Results 91 to 100 of about 583,416 (296)

A Multidimensional Engineering Strategy Reprograms Microglia via Targeted and Sustained‐Release Extracellular Vesicles for Spinal Cord Injury Repair

Advanced Science, EarlyView.
A multifunctional EV‐based nanoplatform (Ang‐TEVs@Gel) was engineered via preconditioning, surface targeting, and ROS‐responsive hydrogel encapsulation to reprogram microglia. This system restored autophagy via miR‐664a‐3p/PIK3CA axis, cleared myelin debris, resolved neuroinflammation, and promoted axon remyelination, ultimately achieving robust motor ...
Wu Xiong, Minhao Liu, Mingming Zheng, Jizhou Jia, Ziyan Zhu, Guang Kong, Jie Liu, Juan Wang, Wenbo Li, Qingyuan Wang, Qian Zhu, Peiran Chan, Tao Qin, Jiayang Wu, Yongjie Zhang, Chunming Tang, Cong Li, Jin Fan   +17 more
wiley   +1 more source

Application and Single‐Cell Regulation Mechanism of Engineered EVs Combined With 4D‐Printed Hydrogel for Infected Burn Repair

Advanced Science, EarlyView.
4D bioprinted hydrogel loaded with FGF2‐modified extracellular vesicles (4D@F‐EVs) targets dermal fibroblasts and elevates local wound FGF2 levels via sustained release of F‐EVs. It activates PI3K‐Akt signaling, restores critical KGF+ fibroblast–KGFR+ keratinocyte crosstalk, enhances robust angiogenesis and collagen deposition to efficiently repair ...
Xiaomin Wang, Xiaoyan Li, Xiao Xu, Kelun Zhang, Fengjiao Yu, Zhen Shang, Nailong Pan, Junlin Lv, Yiwei Xu, Yan Tang, Liang Zhang, Xiaotong Li, Taoyi Wang, Jing Tao, Jiaxu Song, Mengyu Zhang, Xin Ma, Dan Han, Xiaoying Kong, Wenhua Xu   +19 more
wiley   +1 more source

Nucleoside‐Modified mRNA Encoding Alpha‐Galactosidase A Ameliorates Fabry Disease Phenotypes in Human IPSC‐Derived Cardiomyocytes

Advanced Science, EarlyView.
Human iPSC‐derived Fabry cardiomyocytes exhibited broad transcriptional dysregulation, apoptosis, mitochondrial dysfunction, impaired reactive oxygen species handling, altered contractility, and abnormal calcium transient decay, potentially mediated by phospholamban hyperphosphorylation.
Malte Juchem, Lea Oehlsen, Sedef Ersoy, Jia Li Ye, Natalie Weber, Elisa Mohr, Wilson Agyapong, Junqing Liu, Maximilian Fuchs, Ke Xiao, Christopher Jahn, Reto Eggenschwiler, Tobias Cantz, Julia Beimdiek, Falk F. R. Buettner, Theresia Kraft, Jan Hegermann, Ivonne J. Knorr, Lisa Ernst, Linda Feldbrügge, Resa Puffert, Kevin Schmidt, Christian Bär, Jeannine Hoepfner, Thomas Thum   +24 more
wiley   +1 more source

Analysis of antibody isotypes.

, 2016
Anti-Pvs48/45 sera showing positive reactivity to Pfs48/45 in ELISA and Western blotting (4 out of 5 from CFA group and 2 out of 5 from Montanide ISA-51 group) were individually tested to compare immunoglobulin isotypes. ELISA plates coated with Pvs48/45
Yi Cao (229829), Geetha P. Bansal (3149496), Kristen Merino (3149499), Nirbhay Kumar (113311)   +3 more
core   +1 more source

Evaluation of Immuno-Rolling Circle Amplification for Multiplex Detection and Profiling of Antigen-Specific Antibody Isotypes

, 2021
Antibody characterization is essential for understanding the immune system and development of diagnostics and therapeutics. Current technologies are mainly focusing on the detection of antigen-specific immunoglobulin G (IgG) using bulk singleplex ...
Geukens, Nick, Yeh, Shu-hao, Madaboosi, Narayanan, Breukers, Jolien, Horta, Sara, Madaboosi, Narayanan,, Langseth, Christoffer Mattsson, Nilsson, Mats,, Neumann, Felix,, Vanhoorelbeke, Karen, Nilsson, Mats, Lammertyn, Jeroen, Kangro, Kadri, Neumann, Felix, Mattsson Langseth, Christoffer,   +14 more
core   +1 more source

Loss of LCN2 Function Ameliorates Glucocorticoid‐Induced Muscle Atrophy via Remodeling the Extracellular Matrix

Advanced Science, EarlyView.
Glucocorticoids transcriptionally activate LCN2 expression via GR nuclear translocation. Secreted LCN2 binds MMP9 to degrade skeletal muscle ECM collagen, blocks integrin‐mediated mechanotransduction, bidirectionally disrupts muscle protein homeostasis, and reveals a novel target for steroid‐induced muscle atrophy.
Hongwei Shi, Xiaojing Hao, Yi Yan, Fengyang Li, Yaru Zhang, Zixin Li, Wenjuan Xie, Jiayin Lu, Xiaomao Luo, Yanjun Dong, Haidong Wang, Juan Wang   +11 more
wiley   +1 more source

Root‐Inspired Microneedle Patch With Interlocked Rigid‐Soft Architecture for High Tissue‐Adaptiveness

Advanced Science, EarlyView.
A root‐inspired microneedle array patch uses lattice‐based mechanical interlocking to unite rigid microneedles with a flexible substrate. The interlocked rigid–soft architecture improves interfacial bonding, supports stable adhesion on curved and wet tissues under dynamic motion, and provides a versatile platform for robust biointerfacing and future ...
Jongchan Lee, Yeongjun Song, Yonghyun Cho, Hyo In Kim, Dong‐Hyun Youn, Jinbong Park, Seungbeom Choi, Dohyun Kwon, Brian Jun Lee, Sangyul Baik   +9 more
wiley   +1 more source

Allergen-specific antibody levels.

, 2016
The release of functional Bet v 1-specific IgE was quantified by β-hexosaminidase release from rat basophil leukaemia cells (RBL) (A). Bet v 1-specific IgG1 (B) and IgG2a (C) antibody levels were measured by ELISA.
Mirjana Drinic (2696968), Johnnie Akgün (429144), Gerhard Loupal (316856), Michael Kundi (727003), Ursula Wiedermann (314076), Anja Joachim (414610), Angelika Wagner (316853), Irma Schabussova (316848)   +7 more
core   +1 more source

Klotho‐Derived Peptide 1 Protects against Acute Kidney Injury by Directly Targeting Mitochondrial ATAD3A

Advanced Science, EarlyView.
This study uncovers that KP1, a Klotho‐derived peptide, protects the kidney against acute kidney injury, a clinical syndrome with high morbidity and mortality. KP1 enters tubular epithelial cells via endocytosis, directly interacts with the mitochondrial ATAD3A/HIGD2A complex, thereby preventing cytochrome c release, and caspases activation, and ...
Xiaoyao Zhang, Shihui Lin, Tianyu Wu, Zhixin Zhang, Hong Zhou, Xue Hong, Youhua Liu   +6 more
wiley   +1 more source

R21/Matrix-M malaria vaccine drives diverse immune responses in pre-exposed adults: insights from a phase IIb controlled human malaria infection trial

Frontiers in Immunology
IntroductionThe recently licenced R21/Matrix-M vaccine induces a protective antibody response. In this study, we examined vaccine-induced responses in semi-immune adults in a controlled human malaria infection (CHMI) Phase IIb clinical trial ...
Elizabeth Kibwana, Caroline Bundi, Domtila Kimani, Lydia Nyamako, Kelvias Keter, Agnes Mutiso, Rodney Ogwang, Duncan Bellamy, Katerina Rapi, Amelia Bajer, Samuel Provstgaard-Morys, Lisa Stockdale, Olivia Munoz, Mehreen S. Datoo, Alison Lawrie, Fernando Ramos-Lopez, Rachel Roberts, Mainga Hamaluba, Mainga Hamaluba, Adrian V. S. Hill, Philip Bejon, Philip Bejon, Katie J. Ewer, Melissa Kapulu, Melissa Kapulu   +24 more
doaj   +1 more source