Results 21 to 30 of about 165,809 (296)

A rapid, cost-effective tailed amplicon method for sequencing SARS-CoV-2

BMC Genomics, 2020
Background The global COVID-19 pandemic has led to an urgent need for scalable methods for clinical diagnostics and viral tracking. Next generation sequencing technologies have enabled large-scale genomic surveillance of SARS-CoV-2 as thousands of ...
Daryl M. Gohl, John Garbe, Patrick Grady, Jerry Daniel, Ray H. B. Watson, Benjamin Auch, Andrew Nelson, Sophia Yohe, Kenneth B. Beckman   +8 more
doaj   +1 more source

Predicting environmental stressor levels with machine learning: a comparison between amplicon sequencing, metagenomics, and total RNA sequencing based on taxonomically assigned data

Frontiers in Microbiology, 2023
IntroductionMicrobes are increasingly (re)considered for environmental assessments because they are powerful indicators for the health of ecosystems.
Christopher A. Hempel, Christopher A. Hempel, Dominik Buchner, Leoni Mack, Marie V. Brasseur, Dan Tulpan, Dan Tulpan, Florian Leese, Florian Leese, Dirk Steinke, Dirk Steinke   +10 more
doaj   +1 more source

Grinder: a versatile amplicon and shotgun sequence simulator [PDF]

Nucleic Acids Research, 2012
We introduce Grinder (http://sourceforge.net/projects/biogrinder/), an open-source bioinformatic tool to simulate amplicon and shotgun (genomic, metagenomic, transcriptomic and metatranscriptomic) datasets from reference sequences. This is the first tool to simulate amplicon datasets (e.g. 16S rRNA) widely used by microbial ecologists.
Angly, Florent E., Willner, Dana, Rohwer, Forest, Hugenholtz, Philip, Tyson, Gene W.   +4 more
openaire   +5 more sources

Unusual sequence element found at the end of an amplicon. [PDF]

Molecular and Cellular Biology, 1987
In a polyomavirus-transformed rat cell line, designated LPT, the polyomavirus DNA is integrated into a single chromosomal site. Treatment of LPT cells with carcinogens induces amplification of the integrated virus DNA and flanking cellular sequences.
N, Baran, A, Lapidot, H, Manor
openaire   +2 more sources

Nanopore Is Preferable over Illumina for 16S Amplicon Sequencing of the Gut Microbiota When Species-Level Taxonomic Classification, Accurate Estimation of Richness, or Focus on Rare Taxa Is Required

Microorganisms, 2023
Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon sequencing as it can provide full-length 16S reads and has a low up-front cost that allows research groups to set up their own sequencing workflows.
Márton Szoboszlay, Laetitia Schramm, David Pinzauti, Jeanesse Scerri, Anna Sandionigi, Manuele Biazzo   +5 more
doaj   +1 more source

High-throughput amplicon sequencing of gut microbiome sea cucumber in Pahang, Malaysia [PDF]

, 2023
Sea cucumbers are soft-bodied marine organisms found in the benthic environment which are known as trepang, gamat or timun laut by locals. Sea cucumbers are commercially exploited for their body extracts due to their therapeutic properties and as ...
Shamrulazhar, Shamzir Kamal, Dennis Sandris, Nielsen, Shamrulazhar Shamzir, Kamal, Muhammad Fitri, Yusof, Choo, Mei-Yen, Nielsen, Dennis Sandris, Hajar Fauzan, Ahmad, Cho, Mei Yen   +7 more
core   +1 more source

Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples

Genome Medicine, 2020
Background COVID-19 (coronavirus disease 2019) has caused a major epidemic worldwide; however, much is yet to be known about the epidemiology and evolution of the virus partly due to the scarcity of full-length SARS-CoV-2 (severe acute respiratory ...
Minfeng Xiao, Xiaoqing Liu, Jingkai Ji, Min Li, Jiandong Li, Lin Yang, Wanying Sun, Peidi Ren, Guifang Yang, Jincun Zhao, Tianzhu Liang, Huahui Ren, Tian Chen, Huanzi Zhong, Wenchen Song, Yanqun Wang, Ziqing Deng, Yanping Zhao, Zhihua Ou, Daxi Wang, Jielun Cai, Xinyi Cheng, Taiqing Feng, Honglong Wu, Yanping Gong, Huanming Yang, Jian Wang, Xun Xu, Shida Zhu, Fang Chen, Yanyan Zhang, Weijun Chen, Yimin Li, Junhua Li   +33 more
doaj   +1 more source

Nanoliter-scale next-generation sequencing library-mediated high-throughput 16S rRNA microbial community profiling

BioTechniques, 2020
An ultra-high-throughput workflow for next-generation sequencing library construction at nanoliter scale for amplicon sequencing, termed Smartchip Nanowell Platform for Target Enrichment, was established using a nanodispenser system and a nanoliter-scale
Hui Zhang, Xiangdan Yu, Zhe Zhang, Zhenhua Liu, Cong Tang, Kun Zhao, Shiyan Liu, Yong Sun, Xiang Li, Zijing Xu, Hong Zhang, Keqing Shi, Yan Fu   +12 more
doaj   +1 more source

noelzach/Oomycete-Amplicon-Seq-Soybean-Rhizosphere: getting zenodo

, 2023
Manuscript about amplicon sequencing of oomycetes from soybean ...
Zachary Noel
core   +1 more source

Comprehensive Genome Sequence Analysis of a Breast Cancer Amplicon [PDF]

Genome Research, 2001
Gene amplification occurs in most solid tumors and is associated with poor prognosis. Amplification of 20q13.2 is common to several tumor types including breast cancer. The 1 Mb of sequence spanning the 20q13.2 breast cancer amplicon is one of the most exhaustively studied segments of the human genome.
Collins, Colin, Volik, Stanislav, Kowbel, David, Ginzinger, David, Ylstra, Bauke, Cloutier, Thomas, Hawkins, Trevor, Predki, Paul, Martin, Christopher, Wernick, Meredith, Kuo, Wen Lin, Alberts, Arthur, Gray, Joe W.   +12 more
openaire   +2 more sources