Results 11 to 20 of about 165,809 (296)

EasyAmplicon 2: Expanding PacBio and Nanopore Long Amplicon Sequencing Analysis Pipeline for Microbiome [PDF]

Advanced Science
In the past decade, third‐generation sequencing technologies (such as PacBio (Pacific Biosciences) and Nanopore) have become gradually matured and are widely used for microbial taxonomy and quantification.
Hao Luo, Defeng Bai, Zhihao Zhu, Salsabeel Yousuf, Haifei Yang, Jiani Xun, Meiyin Zeng, Yao Wang, Yunyun Gao, Kai Peng, Shanshan Xu, Yuanping Zhou, Tianyuan Zhang, Chuang Ma, Huiyu Hou, Xiulin Wan, Yang Zhou, Baolei Jia, Shi Huang, Renyou Gan, Tao Wen, Tong Chen, Xia Chen, Xiaofang Li, Yong‐Xin Liu   +24 more
doaj   +2 more sources

Triplicate PCR Reactions for 16S rRNA Gene Amplicon Sequencing are Unnecessary

BioTechniques, 2019
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation.
Clarisse Marotz, Anukriti Sharma, Greg Humphrey   +2 more
exaly   +3 more sources

MHASS: Microbiome HiFi Amplicon Sequencing Simulator. [PDF]

Bioinformatics
Abstract Summary Microbiome HiFi Amplicon Sequence Simulator (MHASS) creates realistic synthetic PacBio HiFi amplicon sequencing datasets for microbiome studies, by integrating genome-aware abundance modeling, realistic dual-barcoding strategies, and empirically derived pass-number ...
Howard-Stone R, Măndoiu II.
europepmc   +4 more sources

Rapid intraoperative amplicon sequencing of CNS tumor markers

Computational and Structural Biotechnology Journal
Currently, central nervous system tumors are diagnosed with an integrated diagnostic approach that combines histopathological examination with molecular genetic profiling, which requires days to weeks to achieve a precise and informative classification ...
Maximilian Evers, Björn Brändl, Christian Rohrandt, Carolin Kubelt-Kwamin, Franz-Josef Müller, Dominik Danso, André Maicher, Gaojianyong Wang, Sönke Friedrichsen, Stephan Kolkenbrock   +9 more
doaj   +4 more sources

Dissecting Disease-Suppressive Rhizosphere Microbiomes by Functional Amplicon Sequencing and 10x Metagenomics [PDF]

, 2021
Disease-suppressive soils protect plants against soilborne fungal pathogens that would otherwise cause root infections. Soil suppressiveness is, in most cases, mediated by the antagonistic activity of the microbial community associated with the plant ...
Tracanna, V., Warris, S., Ossowicki, A., Lund, G., Medema, M. H., Terlouw, B. R., Garbeva, P., Oversuin, S., Robinson, S. L., Raaijmakers, J. M., Van Wezel, G. P., Schijlen, E. G. W. M., Petrus, M. L. C.   +12 more
core   +1 more source

Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections

Frontiers in Cellular and Infection Microbiology, 2022
Cancer patients are at high risk of infections and infection-related mortality; thereby, prompt diagnosis and precise anti-infectives treatment are critical.
Qingmei Deng, Qingmei Deng, Qingmei Deng, Yongqing Cao, Xiaofeng Wan, Xiaofeng Wan, Bin Wang, Aimin Sun, Aimin Sun, Huanzhong Wang, Huanzhong Wang, Yunfei Wang, Hongzhi Wang, Hongzhi Wang, Hongzhi Wang, Hongcang Gu, Hongcang Gu, Hongcang Gu   +17 more
doaj   +1 more source

Evaluating the Reproducibility of Amplicon Sequencing Data Derived from Deep-Sea Cold Seep Sediment-Associated Microbiota

Microbiology Spectrum, 2023
Benefiting from the rapid developments and wide applications of high-throughput sequencing, great advancements have been made in investigating microbiota, which are highly diverse and play key roles in both element cycling and the energy flow of ...
Jie Kong, Jingchun Feng, Liwei Sun, Si Zhang   +3 more
doaj   +1 more source

Establishment and assessment of an amplicon sequencing method targeting the 16S-ITS-23S rRNA operon for analysis of the equine gut microbiome

Scientific Reports, 2021
Microbial communities are commonly studied by using amplicon sequencing of part of the 16S rRNA gene. Sequencing of the full-length 16S rRNA gene can provide higher taxonomic resolution and accuracy.
Yuta Kinoshita, Hidekazu Niwa, Eri Uchida-Fujii, Toshio Nukada   +3 more
doaj   +1 more source

SESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing [PDF]

Bioinformatics, 2010
Abstract Summary: Characterizing genetic diversity through genotyping short amplicons is central to evolutionary biology. Next-generation sequencing (NGS) technologies changed the scale at which these type of data are acquired. SESAME is a web application package that assists genotyping of multiplexed individuals for several markers ...
Meglécz, Emese, Piry, Sylvain, Desmarais, Erick, Galan, Maxime, Gilles, André, Guivier, Emmanuel, Pech, Nicolas, Martin, Jean-François   +7 more
openaire   +5 more sources

Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing

Veterinary Sciences, 2023
This study aimed to describe the bacterial composition of the surgical site during elective caesarean sections (CSs) using the 16Sr DNA amplicon sequencing performed in parallel to bacterial culture.
Salem Djebala, Elise Coria, Florian Munaut, Linde Gille, Justine Eppe, Nassim Moula, Bernard Taminiau, Georges Daube, Philippe Bossaert   +8 more
doaj   +1 more source