Results 201 to 210 of about 19,186 (244)

Artificial Nucleotide-containing Aptamers Used in Tumor Therapy

Chemical Research in Chinese Universities, 2019
The high pharmaceutical cost and multi-drug resistance in tumor therapeutic agents hinder the further application of chemotherapy in tumor therapy. Artificial modified nucleic acid aptamers have the advantages of high binding affinity, programmability, and easy synthesis.
Xinyuan Qin, Yuanye Su, Jie Tan, Quan Yuan   +3 more
openaire   +1 more source

Aptamer-Modified Micellar Electrokinetic Chromatography for the Enantioseparation of Nucleotides

Analytical Chemistry, 2009
In this paper, a new aptamer-based capillary electrophoresis (CE) method, which was able to separate the enantiomers of an anionic target (adenosine monophosphate, AMP) displaying the same electrophoretic mobility as that of the oligonucleotidic chiral selector, is reported.
Ruta, J., Perrier, Sandrine, Ravelet, C., Roy, B., Perigaud, C., Peyrin, E.   +5 more
openaire   +3 more sources

HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single-Nucleotide Resolution

2015
An RNA sample is usually heterogeneous. RNA heterogeneity refers to difference in length or size (i.e., number of nucleotides [nt]), sequence, or alternative but coexisting conformations. Separation and purification of RNA is generally required for investigating the structure and function of RNA, such as RNA catalysis and RNA structure determination by
Zhen, Huang, Chi-Yen, Lin, William, Jaremko, Li, Niu   +3 more
openaire   +2 more sources

Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

The Analyst, 2016
We developed a simple method for fluorescence detection of single-nucleotide differences using binary DNA probes that form a cholic-acid-binding aptamer upon hybridization with the target DNA.
Saori, Kamoto, Masumi, Hyuga, Teru, Kato
openaire   +2 more sources

De novo design of DNA aptamers that target okadaic acid (OA) by docking-then-assembling of single nucleotides

Biosensors and Bioelectronics, 2022
Okadaic acid (OA) is a diarrhetic shellfish poison widespread in ocean, so its detection is of great significance to seafood safety. Because of good sensitivity and low cost, biosensors using nucleic-acid aptamers as the recognition molecules are emerging as an important detection tool.
Menghua, Song, Yuanyuan, Li, Ruihua, Gao, Jianping, Liu, Qiang, Huang   +4 more
openaire   +2 more sources

Aptamer-Derived Peptide Inhibitors of Rho Guanine Nucleotide Exchange Factors

2013
Small G proteins of the Rho family and their activators the guanine nucleotide exchange factors (RhoGEFs) regulate essential cellular functions and their deregulation has been associated with an amazing variety of human disorders, including cancer, inflammation, vascular diseases, and mental retardation.
Susanne, Schmidt, Anne, Debant
openaire   +2 more sources

Structural optimization and anti-allergic activity of nucleotide aptamers target to Cε3-Cε4

Biochemical Pharmacology, 2019
Nucleic acid aptamers have shown a broad application prospect in basic research, clinical diagnosis and treatment, new drug development and various other fields. We have screened the DNA aptamer A1 and A2 target at Cε3-Cε4 with high affinity and specificity, another aptamer A8, no affinity with Cε3-Cε4 protein, was as a negative control in this study ...
Yanlei Yang, Lijian Zhao, Su Zhang, Zhenzhen Xu, Lifang Hao, Zhe Cui, Kewei Bi, Yanfen Zhang, Zhongcheng Liu   +8 more
openaire   +2 more sources

Wash-Free and High-Contrast Imaging of Single-Nucleotide Variation in Single Cells Using Transcription-Amplified Light-Up RNA Aptamer

Analytical Chemistry, 2023
Single-nucleotide variation (SNV) imaging can indicate cellular heterogeneity and spatial pattern, but it remains challenging to produce high-gain signal while also yielding single-nucleotide resolution. Herein, we developed a light-up strategy for visualizing SNVs based on transcription amplification, enabling wash-free and high-contrast imaging of ...
Xuhan Xia, Feng Li, Yong Zhang, Yulin Zhu, Sishuo Song, Guiping He, Jiaqi Zhang, Qiang He, Ruijie Deng   +8 more
openaire   +2 more sources

Fluorescence Anisotropy Analysis for Mapping Aptamer–Protein Interaction at the Single Nucleotide Level

Journal of the American Chemical Society, 2011
Structural characterization of aptamer-protein interactions is challenging and limited despite the tremendous applications of aptamers. Here we for the first time report a fluorescence anisotropy (FA) approach for mapping the interaction of an aptamer and its protein target at the single nucleotide level.
Dapeng, Zhang, Meiling, Lu, Hailin, Wang
openaire   +2 more sources

In silico screening of ssDNA aptamer against Escherichia coli O157:H7: A machine learning and the Pseudo K-tuple nucleotide composition based approach

Computational Biology and Chemistry, 2021
This study was planned to in silico screening of ssDNA aptamer against Escherichia coli O157:H7 by combination of machine learning and the PseKNC approach. For this, firstly a total numbers of 47 validated ssDNA aptamers as well as 498 random DNA sequences were considered as positive and negative training data respectively. The sequences then converted
Mokhtar, Nosrati, Jafar, Amani
openaire   +2 more sources