Interaction interface in the C-terminal parts of centriole proteins Sas6 and Ana2 [PDF]
Open Biology, 2020 The centriole is a ninefold symmetrical structure found at the core of centrosomes and, as a basal body, at the base of cilia, whose conserved duplication is regulated by Plk4 kinase.
Michał Dadlez +2 more
exaly +3 more sources
Drosophila Alms1 proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification loop [PDF]
EMBO JournalCentrioles play a central role in cell division by recruiting pericentriolar material (PCM) to form the centrosome. Alterations in centriole number or function lead to various diseases including cancer or microcephaly.
veronique Morel +2 more
exaly +3 more sources
GST-IVTT pull-down: a fast and versatile in vitro method for validating and mapping protein-protein interactions. [PDF]
FEBS Open Bio, 2022 The GST‐IVTT pull‐down assay is suitable for the rapid validation and mapping of functional protein interactions in vitro. The 35S‐labelled prey protein or its pieces (putative interactors) are produced by a single‐step IVTT (in vitro transcription/translation) reaction from various templates.
Réthi-Nagy Z, Ábrahám E, Lipinszki Z.
europepmc +2 more sources
Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation [PDF]
Open Biology, 2017 The conserved process of centriole duplication requires Plk4 kinase to recruit and promote interactions between Sas6 and Sas5/Ana2/STIL (respective nomenclature of worms/flies/humans).
Michał Dadlez +2 more
exaly +2 more sources
PLK4: Master Regulator of Centriole Duplication and Its Therapeutic Potential. [PDF]
Cytoskeleton (Hoboken)ABSTRACT Centrosomes catalyze the assembly of a microtubule‐based bipolar spindle, essential for the precise chromosome segregation during cell division. At the center of this process lies Polo‐Like Kinase 4 (PLK4), the master regulator that controls the duplication of the centriolar core to ensure the correct balance of two centrosomes per dividing ...
Hamzah M, Meitinger F, Ohta M.
europepmc +2 more sources
Post-translational modification sites are present in hydrophilic cavities of alpha-synuclein, tau, FUS, and TDP-43 fibrils: A molecular dynamics study. [PDF]
ProteinsAbstract Hydration plays a crucial role in the refolding of intrinsically disordered proteins into amyloid fibrils; however, the specific interactions between water and protein that may contribute to this process are still unknown. In our previous studies of alpha‐synuclein (aSyn), we have shown that waters confined in fibril cavities are stabilizing ...
Kochen NN +5 more
europepmc +2 more sources
Poc1B and Sas-6 Function Together during the Atypical Centriole Formation in Drosophila melanogaster [PDF]
Cells, 2019 Insects and mammals have atypical centrioles in their sperm. However, it is unclear how these atypical centrioles form. Drosophila melanogaster sperm has one typical centriole called the giant centriole (GC) and one atypical centriole called the proximal
Kyoung H. Jo +5 more
doaj +2 more sources
Residual flexibility in the topologically constrained multivalent complex between the GKAP scaffold and LC8 hub proteins. [PDF]
FEBS JThe scaffold protein GKAP and the hub protein LC8 form a well‐defined hexameric complex, with the stoichiometry of 2 GKAP : 2 LC8 dimers. NMR and molecular dynamics calculations confirm that the LC8‐binding segment of GKAP is intrinsically disordered even in the complex form.
Nagy-Kanta E +10 more
europepmc +2 more sources
Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL [PDF]
Journal of Cell Biology, 2022 Eline van Houtum +2 more
exaly +2 more sources
Author Correction: Drosophila Alms1 proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification loop [PDF]
The EMBO JournalMarine Brunet +8 more
doaj +2 more sources