Results 41 to 50 of about 860,088 (184)

An Economical and Flexible Dual Barcoding, Two-Step PCR Approach for Highly Multiplexed Amplicon Sequencing

Frontiers in Microbiology, 2021
In microbiome research, phylogenetic and functional marker gene amplicon sequencing is the most commonly-used community profiling approach. Consequently, a plethora of protocols for the preparation and multiplexing of samples for amplicon sequencing have
Petra Pjevac, Petra Pjevac, Bela Hausmann, Bela Hausmann, Jasmin Schwarz, Jasmin Schwarz, Gudrun Kohl, Gudrun Kohl, Craig W. Herbold, Alexander Loy, Alexander Loy, David Berry, David Berry   +12 more
doaj   +1 more source

Determining the diet of larvae of western Rock Lobster (Panulirus cygnus) using high-throughput DNA sequencing techniques [PDF]

, 2012
The Western Australian rock lobster fishery has been both a highly productive and sustainable fishery. However, a recent dramatic and unexplained decline in post-larval recruitment threatens this sustainability.
Waite, A.M., Haruko Takeyama, Peter A Thompson, Lynnath E Beckley, Takeyama, H., Thompson, P.A., Tsai, P., Richard O'Rorke, Anya M Waite, Beckley, L.E., Shane Lavery, Seinen Chow, Andrew G Jeffs, Jeffs, A.G., O'Rorke, R., Lavery, S., Chow, S., Peter Tsai   +17 more
core   +1 more source

PVAmpliconFinder: a workflow for the identification of human papillomaviruses from high-throughput amplicon sequencing

BMC Bioinformatics, 2020
Background The detection of known human papillomaviruses (PVs) from targeted wet-lab approaches has traditionally used PCR-based methods coupled with Sanger sequencing.
Alexis Robitaille, Rosario N. Brancaccio, Sankhadeep Dutta, Dana E. Rollison, Marcis Leja, Nicole Fischer, Adam Grundhoff, Tarik Gheit, Massimo Tommasino, Magali Olivier   +9 more
doaj   +1 more source

Single-amplicon samples sequencing statistics per amplicon.

, 2018
Single-amplicon samples sequencing statistics per amplicon.
Thomas K. F. Wong (361423), Allen G. Rodrigo (255318), Louis Ranjard (170210)   +2 more
core   +1 more source

Comparison between Southern blot and deep amplicon sequencing.

, 2023
A, DTUs identified in blood samples of 28 patients by Southern blot by using mHVR probes (left) and deep amplicon sequencing by using the 95% reference set.
Anahí G. Díaz (17382914), Tatiana Ponce (17382917), Patricio Diosque (216764), Christian Barnabé (283588), Fanny Rusman (6889418), Nicolás Tomasini (252330), Noelia Floridia-Yapur (8384577)   +6 more
core   +1 more source

kmooog/UMI-amplicon: v1.0

, 2020
This version was used for the UMI project's 721 SSU rRNA amplicon data ...
kmooog
core   +2 more sources

Nanopore amplicon sequencing v2

, 2022
This protocol describes the step-by-step procedures for performing nanopore amplicon sequencing using the latest Kit 14 chemistry. The method is versatile and can be applied to a wide range of sequence-based analyses, including human microbiome profiling and biodiversity assessment using environmental DNA (eDNA metabarcoding).
openaire   +1 more source

Comprehensive evaluation of shotgun metagenomics, amplicon sequencing, and harmonization of these platforms for epidemiological studies [PDF]

, 2023
In a large cohort of 1,772 participants from the Hispanic Community Health Study/Study of Latinos with overlapping 16SV4 rRNA gene (bacterial amplicon), ITS1 (fungal amplicon), and shotgun sequencing data, we demonstrate that 16SV4 amplicon sequencing ...
Sollecito, Christopher C, Gellman, Marc D, Daviglus, Martha L, Burk, Robert D, Peters, Brandilyn A, Talavera, Gregory A, McDonald, Daniel, Shaffer, Justin P, Knight, Rob, Usyk, Mykhaylo, Thyagarajan, Bharat, Kaplan, Robert, Qi, Qibin, Karthikeyan, Smruthi, Vazquez-Baeza, Yoshiki   +14 more
core   +1 more source

MinION sequence data: MinION sequencing of colorectal cancer tumor microbiomes – a comparison with amplicon-based and RNA-Sequencing

, 2019
MinION sequencing data that was unmapped by minimap2 for the 11 samples using in the "MinION sequencing of colorectal cancer tumor microbiomes – a comparison with amplicon-based and RNA-Sequencing" paper.
Taylor, William (5582345), Taylor, William, Purcell, Rachel, Purcell, Rachel (5791469)   +3 more
core   +1 more source

Sanger sequencing of PCR amplicon of FPPS and PcWRKY44

, 2022
Sanger sequencing of PCR amplicon of FPPS and PcWRKY44 
Likai Chen (6101516)
core   +1 more source