Results 11 to 20 of about 860,088 (184)

MinION Sequencing of colorectal cancer tumour microbiomes-A comparison with amplicon-based and RNA-Sequencing.

PLoS ONE, 2020
BACKGROUND:Recent evidence suggests a role for the gut microbiome in the development and progression of many diseases and many studies have been carried out to analyse the microbiome using a variety of methods. In this study, we compare MinION sequencing
William S Taylor, John Pearson, Allison Miller, Sebastian Schmeier, Frank A Frizelle, Rachel V Purcell   +5 more
doaj   +2 more sources

Pathways for Understanding Blue Carbon Microbiomes with Amplicon Sequencing [PDF]

Microorganisms, 2022
The capacity of Blue Carbon Ecosystems to act as carbon sinks is strongly influenced by the metabolism of soil-associated microbes, which ultimately determine how much carbon is accumulated or returned to the atmosphere. The rapid evolution of sequencing
Bowen, JL, Macreadie, PI, Rod M. Connolly, Duarte, CM, Stacey M. Trevathan-Tackett, Carlos M. Duarte, Trevathan-Tackett, Stacey M., Trevathan-Tackett, Stacey M, Valentina Hurtado-McCormick, Macreadie, Peter I., Macreadie, Peter, Connolly, RM, Bowen, Jennifer L, Bowen, Jennifer L., Macreadie, Peter I, Jennifer L. Bowen, Hurtado-McCormick, Valentina, Connolly, Rod M., Peter I. Macreadie, Trevathan-Tackett, SM, Duarte, Carlos M, Connolly, Rod M, Hurtado-McCormick, V, Duarte, Carlos M.   +23 more
core   +5 more sources

A Sequel to Sanger: amplicon sequencing that scales [PDF]

BMC Genomics, 2018
Background Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing.
Paul D. N. Hebert, Thomas W. A. Braukmann, Sean W. J. Prosser, Sujeevan Ratnasingham, Jeremy R. deWaard, Natalia V. Ivanova, Daniel H. Janzen, Winnie Hallwachs, Suresh Naik, Jayme E. Sones, Evgeny V. Zakharov   +10 more
doaj   +3 more sources

Amplicon-based metagenomic analysis of mixed fungal samples using proton release amplicon sequencing. [PDF]

PLoS ONE, 2014
Next generation sequencing technology has revolutionised microbiology by allowing concurrent analysis of whole microbial communities. Here we developed and verified similar methods for the analysis of fungal communities using a proton release sequencing ...
Daniel P Tonge, Catherine H Pashley, Timothy W Gant   +2 more
doaj   +5 more sources

16S rRNA Amplicon Sequencing

, 2023
The 16S rRNA amplicon sequencing technique is a microbiome analysis where different samples are analyzed at the same time using multiplexing. The results can be used to evaluate microbial diversity at genus, family, order, class, and phylum levels.
Andersson, Jasmine, Vogt, Josef Korbinian, Jørgensen, Steffen Lynge, Christensen, Henrik   +3 more
core   +2 more sources

Dissecting Disease-Suppressive Rhizosphere Microbiomes by Functional Amplicon Sequencing and 10x Metagenomics [PDF]

, 2021
Disease-suppressive soils protect plants against soilborne fungal pathogens that would otherwise cause root infections. Soil suppressiveness is, in most cases, mediated by the antagonistic activity of the microbial community associated with the plant ...
Tracanna, V., Warris, S., Ossowicki, A., Lund, G., Medema, M. H., Terlouw, B. R., Garbeva, P., Oversuin, S., Robinson, S. L., Raaijmakers, J. M., Van Wezel, G. P., Schijlen, E. G. W. M., Petrus, M. L. C.   +12 more
core   +1 more source

Rapid intraoperative amplicon sequencing of CNS tumor markers

Computational and Structural Biotechnology Journal
Currently, central nervous system tumors are diagnosed with an integrated diagnostic approach that combines histopathological examination with molecular genetic profiling, which requires days to weeks to achieve a precise and informative classification ...
Maximilian Evers, Björn Brändl, Christian Rohrandt, Carolin Kubelt-Kwamin, Franz-Josef Müller, Dominik Danso, André Maicher, Gaojianyong Wang, Sönke Friedrichsen, Stephan Kolkenbrock   +9 more
doaj   +4 more sources

MHASS: Microbiome HiFi Amplicon Sequencing Simulator. [PDF]

Bioinformatics
Abstract Summary Microbiome HiFi Amplicon Sequence Simulator (MHASS) creates realistic synthetic PacBio HiFi amplicon sequencing datasets for microbiome studies, by integrating genome-aware abundance modeling, realistic dual-barcoding strategies, and empirically derived pass-number ...
Howard-Stone R, Măndoiu II.
europepmc   +5 more sources

Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis. [PDF]

Microbiol Spectr
Abstract Most microbiome studies to date rely on sequencing short amplicons of the 16S rRNA gene on Illumina’s platforms. Because of the short read length, sequences often can be identified reliably only to the family or genus levels. Long read sequencing with whole-length 16S rRNA sequencing can improve taxonomic resolution, but often ...
O'Sullivan B, Herbst KW, Hogan AH, Maltz-Matyschsyk M, Radolf JD, Lawrence D, Lynes MA, Salazar JC, Graf J, Connecticut Children’s COVID Collaborative.   +9 more
europepmc   +3 more sources

SESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing [PDF]

Bioinformatics, 2010
Abstract Summary: Characterizing genetic diversity through genotyping short amplicons is central to evolutionary biology. Next-generation sequencing (NGS) technologies changed the scale at which these type of data are acquired. SESAME is a web application package that assists genotyping of multiplexed individuals for several markers ...
Meglécz, Emese, Piry, Sylvain, Desmarais, Erick, Galan, Maxime, Gilles, André, Guivier, Emmanuel, Pech, Nicolas, Martin, Jean-François   +7 more
openaire   +6 more sources