Presentation Mode of Glycans Affect Recognition of Human Serum anti-Neu5Gc IgG Antibodies [PDF]
Bioconjugate Chemistry, 2019 Recognition of carbohydrates by antibodies can be affected by antigen composition and density. This had been investigated in a variety of controllable multivalent systems using synthetic carbohydrate antigens, yet such effects on anticarbohydrate antibodies in circulating human serum have not been fully addressed thus far.
Cristina Costa, Tomaso Bottio
exaly +10 more sources
At least two Fc Neu5Gc residues of monoclonal antibodies are required for binding to anti-Neu5Gc antibody [PDF]
Scientific Reports, 2016 AbstractTwo non-human glycan epitopes, galactose-α-1,3-galactose (α-gal) and Neu5Gc-α-2-6-galactose (Neu5Gc) have been shown to be antigenic when attached to Fab oligosaccharides of monoclonal antibodies (mAbs) , while α-gal attached to Fc glycans was not.
Mark Wormald, Chuanfei Yu
exaly +8 more sources
A general and accessible approach to enrichment and characterisation of natural anti-Neu5Gc antibodies from human samples [PDF]
RSC Chemical BiologyA workflow to enrich and detect anti-Neu5Gc antibodies from individual plasma specimens, using CMAH-transfected human cells as a source of Neu5Gc-containing glycans.
Craig Robson +2 more
exaly +6 more sources
Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay. [PDF]
J Vis Exp, 2017 Cells are covered with a cloak of carbohydrate chains (glycans) that is commonly altered in cancer and that includes variations in sialic acid (Sia) expression. These are acidic sugars that have a 9-carbon backbone and that cap vertebrate glycans on cell surfaces.
Leviatan Ben-Arye S +3 more
europepmc +8 more sources
Anti-Gal and Anti-Neu5Gc Responses in Nonimmunosuppressed Patients After Treatment With Rabbit Antithymocyte Polyclonal IgGs. [PDF]
Transplantation, 2017 Polyclonal antihuman thymocyte rabbit IgGs (antithymocyte globulin [ATG]) are popular immunosuppressive drugs used to prevent or treat organ or bone-marrow allograft rejection, graft versus host disease, and autoimmune diseases. However, animal-derived glycoproteins are also strongly immunogenic and rabbit ATG induces serum sickness disease in almost ...
Salama A +11 more
europepmc +5 more sources
Potential deleterious role of anti‐Neu5Gc antibodies in xenotransplantation
Xenotransplantation, 2014 AbstractHuman beings do not synthesize the glycolyl form of the sialic acid (Neu5Gc) and only express the acetylated form of the sugar, whereas a diet‐based intake of Neu5Gc provokes a natural immunization and production of anti‐Neu5Gc antibodies in human serum.
Apolline Salama +3 more
openaire +5 more sources
Anti-Neu5Gc Antibody Kit Protocols v1
, 2016 Anti-Neu5Gc Antibody Kit Protocols for Flow Cytometry, ELISA, and Western blot.
Kelsey Miller
openaire +2 more sources
Anti-Neu5Gc Antibody Kit Protocol - Western Blot v2
, 2017 The Anti-Neu5Gc Antibody Kit contains the essential monospecific polyclonal chicken IgY antibody, along with a negative control primary antibody to detect the presence of Neu5Gc on glycoconjugates by Western blot (WB). Samples to be evaluated are first subjected to SDS-PAGE, followed by transfer to a nitrocellulose or polyvinylidenedifluoride (PVDF ...
Kelsey Miller
openaire +2 more sources
Anti-Neu5Gc Antibody Kit Protocol - Western blot v1
, 2016 The Anti-Neu5Gc Antibody Kit contains the essential monospecific polyclonal chicken IgY antibody, along with a negative control primary antibody to detect the presence of Neu5Gc on glycoconjugates by Western blot (WB). Samples to be evaluated are first subjected to SDS-PAGE, followed by transfer to a nitrocellulose or polyvinylidenedifluoride (PVDF ...
Kelsey Miller
openaire +2 more sources
Association between Neu5Gc carbohydrate and serum antibodies against it provides the molecular link to cancer: French NutriNet-Santé study [PDF]
BMC Medicine, 2020 Background High consumption of red and processed meat is commonly associated with increased cancer risk, particularly colorectal cancer. Antibodies against the red meat-derived carbohydrate N-glycolylneuraminic acid (Neu5Gc) exacerbate cancer in “human ...
Salam Bashir +18 more
doaj +2 more sources