eLife, 2015 Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved,
Matthew A Cottee +5 more
doaj +5 more sources
Drosophila Ana2 is a conserved centriole duplication factor [PDF]
Journal of Cell Biology, 2010 Drosophila protein Ana2 supports de novo formation of centrioles and may be a functional homologue of the C. elegans centriole duplication protein SAS-5.
Jeroen Dobbelaere +2 more
exaly +4 more sources
Plk4 Phosphorylates Ana2 to Trigger Sas6 Recruitment and Procentriole Formation [PDF]
Current Biology, 2014 Summary Centrioles are 9-fold symmetrical structures at the core of centrosomes and base of cilia whose dysfunction has been linked to a wide range of inherited diseases and cancer [1].
Nikola S Dzhindzhev +2 more
exaly +5 more sources
The Mechanism of Dynein Light Chain LC8-mediated Oligomerization of the Ana2 Centriole Duplication Factor [PDF]
Journal of Biological Chemistry, 2014 Background: Ana2 is a conserved centriole duplication factor involved in nascent centriole biogenesis. Results: Two sites in the central domain of Ana2 (Ana2M) bind LC8 and form an Ana2M4-LC88 complex.
Kevin C Slep
exaly +4 more sources
Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly. [PDF]
J Cell Sci, 2020 Centriole assembly requires a small number of conserved proteins. The precise pathway of centriole assembly has been difficult to study, as the lack of any one of the core assembly proteins [Plk4, Ana2 (the homologue of mammalian STIL), Sas-6, Sas-4 ...
Gartenmann L +6 more
europepmc +5 more sources
DSas-6 and Ana2 Coassemble into Tubules to Promote Centriole Duplication and Engagement
Developmental Cell, 2010 Summary Centrioles form cilia and centrosomes, organelles whose dysfunction is increasingly linked to human disease. Centriole duplication relies on a few conserved proteins (ZYG-1/Sak/Plk4, SAS-6, SAS-5/Ana2, and SAS-4), and is often initiated by the ...
Helio Roque, Jordan W Raff
exaly +5 more sources
Centriole Duplication: When PLK4 Meets Ana2/STIL [PDF]
Current Biology, 2014 Polo-like kinase 4 is known to drive centriole duplication, but the relevant substrate remains elusive. A new study shows that PLK4 phosphorylates a key centriolar component, Ana2/STIL, to initiate centriole assembly.
Chii Shyang Fong, Meng-Fu Bryan Tsou
exaly +4 more sources
An Ana2/Ctp/Mud Complex Regulates Spindle Orientation in Drosophila Neuroblasts [PDF]
Developmental Cell, 2011 Drosophila neural stem cells, larval brain neuroblasts (NBs), align their mitotic spindles along the apical/basal axis during asymmetric cell division (ACD) to maintain the balance of self-renewal and differentiation. Here, we identified a protein complex composed of the tumor suppressor anastral spindle 2 (Ana2), a dynein light-chain protein Cut up ...
Jens Januschke +2 more
exaly +5 more sources
SAPs as a new model to probe the pathway of centriole and centrosome assembly. [PDF]
Biochem Soc Trans, 2021 Centrioles are important cellular organelles involved in the formation of both cilia and centrosomes. It is therefore not surprising that their dysfunction may lead to a variety of human pathologies.
Wainman A.
europepmc +2 more sources
Generating CRISPR-edited clonal lines of cultured <i>Drosophila</i> S2 cells. [PDF]
Biol Methods ProtocCRISPR/Cas9 genome editing is a pervasive research tool due to its relative ease of use. However, some systems are not amenable to generating edited clones due to genomic complexity and/or difficulty in establishing clonal lines.
Ryniawec JM, Amoiroglou A, Rogers GC.
europepmc +2 more sources