Results 31 to 40 of about 165,809 (296)

High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in

BMC Genomics, 2023
Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition.
Tejali Naik, Mohak Sharda, Lakshminarayanan C P, Kumar Virbhadra, Awadhesh Pandit   +4 more
doaj   +1 more source

Removing noise from pyrosequenced amplicons [PDF]

, 2011
<b>Background</b><p></p> In many environmental genomics applications a homologous region of DNA from a diverse sample is first amplified by PCR and then sequenced.
Quince, Christopher, Marie Ollier, Decousus, Stephanie, Davenport, Russell J., Christopher Quince, Davenport Russell J, Quince Christopher, Ollier, Marie, Sandrine Viala, Saghir, Nagi,, Uhrhammer, Nancy, Saghir, Nagi, S El, Turnbaugh, Peter, Davenport, Russell J, Turnbaugh, Peter J., Lanzen Anders, Ponelle, Flora, Nancy Uhrhammer, Russell J Davenport, Lanzen, Anders, Uhrhammer, Nancy A., Axel Poulet, Privat, Maud, Peter J Turnbaugh, Maud Privat, Axel Perin, Lafarge, Laurence, Bidet, Yannick, Laurence Lafarge, Stephanie Decousus, Poulet, Axel, Viala, Sandrine, Yannick Bidet, Nagi S. El Saghir, Yves-Jean Bignon, Bignon, Yves-Jean, Turnbaugh Peter J, El-Saghir, Nagi S., Flora Ponelle, Anders Lanzen, Perin, Axel, Bignon, Yves Jean   +41 more
core   +1 more source

AM fungal amplicon sequencing data.

, 2023
AM fungal amplicon sequencing data.
Wei Fu (10707882)
core   +1 more source

Strategy for Modular Tagged High-Throughput Amplicon Sequencing [PDF]

Applied and Environmental Microbiology, 2011
ABSTRACT The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.
de Carcer, Daniel Aguirre, Denman, Stuart E., McSweeney, Chris, Morrison, Mark   +3 more
openaire   +6 more sources

Development of an amplicon-based sequencing approach in response to the global emergence of mpox.

PLoS Biology, 2023
The 2022 multicountry mpox outbreak concurrent with the ongoing Coronavirus Disease 2019 (COVID-19) pandemic further highlighted the need for genomic surveillance and rapid pathogen whole-genome sequencing.
Nicholas F G Chen, Chrispin Chaguza, Luc Gagne, Matthew Doucette, Sandra Smole, Erika Buzby, Joshua Hall, Stephanie Ash, Rachel Harrington, Seana Cofsky, Selina Clancy, Curtis J Kapsak, Joel Sevinsky, Kevin Libuit, Daniel J Park, Peera Hemarajata, Jacob M Garrigues, Nicole M Green, Sean Sierra-Patev, Kristin Carpenter-Azevedo, Richard C Huard, Claire Pearson, Kutluhan Incekara, Christina Nishimura, Jian Ping Huang, Emily Gagnon, Ethan Reever, Jafar Razeq, Anthony Muyombwe, Vítor Borges, Rita Ferreira, Daniel Sobral, Silvia Duarte, Daniela Santos, Luís Vieira, João Paulo Gomes, Carly Aquino, Isabella M Savino, Karinda Felton, Moneeb Bajwa, Nyjil Hayward, Holly Miller, Allison Naumann, Ria Allman, Neel Greer, Amary Fall, Heba H Mostafa, Martin P McHugh, Daniel M Maloney, Rebecca Dewar, Juliet Kenicer, Abby Parker, Katharine Mathers, Jonathan Wild, Seb Cotton, Kate E Templeton, George Churchwell, Philip A Lee, Maria Pedrosa, Brenna McGruder, Sarah Schmedes, Matthew R Plumb, Xiong Wang, Regina Bones Barcellos, Fernanda M S Godinho, Richard Steiner Salvato, Aimee Ceniseros, Mallery I Breban, Nathan D Grubaugh, Glen R Gallagher, Chantal B F Vogels   +70 more
doaj   +1 more source

Sequencing statistics for full-length rRNA amplicon sequencing.

, 2021
Sequencing statistics for full-length rRNA amplicon sequencing.
Belgin Dogan (148469), Shiying Zhang (2813257), Rachel M. Bleich (822389), Jeremy Wang (383590), Sandra Zarmer (3804274), Kenneth W. Simpson (7388261), Janelle C. Arthur (10911444)   +6 more
core   +1 more source

Core of the saliva microbiome: an analysis of the MG-RAST data

BMC Oral Health, 2021
Background Oral microbiota is considered as the second most complex in the human body and its dysbiosis can be responsible for oral diseases. Interactions between the microorganism communities and the host allow establishing the microbiological proles ...
Simone G. Oliveira, Rafaela R. Nishiyama, Claudio A. C. Trigo, Ana Luiza Mattos-Guaraldi, Alberto M. R. Dávila, Rodrigo Jardim, Flavio H. B. Aguiar   +6 more
doaj   +1 more source

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Lutgarde Raskin (143205), Ameet J. Pinto (143202), Lutgarde Raskin, Pinto Ameet J., Raskin, L., Ameet J Pinto, Raskin Lutgarde, Pinto, A.   +7 more
core   +1 more source

An amplicon-based sequencing approach for the study of aeromycology

Journal of Xenobiotics, 2018
Not available.
Hamza Mbareche, Marc Veillette, Guillaume J. Bilodeau, Caroline Duchaine   +3 more
openaire   +4 more sources

Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform

mSystems, 2019
Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in ...
Johanna B. Holm, Michael S. Humphrys, Courtney K. Robinson, Matthew L. Settles, Sandra Ott, Li Fu, Hongqiu Yang, Pawel Gajer, Xin He, Elias McComb, Patti E. Gravitt, Khalil G. Ghanem, Rebecca M. Brotman, Jacques Ravel   +13 more
doaj   +1 more source