Mucha, Lívia K. Fecskeová, Izabela Mujakic, Michal Koblížek, Radomíra Nemcová, Marián Maďar   +7 more
doaj   +1 more source

Comparison between Southern blot and deep amplicon sequencing.

, 2023
A, DTUs identified in blood samples of 28 patients by Southern blot by using mHVR probes (left) and deep amplicon sequencing by using the 95% reference set.
Anahí G. Díaz (17382914), Tatiana Ponce (17382917), Patricio Diosque (216764), Christian Barnabé (283588), Fanny Rusman (6889418), Nicolás Tomasini (252330), Noelia Floridia-Yapur (8384577)   +6 more
core   +1 more source

Multiplex PCR Targeted Amplicon Sequencing (MTA-Seq): Simple, Flexible, and Versatile SNP Genotyping by Highly Multiplexed PCR Amplicon Sequencing

Frontiers in Plant Science, 2018
Next-generation sequencing (NGS) technologies have enabled genome re-sequencing for exploring genome-wide polymorphisms among individuals, as well as targeted re-sequencing for the rapid and simultaneous detection of polymorphisms in genes associated ...
Yoshihiko Onda, Yoshihiko Onda, Kotaro Takahagi, Kotaro Takahagi, Kotaro Takahagi, Minami Shimizu, Komaki Inoue, Keiichi Mochida, Keiichi Mochida, Keiichi Mochida   +9 more
doaj   +1 more source

Comprehensive evaluation of shotgun metagenomics, amplicon sequencing, and harmonization of these platforms for epidemiological studies [PDF]

, 2023
In a large cohort of 1,772 participants from the Hispanic Community Health Study/Study of Latinos with overlapping 16SV4 rRNA gene (bacterial amplicon), ITS1 (fungal amplicon), and shotgun sequencing data, we demonstrate that 16SV4 amplicon sequencing ...
Sollecito, Christopher C, Gellman, Marc D, Daviglus, Martha L, Burk, Robert D, Peters, Brandilyn A, Talavera, Gregory A, McDonald, Daniel, Shaffer, Justin P, Knight, Rob, Usyk, Mykhaylo, Thyagarajan, Bharat, Kaplan, Robert, Qi, Qibin, Karthikeyan, Smruthi, Vazquez-Baeza, Yoshiki   +14 more
core   +1 more source

Sanger sequencing of PCR amplicon of FPPS and PcWRKY44

, 2022
Sanger sequencing of PCR amplicon of FPPS and PcWRKY44 
Likai Chen (6101516)
core   +1 more source

Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]

Nucleic Acids Research, 2015
With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics ...
Schirmer, Melanie, Ijaz, Umer Z., D'Amore, Rosalinda, Hall, Neil, Sloan, William T., Quince, Christopher   +5 more
openaire   +4 more sources

Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer [PDF]

Gigascience, 2015
Abstract Background The MinION™ nanopore sequencer was recently released to a community of alpha-testers for evaluation using a variety of sequencing applications. Recent reports have tested the ability of the MinION™ to act as a whole genome sequencer and have demonstrated that nanopore sequencing ...
Kilianski, Andy, Haas, Jamie L, Corriveau, Elizabeth J, Liem, Alvin T, Willis, Kristen L, Kadavy, Dana R, Rosenzweig, C Nicole, Minot, Samuel S   +7 more
openaire   +2 more sources

PVAmpliconFinder: a workflow for the identification of human papillomaviruses from high-throughput amplicon sequencing

BMC Bioinformatics, 2020
Background The detection of known human papillomaviruses (PVs) from targeted wet-lab approaches has traditionally used PCR-based methods coupled with Sanger sequencing.
Alexis Robitaille, Rosario N. Brancaccio, Sankhadeep Dutta, Dana E. Rollison, Marcis Leja, Nicole Fischer, Adam Grundhoff, Tarik Gheit, Massimo Tommasino, Magali Olivier   +9 more
doaj   +1 more source

Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing

, 2012
Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa-a phenomenon termed ...
Polson, Shawn W., Shawn W. Polson (139348), Craig W. Herbold (139346), Charles K Lee, Ian R McDonald, S Craig Cary, K Eric Wommack, K. Eric Wommack (139352), Charles K. Lee (139341), Ian R. McDonald (139355), Herbold, Craig W., Lee, Charles Kai-Wu, Shawn W Polson, Shannon J. Williamson (38225), Williamson, Shannon J., Cary, S. Craig, S. Craig Cary (133811), Craig W Herbold, Shannon J Williamson, McDonald, Ian R., Wommack, K. Eric   +20 more
core   +1 more source