Results 81 to 90 of about 526,913 (304)

Selection of functional human antibodies from retroviral display libraries

, 2004
Antibody library technology represents a powerful tool for the discovery and design of antibodies with high affinity and specificity for their targets.
Álvarez-Vallina, Luis, Christian J. Buchholz, Carsten Finger, Klaus Cichutek, Compte, Marta, Johannes H. Urban, Finger, Carsten, Urban, Johannes H., Richard M. Schneider, Schneider, Richard M., Cichutek, Klaus, Buchholz, Christian, Marta Compte   +12 more
core   +1 more source

Learned features of antibody-antigen binding affinity

, 2023
Defining predictors of antigen-binding affinity of antibodies is valuable for engineering therapeutic antibodies with high binding affinity to their targets.
Raman, Rahul, Sasisekharan, Ram, Clark, Thomas, Miller, Nathaniel L.   +3 more
core   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Reducing affinity as a strategy to boost immunomodulatory antibody agonism

, 2023
Antibody responses during infection and vaccination typically undergo affinity maturation to achieve high-affinity binding for efficient neutralization of pathogens1,2.
Yu, Xiaojie, James, Sonya, Cox, Kerry L., Cragg, Mark, Tews, Ivo, Penfold, Christine A., Kim, Jinny, Orr, Christian, Glennie, Martin J., Chan, H.T. Claude, Mockridge, C. Ian, Essex, Jonathan W., Inzhelevskaya, Tatyana   +12 more
core   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin.

PLoS ONE, 2014
The epidermal growth factor receptor 2, ERBB2, is a well-validated target for cancer diagnostics and therapy. Recent studies suggest that the over-expression of this receptor in various cancers might also be exploited for antibody-based payload delivery,
Johan Nilvebrant, Mikael Åstrand, Maria Georgieva-Kotseva, Mattias Björnmalm, John Löfblom, Sophia Hober   +5 more
doaj   +1 more source

Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA) [PDF]

, 2013
The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage
Shen, Duanwen, Shen Duanwen, Fei Xie (437662), Xie, Fei, Edwards W. Barry, W. Barry Edwards (437663), Shen, D, Duanwen Shen, W. Barry Edwards, Xie Fei, W Barry Edwards, Fei Xie, Edwards, WB, Duanwen Shen (437661), Xie, F, Edwards, W. Barry   +15 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Unveiling the affinity–stability relationship in anti-measles virus antibodies: a computational approach for hotspots prediction

Frontiers in Molecular Biosciences
Recent years have seen an uptick in the use of computational applications in antibody engineering. These tools have enhanced our ability to predict interactions with antigens and immunogenicity, facilitate humanization, and serve other critical functions.
Rimpa Paul, Rimpa Paul, Keisuke Kasahara, Jiei Sasaki, Jorge Fernández Pérez, Ryo Matsunaga, Ryo Matsunaga, Takao Hashiguchi, Daisuke Kuroda, Daisuke Kuroda, Daisuke Kuroda, Kouhei Tsumoto, Kouhei Tsumoto, Kouhei Tsumoto   +13 more
doaj   +1 more source