Results 261 to 270 of about 526,913 (304)

Antibodies with Infinite Affinity:  Origins and Applications

Accounts of Chemical Research, 2006
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Nathaniel G, Butlin, Claude F, Meares
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Distribution of antibody affinities—II. Fractionation of antibody with respect to its hapten binding affinity

Immunochemistry, 1974
Abstract Antibodies from individual sera were fractionated on the basis of their binding affinity by use of a specific immunoadsorbent. The distribution of affinities in the original serum and in each of the series of 10 fractions derived from it was computed.
Y T, Kim, T P, Werblin, G W, Siskind
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Affinity screening for weak monoclonal antibodies

Journal of Immunological Methods, 1998
When selecting for monoclonal antibodies of a desired affinity, affinity chromatography can be a feasible alternative. This is of particular interest when low affinity monoclonal antibodies (dissociation constant (Kd) > 10(-4) M) are screened, as they are not easily recognised by traditional immunoassay procedures.
L, Leickt, A, Grubb, S, Ohlson
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Affinity of Antibody at A Secretory Site in The Rat

Immunological Investigations, 1985
Sprague-Dawley rats were administered DNP-BGG on day one of pregnancy by either local mammary gland vicinity injection or by gastric intubation. All rats later received local mammary gland vicinity injection of DNP-BGG on day 11 of pregnancy. Milk and serum were collected post-parturition. IgA and IgG antibody was separated in the milk and IgG antibody
D S, Cox, S, Furman, D, Muench
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Isolation of antigens and antibodies by affinity chromatography

Journal of Chromatography B, 2003
Antibody-antigen binding constants are commonly strong enough for an effective affinity purification of antibodies (by immobilized antigens) or antigens (by immobilized antibodies) to work out a straightforward purification method. A drawback is that antibodies are large protein molecules and subject to denaturation under conditions required for the ...
Vladimir I, Muronetz, Timo, Korpela
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Affinity-labeling of anti-lactose antibody

Immunochemistry, 1975
Abstract Purified anti-lactose antibodies induced in the horse and rabbit with a bacterial vaccine have been subjected to affinity-labeling with two bromoacetyl derivatives of lactose-containing ligands. The synthesis of these new radioactive reagents is described and the optimum conditions for the labeling reaction given.
P V, Gopalakrishnan, F, Karush
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Antibody affinity measurements

1997
Abstract In order to assess the biological activity of antibody (Ab) molecules, it is necessary to measure the strength of their interaction with the antigen (Ag) in terms of the equilibrium constant KA (1). This constant corresponds to the inverse of the Ab concentration which is needed to obtain 50% of complexed species.
Hélène Saunal, Robert Karlsson,, March. V Van Regenmortel   +2 more
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Determination by ellipsometry of the affinity of monoclonal antibodies

Journal of Immunological Methods, 1986
The reaction between monoclonal antibodies and surface-immobilised hapten was studied by ellipsometry, a method allowing absolute measurement of the surface concentration of proteins. Monoclonal antibodies against 2-phenyloxazolone were used and their affinity for the antigen in solution was determined by calculations of the equilibrium constant from ...
H, Nygren, M, Kaartinen, M, Stenberg
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Measurement of the Affinity of Antiviral Antibodies

1988
Publisher Summary This chapter describes various methods that are used to measure absolute and relative affinities, to pinpoint the difficulties and errors inherent in some of them, and to attempt to rationalize their solution. The heterogeneous nature of antibodies in antisera makes meaningful measurements of affinity very difficult.
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Affinity of tetanus toxoid antibody in glomerulonephritis

Clinical Immunology and Immunopathology, 1982
Abstract Properties which determine whether circulating immune complexes are nephritogenic in man could be influenced by immunological factors affecting antigen antibody ratios, size of complexes, and other characteristics of antibody production. There are conflicting reports on effects of antibody affinity on the development of glomerulonephritis in
R S, Walls, P, Godfrey, R C, Newland, G, Carney, J R, Lawrence   +4 more
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