Results 1 to 10 of about 165,809 (296)

Microbial diagnosis of endophthalmitis using nanopore amplicon sequencing

International Journal of Medical Microbiology, 2021
Objectives: We investigated whether nanopore amplicon sequencing of aqueous humor was capable of rapid pathogen identification in infectious endophthalmitis.
Kang Il Jun, Baek-Lok Oh, Narae Kim
exaly   +5 more sources

A Sequel to Sanger: amplicon sequencing that scales [PDF]

BMC Genomics, 2018
Background Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing.
Paul D. N. Hebert, Thomas W. A. Braukmann, Sean W. J. Prosser, Sujeevan Ratnasingham, Jeremy R. deWaard, Natalia V. Ivanova, Daniel H. Janzen, Winnie Hallwachs, Suresh Naik, Jayme E. Sones, Evgeny V. Zakharov   +10 more
doaj   +4 more sources

Pathways for Understanding Blue Carbon Microbiomes with Amplicon Sequencing [PDF]

Microorganisms, 2022
The capacity of Blue Carbon Ecosystems to act as carbon sinks is strongly influenced by the metabolism of soil-associated microbes, which ultimately determine how much carbon is accumulated or returned to the atmosphere. The rapid evolution of sequencing
Bowen, JL, Macreadie, PI, Rod M. Connolly, Duarte, CM, Stacey M. Trevathan-Tackett, Carlos M. Duarte, Trevathan-Tackett, Stacey M., Trevathan-Tackett, Stacey M, Valentina Hurtado-McCormick, Macreadie, Peter I., Macreadie, Peter, Connolly, RM, Bowen, Jennifer L, Bowen, Jennifer L., Macreadie, Peter I, Jennifer L. Bowen, Hurtado-McCormick, Valentina, Connolly, Rod M., Peter I. Macreadie, Trevathan-Tackett, SM, Duarte, Carlos M, Connolly, Rod M, Hurtado-McCormick, V, Duarte, Carlos M.   +23 more
core   +5 more sources

MinION Sequencing of colorectal cancer tumour microbiomes-A comparison with amplicon-based and RNA-Sequencing.

PLoS ONE, 2020
BACKGROUND:Recent evidence suggests a role for the gut microbiome in the development and progression of many diseases and many studies have been carried out to analyse the microbiome using a variety of methods. In this study, we compare MinION sequencing
William S Taylor, John Pearson, Allison Miller, Sebastian Schmeier, Frank A Frizelle, Rachel V Purcell   +5 more
doaj   +2 more sources

Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform

MSystems, 2019
Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in ...
Johanna B Holm, Michael S Humphrys, Elias Mccomb   +2 more
exaly   +3 more sources

Development of a qPCR assay for Fasciola spp. identification and a deep amplicon sequencing method for differentiation of fluke species in UK livestock. [PDF]

PLoS Neglected Tropical Diseases
BackgroundTrematode parasites, or flukes, are a significant economic threat to ruminant production worldwide. Traditional diagnostic methods rely on egg sedimentation from faeces, a time-consuming methodology lacking sensitivity and specificity.
Muhammad Abbas, Kezia Kozel, Olukayode Daramola, Nick Selemetas, Qasim Ali, Shoaib Ashraf, Ibrahim Isah, Inaki Deza-Cruz, Sai Fingerhood, Mark W Robinson, Eric R Morgan, Umer Chaudhry, Martha Betson   +12 more
doaj   +2 more sources

Amplicon-based metagenomic analysis of mixed fungal samples using proton release amplicon sequencing. [PDF]

PLoS ONE, 2014
Next generation sequencing technology has revolutionised microbiology by allowing concurrent analysis of whole microbial communities. Here we developed and verified similar methods for the analysis of fungal communities using a proton release sequencing ...
Daniel P Tonge, Catherine H Pashley, Timothy W Gant   +2 more
doaj   +5 more sources

16S rRNA Amplicon Sequencing

, 2023
The 16S rRNA amplicon sequencing technique is a microbiome analysis where different samples are analyzed at the same time using multiplexing. The results can be used to evaluate microbial diversity at genus, family, order, class, and phylum levels.
Andersson, Jasmine, Vogt, Josef Korbinian, Jørgensen, Steffen Lynge, Christensen, Henrik   +3 more
core   +2 more sources

An Economical and Flexible Dual Barcoding, Two-Step PCR Approach for Highly Multiplexed Amplicon Sequencing

Frontiers in Microbiology, 2021
In microbiome research, phylogenetic and functional marker gene amplicon sequencing is the most commonly-used community profiling approach. Consequently, a plethora of protocols for the preparation and multiplexing of samples for amplicon sequencing have
Petra Pjevac, Bela Hausmann, Craig W Herbold   +2 more
exaly   +3 more sources

Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix) [PDF]

PeerJ, 2019
Next-generation sequencing (NGS) of amplicons is used in a wide variety of contexts. In many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library preparation strategies relying upon the fusion of locus-specific primers to ...
Travis C. Glenn, Todd W. Pierson, Natalia J. Bayona-Vásquez, Troy J. Kieran, Sandra L. Hoffberg, Jesse C. Thomas IV, Daniel E. Lefever, John W. Finger, Bei Gao, Xiaoming Bian, Swarnali Louha, Ramya T. Kolli, Kerin E. Bentley, Julie Rushmore, Kelvin Wong, Timothy I. Shaw, Michael J. Rothrock Jr, Anna M. McKee, Tai L. Guo, Rodney Mauricio, Marirosa Molina, Brian S. Cummings, Lawrence H. Lash, Kun Lu, Gregory S. Gilbert, Stephen P. Hubbell, Brant C. Faircloth   +26 more
doaj   +5 more sources