Radioimmunoprecipitation Assay for Australia Antigen, Antibody, and Antigen-Antibody Complexes
SummaryPurified Australia antigen [Au(1)], conjugated with 125I, was reacted with and quantitatively precipitated by human anti-Au(1) in the presence of heterologous antihuman IgG and “dilute” ammonium sulfate. The sensitivity of the devised radioim-munoprecipitation assay procedure proved to be up to 1000 times more sensitive for the detection of ...
J A, Coller +3 more
openaire +2 more sources
Antibodies for immunolabeling by light and electron microscopy : not for the faint hearted [PDF]
Reliable antibodies represent crucial tools in the arsenal of the cell biologist and using them to localize antigens for immunocytochemistry is one of their most important applications.
Griffiths, Gareth, Lucocq, John Milton
core +1 more source
The aim of this study was to evaluate the results of the determination of IgG and IgM against TORCH complex antigens obtained by using a new automatic random-access (RAD 120 – RADIM/SEAC) instrument based on FEIA method, in comparison with the results ...
Mauro Carcheri +9 more
doaj +1 more source
Neutralization of Diverse Human Cytomegalovirus Strains Conferred by Antibodies Targeting Viral gH/gL/pUL128-131 Pentameric Complex [PDF]
Human cytomegalovirus (HCMV) is the leading cause of congenital viral infection, and developing a prophylactic vaccine is of high priority to public health.
Adler, Stuart P. +19 more
core +3 more sources
The present study was conducted to predict T-cell epitopes of the antigen 85 complex capable of stimulating antibody generation by using immunoinformatics approaches.
YOGY SIMANJUNTAK
doaj +1 more source
Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus [PDF]
Merkel cell polyomavirus (MCV) has been recently described as the cause for most human Merkel cell carcinomas. MCV is similar to simian virus 40 (SV40) and encodes a nuclear large T (LT) oncoprotein that is usually mutated to eliminate viral replication ...
Ali +45 more
core +2 more sources
Vaccination of Cattle with the N Terminus of LppQ of Mycoplasma mycoides subsp. mycoides Results in Type III Immune Complex Disease upon Experimental Infection [PDF]
Contagious bovine pleuropneumonia (CBPP) is a serious respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides. Current vaccines against CBPP induce short-lived immunity and can cause severe postvaccine reactions. Previous studies have
Brown RD +7 more
core +3 more sources
Vaccine-induced immune thrombotic thrombocytopenia: Updates in pathobiology and diagnosis
Coronavirus disease 2019 (COVID-19) is a viral respiratory infection caused by the severe acute respiratory syndrome virus (SARS-CoV-2). Vaccines that protect against SARS-CoV-2 infection have been widely employed to reduce the incidence of symptomatic ...
Stefan D. Jevtic +10 more
doaj +1 more source
The structures of secretory and dimeric immunoglobulin A [PDF]
Secretory (S) Immunoglobulin (Ig) A is the predominant mucosal antibody, which binds pathogens and commensal microbes. SIgA is a polymeric antibody, typically containing two copies of IgA that assemble with one joining-chain (JC) to form dimeric (d) IgA ...
Haloi, Nandan +6 more
core
Background The current therapeutic antibodies and chimeric antigen receptor (CAR) T cells are capable of recognizing surface antigens, but not of intracellular proteins, thus limiting the target coverage for drug development.
Xin Liu +14 more
doaj +1 more source

