Results 251 to 260 of about 526,913 (304)
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The Journal of Immunology, 1974
Abstract Rabbit antibody of restricted heterogeneity induced by a lactoside determinant has been specifically purified and further fractionated into functionally homogeneous populations. These preparations have been used to evaluate the association constants for binding to the bacteriophage φX174 to which had been coupled multiple ...
P V Gopalakrishnan, Fred Karush
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Abstract Rabbit antibody of restricted heterogeneity induced by a lactoside determinant has been specifically purified and further fractionated into functionally homogeneous populations. These preparations have been used to evaluate the association constants for binding to the bacteriophage φX174 to which had been coupled multiple ...
P V Gopalakrishnan, Fred Karush
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Peptide Affinity Purification of Antibodies
Cold Spring Harbor Protocols, 2019This protocol describes antibody purification using a peptide affinity column. Peptides can be designed that use naturally occurring cysteines within the protein target's primary sequence, or a cysteine can be added to either end of the peptide to provide free thiols for attachment.
Jordan B, Fishman, Eric A, Berg
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Affinity Purification of Antibodies
2015Antibodies are provided in a variety of formats that include antiserum, hybridoma culture supernatant, or ascites. They can all be used successfully in crude form for the detection of target antigens by immunoassay. However, it is advantageous to use purified antibody in defined quantity to facilitate assay reproducibility, economy, and reduced ...
Robert M, Hnasko, Jeffery A, McGarvey
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Hypermutation in antibody affinity maturation
Current Opinion in Immunology, 1999By studying the role of mismatch repair in hypermutation at the immunoglobulin loci, the field of antibody hypermutation has been integrated into the larger area of DNA repair. Trans-acting factors - Ku70, Ku80 and possibly SWAP-70 - have been identified for the temporally related but not mechanistically related immunoglobulin heavy-chain class-switch.
M, Wabl, M, Cascalho, C, Steinberg
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Purification of antibodies by affinity chromatography
Journal of Biochemical and Biophysical Methods, 2002This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. Affinity techniques allow antibody purification both in a single step chromatographic procedure as well as in complex purification protocols depending on the intention to use the target antibody.
Klaus, Huse +2 more
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Affinity of IgM and IgG antibodies
Immunochemistry, 1970Abstract Immunoglobulins M and G were prepared from a single bleeding from individual rabbits after an injection of a hapten-protein conjugate (nitro-iodo-oxyphenyl acetic acid and chicken globulin, NIP11CG). The concentration of anti-NIP in the preparations was determined by quantitative precipitation.
O, Mäkelä +2 more
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Engineering antibody affinity and specificity
International Journal of Cancer, 1988A combination of ab initio calculations, "knowledge-based prediction", molecular graphics and site-directed mutagenesis has enabled us to probe the molecular details of antibody:antigen recognition and binding and to alter the affinity and specificity of an antibody for its antigen.
D M, Webster +4 more
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Affinity Chromatography for Antibody Purification
2014The availability of purified antibodies is prerequisite for many applications and the appropriate choice(s) of antibody-purification steps is crucial. Numerous methods have been developed for the purification of antibodies; however, affinity chromatography-based methods are the most extensively utilized.
Sushrut, Arora +2 more
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The modification of affinity labeled antibodies
Immunochemistry, 1973Abstract We previously showed that antibodies which had been affinity labeled at a tyrosyl residue with a diazonium labeling reagent could be further modified usefully. By reductively cleaving the azotyrosine bond, 3-aminotyrosine (3-NH2Tyr) was generated in situ, and this residue could then serve as an initiation point for further modifications ...
N, Hadler, H, Metzger
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The importance of antibody affinity in the performance of immunoassays for antibody
Journal of Immunological Methods, 1985(I) Introduction Over the last 25 years or so, a wide range of new antibody immunoassay techniques has been developed to add to the historically accepted methods such as precipitation, agglutination and complement fixation. These newer techniques involve the use of electrophoresis, fluorescence, radioactivity, laser nephelometry, solid-phase ...
M W, Steward, A M, Lew
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