Results 211 to 220 of about 19,186 (244)

Spectral Tuning by a Single Nucleotide Controls the Fluorescence Properties of a Fluorogenic Aptamer

Biochemistry, 2019
Fluorogenic aptamers are genetically encoded RNA aptamers that bind and induce the fluorescence of otherwise nonfluorescent small molecule dyes. These RNA-fluorophore complexes can be highly fluorescent and useful for RNA visualization and genetically encoded biosensors.
Grigory S. Filonov, Wenjiao Song, Samie R. Jaffrey   +2 more
openaire   +2 more sources

Effect of 3′-end capping of aptamer with various 2′,4′-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers

Bioorganic & Medicinal Chemistry Letters, 2010
The capping of the 3'-ends of thrombin binding aptamers (TBAs) with bridged nucleotides increased the nuclease resistances and the stabilities in human serum. The binding abilities of the aptamers were not affected by the capping. The capping could be simply executed via a one step enzymatic process using 2',4'-bridged nucleoside 5'-triphosphate and ...
Yuuya, Kasahara, Shunsuke, Kitadume, Kunihiko, Morihiro, Masayasu, Kuwahara, Hiroaki, Ozaki, Hiroaki, Sawai, Takeshi, Imanishi, Satoshi, Obika   +7 more
openaire   +2 more sources

SNPs (single nucleotide polymorphisms) detection based on the formation of cholic-acid binding DNA aptamer

Nucleic Acids Symposium Series, 2005
Simple and rapid methods for the detection of single nucleotide polymorphisms (SNPs) are required to analyze the genetic information. We have previously isolated DNA aptamers that bind to cholic acid by an in vitro selection method. A common feature of the sequences of the aptamers was that they included a secondary structure, a three-way junction, in ...
Teru, Kato, Atsuko, Yuki, Kazuyoshi, Yano, Yoshiko, Arikawa, Isao, Karube   +4 more
openaire   +2 more sources

In Situ Live Cell Sensing of Multiple Nucleotides Exploiting DNA/RNA Aptamers and Graphene Oxide Nanosheets

Analytical Chemistry, 2013
Nucleotides, for example, adenosine-5'-triphosphate (ATP) and guanosine-5'-triphosphate (GTP), are primary energy resources for numerous reactions in organisms including microtubule assembly, insulin secretion, ion channel regulation, and so on. In order to advance our understanding of the production and consumption of nucleoside triphosphates, a ...
Ying, Wang, Zhaohui, Li, Thomas J, Weber, Dehong, Hu, Chiann-Tso, Lin, Jinghong, Li, Yuehe, Lin   +6 more
openaire   +2 more sources

Effects of single nucleotide changes on the binding and activity of RNA aptamers to human papillomavirus 16 E7 oncoprotein

Biochemical and Biophysical Research Communications, 2011
A virally-encoded oncoprotein (E7 from human papillomavirus 16, involved in the initiation of cell transformation) was the target for RNA aptamer development by the process of systematic evolution of ligands by exponential enrichment (SELEX). A number of aptamers were identified, one of which was shown to inhibit the interaction between E7 and its ...
Clare, Nicol, David H J, Bunka, G Eric, Blair, Nicola J, Stonehouse   +3 more
openaire   +2 more sources

A Stably Protonated Adenine Nucleotide with a Highly Shifted pK_a Value Stabilizes the Tertiary Structure of a GTP‐Binding RNA Aptamer

Angewandte Chemie International Edition, 2016
AbstractRNA tertiary structure motifs are stabilized by a wide variety of hydrogen‐bonding interactions. Protonated A and C nucleotides are normally not considered to be suitable building blocks for such motifs since their pKa values are far from physiological pH.
Antje C. Wolter, A. Katharina Weickhmann, Amir H. Nasiri, Katharina Hantke, Oliver Ohlenschläger, Christoph H. Wunderlich, Christoph Kreutz, Elke Duchardt‐Ferner, Jens Wöhnert   +8 more
openaire   +2 more sources

The auto-inhibitory state of Rho guanine nucleotide exchange factor ARHGEF5/TIM can be relieved by targeting its SH3 domain with rationally designed peptide aptamers

Biochimie, 2015
The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases.
Ping, He, De-Li, Tan, Hong-Xiang, Liu, Feng-Lin, Lv, Wei, Wu   +4 more
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Nucleotide Modification and Polymerase Engineering for Creating a Novel Class of Artificial Nucleic Acid Aptamers

2011
Enzymatic transcription and reverse transcription of artificial nucleic acids would be an important technique to allow the application of artificial nucleic acids to random screening methods of nucleic acids such as systematic evolution of ligands by exponential enrichment (SELEX), Non-SELEX selection, one-step selection etc.
Y. Imaizumi, Y. Takano, S. Kitadume, H. Ozaki, S. Obika, M. Kuwahara   +5 more
openaire   +1 more source

A Study on the Effect of an Aptamer with an Embedded Phosphate-Methylated Nucleotide on the Binding of a Target Molecule Using Molecular Simulation

2020
Uncharged DNA analogues are frequently used to be synthesized as oligomers or combined with nucleic acids to synthesize chimeric oligomers. Owing to their special structures and the uncharged property, the oligomers with DNA analogues are useful in many applications, like fluorescence in situ hybridization (FISH), biosensors, gene chips, etc.
Wen-Pin Hu, Hui-Ting Lin, Wen-Yih Chen, Jeffrey J. P. Tsai   +3 more
openaire   +1 more source

A Nucleotide‐Derived Fluorophore for Visualizing G‐Quadruplex Assembly and Introduction of Cation‐Regulated Activity into the Thrombin Binding Aptamer

ChemBioChem
Nucleic acid aptamers are artificial recognition elements with great potential in biotechnology. For their effective integration into nanodevices, rational strategies for optimizing aptamer affinity and regulating activity are essential. Artificial nucleotide analogs offer versatile tools for both fundamental and applied research in the aptamer field ...
Rugiya Alieva, Svetlana Sokolova, Ilya Oleynikov, Roman Novikov, Timofei Zatsepin, Andrey Aralov, Elena Zavyalova   +6 more
openaire   +1 more source