Results 51 to 60 of about 2,678,192 (313)

Development of antibodies against severe fever with thrombocytopenia syndrome virus nucleoprotein for diagnosis

Applied Microbiology and Biotechnology
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral infectious disease caused by a novel Bandavirus in the family Phenuiviridae. The SFTS virus (SFTSV) is transmitted to various hosts, including humans, through tick bites, leading to ...
Eun-Mee Park, Seheon Kim, Seoyoen Lim, Parastou Rahimizadeh, Hyeyoon Jeon, Hyung Jin Lim, Sungjin Lee, Yong Bhum Song   +7 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Antibody production in transgenic plants

, 2022
S.301-318Antibodies bind with great affinity and specificity to their target antigens, allowing them to be exploited in research, medicine, agriculture, and industry.
Christou, P., Twyman, R.M., Fischer, R., Stoger, E., Schillberg, S.   +4 more
core   +1 more source

TGF-β2 increases eHSP90α secretion via upregulating secretory autophagy pathway

Cell Communication and Signaling
Background Extracellular heat shock protein 90α (eHSP90α) regulates diverse cellular processes such as wound healing, tumor metastasis, angiogenesis and cell differentiation etc.
Jing Li, Junmin Li, Tong Xie, Shuxin Huang, Haijiao Tian, Mengyun Liu, Xiumei Kong, Dongzhe Zhang, Khan Iqbal Nawaz, Mingli Wang, Hui Li, Jing Zhang, Fengling Yuan, Weikai Guo, Xiukun Cui, Hongmei Mu, Yanzhong Hu   +16 more
doaj   +1 more source

A novel one-step approach for the construction of yeast surface display Fab antibody libraries

Microbial Cell Factories, 2018
Background Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as ...
Simon Rosowski, Stefan Becker, Lars Toleikis, Bernhard Valldorf, Julius Grzeschik, Deniz Demir, Iris Willenbücher, Ramona Gaa, Harald Kolmar, Stefan Zielonka, Simon Krah   +10 more
doaj   +1 more source

Fc-Based Recombinant Henipavirus Vaccines Elicit Broad Neutralizing Antibody Responses in Mice

Viruses, 2020
The genus Henipavirus (HNVs) includes two fatal viruses, namely Nipah virus (NiV) and Hendra virus (HeV). Since 1994, NiV and HeV have been endemic to the Asia–Pacific region and responsible for more than 600 cases of infections. Two emerging HNVs, Ghana
Yaohui Li, Ruihua Li, Meirong Wang, Yujiao Liu, Ying Yin, Xiaodong Zai, Xiaohong Song, Yi Chen, Junjie Xu, Wei Chen   +9 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Antibody-Drug Conjugates for Cancer Therapy

, 2023
Antibody-drug conjugates (ADCs) take advantage of the specificity of a monoclonal antibody to deliver a linked cytotoxic agent directly into a tumour cell.
Sagun Parakh (9493061), Adam Parslow (9493046), Andrew Scott (9483443), Fook-Thean Lee (9491234), Hui Gan (9477872)   +4 more
core   +2 more sources

i-shaped antibody engineering enables conformational tuning of biotherapeutic receptor agonists

Nature Communications
The ability to leverage antibodies to agonize disease relevant biological pathways has tremendous potential for clinical investigation. Yet while antibodies have been successful as antagonists, immune mediators, and targeting agents, they are not readily
Matthew G. Romei, Brandon Leonard, Zachary B. Katz, Daniel Le, Yanli Yang, Eric S. Day, Christopher W. Koo, Preeti Sharma, Jack Bevers III, Ingrid Kim, Huiguang Dai, Farzam Farahi, May Lin, Andrey S. Shaw, Gerald Nakamura, Jonathan T. Sockolosky, Greg A. Lazar   +16 more
doaj   +1 more source

Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences

mAbs, 2020
The ability to genetically encode non-natural amino acids (nnAAs) into proteins offers an expanded tool set for protein engineering. nnAAs containing unique functional moieties have enabled the study of post-translational modifications, protein ...
Gargi Roy, Jason Reier, Andrew Garcia, Tom Martin, Megan Rice, Jihong Wang, Meagan Prophet, Ronald Christie, William Dall’Acqua, Sanjeev Ahuja, Michael A Bowen, Marcello Marelli   +11 more
doaj   +1 more source