Results 241 to 250 of about 751,456 (299)
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The Journal of Immunology, 1974
Abstract Rabbit antibody of restricted heterogeneity induced by a lactoside determinant has been specifically purified and further fractionated into functionally homogeneous populations. These preparations have been used to evaluate the association constants for binding to the bacteriophage φX174 to which had been coupled multiple ...
P V Gopalakrishnan, Fred Karush
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Abstract Rabbit antibody of restricted heterogeneity induced by a lactoside determinant has been specifically purified and further fractionated into functionally homogeneous populations. These preparations have been used to evaluate the association constants for binding to the bacteriophage φX174 to which had been coupled multiple ...
P V Gopalakrishnan, Fred Karush
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Determination of Antibody Affinity
Journal of Immunoassay, 2000(2000). Determination of Antibody Affinity. Journal of Immunoassay: Vol. 21, No. 2-3, pp. 211-234.
M H, van Regenmortel, A, Azimzadeh
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Antibody Purification Using Affinity Chromatography
2022Antibodies are an integral part of many biological assays and biotherapeutics. However, the sources from which antibodies are derived frequently contain other contaminants which may interfere with assays or cause adverse reactions if administered in vivo.
Arabelle, Cassedy, Richard, O'Kennedy
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Hypermutation in antibody affinity maturation
Current Opinion in Immunology, 1999By studying the role of mismatch repair in hypermutation at the immunoglobulin loci, the field of antibody hypermutation has been integrated into the larger area of DNA repair. Trans-acting factors - Ku70, Ku80 and possibly SWAP-70 - have been identified for the temporally related but not mechanistically related immunoglobulin heavy-chain class-switch.
M, Wabl, M, Cascalho, C, Steinberg
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Peptide Affinity Purification of Antibodies
Cold Spring Harbor Protocols, 2019This protocol describes antibody purification using a peptide affinity column. Peptides can be designed that use naturally occurring cysteines within the protein target's primary sequence, or a cysteine can be added to either end of the peptide to provide free thiols for attachment.
Jordan B, Fishman, Eric A, Berg
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Engineering antibody affinity and specificity
International Journal of Cancer, 1988A combination of ab initio calculations, "knowledge-based prediction", molecular graphics and site-directed mutagenesis has enabled us to probe the molecular details of antibody:antigen recognition and binding and to alter the affinity and specificity of an antibody for its antigen.
D M, Webster +4 more
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Affinity Purification of Antibodies
2015Antibodies are provided in a variety of formats that include antiserum, hybridoma culture supernatant, or ascites. They can all be used successfully in crude form for the detection of target antigens by immunoassay. However, it is advantageous to use purified antibody in defined quantity to facilitate assay reproducibility, economy, and reduced ...
Robert M, Hnasko, Jeffery A, McGarvey
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Affinity-bound antibody–drug conjugates
Nature Biomedical Engineering, 2019Computationally designed antibody–drug conjugates easily self-assemble via affinity ligands that non-covalently link a therapeutic drug to conserved sites on native IgG antibodies.
Christine S. Nervig, Shawn C. Owen
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Antibody affinity measurements
1997Abstract In order to assess the biological activity of antibody (Ab) molecules, it is necessary to measure the strength of their interaction with the antigen (Ag) in terms of the equilibrium constant KA (1). This constant corresponds to the inverse of the Ab concentration which is needed to obtain 50% of complexed species.
Hélène Saunal +2 more
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Affinity Chromatography for Antibody Purification
2014The availability of purified antibodies is prerequisite for many applications and the appropriate choice(s) of antibody-purification steps is crucial. Numerous methods have been developed for the purification of antibodies; however, affinity chromatography-based methods are the most extensively utilized.
Sushrut, Arora +2 more
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