Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies [PDF]
Bioconjugate Chemistry, 2019 Recognition of carbohydrates by antibodies can be affected by antigen composition and density. This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.
Cristina Costa, Tomaso Bottio
exaly +8 more sources
A general and accessible approach to enrichment and characterisation of natural anti-Neu5Gc antibodies from human samples [PDF]
RSC Chemical BiologyA workflow to enrich and detect anti-Neu5Gc antibodies from individual plasma specimens, using CMAH-transfected human cells as a source of Neu5Gc-containing glycans.
Craig Robson +2 more
exaly +6 more sources
At least two Fc Neu5Gc residues of monoclonal antibodies are required for binding to anti-Neu5Gc antibody [PDF]
Scientific Reports, 2016 AbstractTwo non-human glycan epitopes, galactose-α-1,3-galactose (α-gal) and Neu5Gc-α-2-6-galactose (Neu5Gc) have been shown to be antigenic when attached to Fab oligosaccharides of monoclonal antibodies (mAbs) , while α-gal attached to Fc glycans was not.
Junzhi Wang +2 more
exaly +6 more sources
Brain-dead humans as preclinical reference models for xenotransfusion: bridging nonhuman primates and clinical applications through in vitro evaluation [PDF]
Frontiers in PhysiologyBackgroundXenotransfusion using genetically engineered (GE) pig red blood cells (RBCs) offers a promising solution to blood shortages, particularly in emergency settings.
Tao Li +12 more
doaj +2 more sources
Increased Concentration of Anti-Egg Albumin Antibodies in Cerebrospinal Fluid and Serum of Patients with Alzheimer’s Disease—Discussion on Human Serpins’ Similarity and Probable Involvement in the Disease Mechanism [PDF]
BiomoleculesAlzheimer’s Disease (AD) is a multifactorial process. Amyloid plaque formation constitutes the main characteristic of the disease. Despite the identification of numerous factors associated with AD, the mechanism remains unclear in several aspects ...
Dionysia Amanatidou +11 more
doaj +2 more sources
MoleculesN-glycolylneuraminic acid (Neu5Gc), a sialic acid predominantly found in the non-neurohumoral fluids of hind-mouthed animals, is incapable of synthesizing Neu5Gc due to a deletion in the CMAH exon of the gene encoding human CMP-Neu5Gc hydroxylase.
Yuxi Guo +11 more
doaj +3 more sources
Expression of Human Thrombomodulin Prevents Early Thrombocytopenia and Thrombotic Microangiopathy in Pig-to-Nonhuman Primate Orthotopic Liver Xenotransplantation. [PDF]
XenotransplantationABSTRACT Background Severe thrombocytopenia and coagulation dysregulation remain major barriers to survival in pig‐to‐primate liver xenotransplantation models. Methods Porcine livers with five genetic modifications (GTKO, CMAHKO, β4GalNT2KO, hCD55, and hTBM) were orthotopically transplanted into four Tibetan macaques under two conventional ...
Zhang Z +17 more
europepmc +2 more sources
Antibody-Targeted Artificial T Cell and Natural Killer Cell Derived Vesicles for Cancer Immunotherapy. [PDF]
J Extracell VesiclesABSTRACT T cell and NK92 cell‐derived extracellular vesicles or artificial cell‐derived vesicles (ACDVs) exhibit anti‐cancer effects through inherited cytotoxic proteins like perforin and granzyme or transmembrane receptors like FasL and TRAIL. The anti‐cancer potential of artificial T and NK vesicles has been improved by attaching targeting moieties ...
Parlekar B +7 more
europepmc +2 more sources
Prevalence of anti-Neu5Gc antibodies in patients with hypothyroidism. [PDF]
Biomed Res Int, 2014 Background. N-Glycolylneuraminic acid (Neu5Gc) is a sialic acid synthesized by animals, but not by humans or birds. However, it can be incorporated in human cells and can trigger immune response. In the present study, we detected anti-Neu5Gc antibodies in samples of the general population and of patients suffering from hypothyroidism/Hashimoto’s ...
Eleftheriou P +9 more
europepmc +4 more sources
Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay. [PDF]
J Vis Exp, 2017 Cells are covered with a cloak of carbohydrate chains (glycans) that is commonly altered in cancer and that includes variations in sialic acid (Sia) expression. These are acidic sugars that have a 9-carbon backbone and that cap vertebrate glycans on cell surfaces.
Leviatan Ben-Arye S +3 more
europepmc +6 more sources