Results 71 to 80 of about 124,540 (297)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Rate Constants for Anion Transport by Steroid-Based Synthetic Anion Transporters [PDF]

Biophysical Journal, 2012
Cholapods are steroid-based synthetic anion transporters that bind anions with high affinity and promote their efflux from liposomes. To understand better the mechanism of action of cholapods, we evaluated the rate constants for anion transport by the cholapods AS09, LJ09 and TL145 using the method developed by Lauger (Science.
Li, Hongyu, Magro, Germinal, Judd, Luke W., Brotherhood, Peter R., Sheppard, David N., Davis, Anthony P.   +5 more
openaire   +1 more source

The Role of the Organic Moiety in the Diffusion and Transport of Carboxylates into Liposomes

Molecules
Understanding carboxylate transport through lipid membranes under physiological conditions is critical in biomedicine and biotechnology, as it allows for the emulation of biological membrane functions and can enhance the absorption of hydrophobic ...
Aaron Torres-Huerta, Hennie Valkenier
doaj   +1 more source

The therapeutic opportunities and pitfalls of using iron ion chelators to treat neurodegenerative diseases

FEBS Letters, EarlyView.
Although too much iron in the brain promotes neurodegeneration, iron ion chelators have had mixed effects in clinical trials. This review explains why; some chelators do not render the iron redox‐inactive (e.g., L1) whereas others do (e.g., desferrioxamine).
Barry Halliwell
wiley   +1 more source

Effect of a bound anion on the structure and dynamics of halorhodopsin from Natronomonas pharaonis

Structural Dynamics, 2019
Active ion transport across membranes is vital to maintaining the electrochemical gradients of ions in cells and is mediated by transmembrane proteins. Halorhodopsin (HR) functions as a light-driven inward pump for chloride ions. The protein contains all-
Misao Mizuno, Yumi Shimoo, Hideki Kandori, Yasuhisa Mizutani   +3 more
doaj   +1 more source

Sulphate and Chloride-Dependent Potassium Transport in Human Erythrocytes are Affected by Crude Venom from Nematocysts of the Jellyfish Pelagia noctiluca

Cellular Physiology and Biochemistry, 2013
Background: It has been reported that biologically active compounds extracted from Cnidaria venom may induce damage by oxidative stress. Erythrocytes are constantly exposed to oxidative stresses, which can contribute to sulphydril (SH-) group oxidation ...
Rossana Morabito, Angela Marino, Pietro Romano, Caterina Rigano, Giuseppa La Spada   +4 more
doaj   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

High selectivity framework polymer membranes chemically tuned towards fast anion conduction

Nature Communications
Studying ion transport in the interaction confinement regime has important implications for membrane design and advanced electrochemical devices. A key example is the rapid-charging capability of aqueous organic redox flow batteries, enabled by near ...
Junkai Fang, Guozhen Zhang, Marc-Antoni Goulet, Peipei Zuo, Yu Zhou, Hui Li, Jun Jiang, Michael D. Guiver, Zhengjin Yang, Tongwen Xu   +9 more
doaj   +1 more source

Investigation of ion transport and water content properties in anion exchange membranes based on polysulfone for solid alkaline fuel cell application [PDF]

Hydrogen, Fuel Cell & Energy Storage, 2015
In present research work, homogeneous anion exchange membranes based on polysulfone (QAPSFs) were prepared via chloromethylation, amination and alkalization.
Mona Iravaninia, Saba Azizi, Soosan Rowshanzamir   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source