Results 231 to 240 of about 581,942 (266)

Signaling aptamers created using fluorescent nucleotide analogues.

Analytical Chemistry, 2006
A new approach to creating fluorescent signaling aptamers using fluorescent nucleotide analogues is presented. The fluorescence quantum yield of nucleotide analogues such as 2-aminopurine strongly depends on base stacking interactions when incorporated into double or single stranded DNA. This property is used to generate a binding-specific fluorescence
E. Katilius, Zivile Katiliene, N. Woodbury   +2 more
semanticscholar   +3 more sources

Artificial Nucleotide Aptamer-Based Field-Effect Transistor for Ultrasensitive Detection of Hepatoma Exosomes

Analytical Chemistry, 2022
An aptamer-based field-effect transistor (Apta-FET) is a well-developed assay method with high selectivity and sensitivity. Due to the limited information density that natural nucleotide library holds, the Apta-FET faces fundamental restriction in universality to detect various types of analytes.
Yiheng Chen, Derong Kong, Liping Qiu, Yungen Wu, Changhao Dai, Shi Luo, Zhipeng Huang, Qiuyuan Lin, Hui Chen, Sitao Xie, Li Geng, Jun Zhao, Weihong Tan, Yunqi Liu, Dacheng Wei   +14 more
openaire   +2 more sources

The auto-inhibitory state of Rho guanine nucleotide exchange factor ARHGEF5/TIM can be relieved by targeting its SH3 domain with rationally designed peptide aptamers.

Biochimie, 2015
The short isoform of Rho guanine nucleotide exchange factor ARHGEF5 is known as TIM, which plays diverse roles in, for example, tumorigenesis, neuronal development and Src-induced podosome formation through the activation of its substrates, the Rho family of GTPases.
P. He, Deli Tan, Hongxiang Liu, Feng-lin Lv, Wei Wu   +4 more
semanticscholar   +3 more sources

Effects of single nucleotide changes on the binding and activity of RNA aptamers to human papillomavirus 16 E7 oncoprotein.

Biochemical and Biophysical Research Communications, 2011
A virally-encoded oncoprotein (E7 from human papillomavirus 16, involved in the initiation of cell transformation) was the target for RNA aptamer development by the process of systematic evolution of ligands by exponential enrichment (SELEX). A number of aptamers were identified, one of which was shown to inhibit the interaction between E7 and its ...
Clare Nicol, D. Bunka, G. Blair, N. Stonehouse   +3 more
semanticscholar   +3 more sources

Aptamer-Modified Micellar Electrokinetic Chromatography for the Enantioseparation of Nucleotides

Analytical Chemistry, 2009
In this paper, a new aptamer-based capillary electrophoresis (CE) method, which was able to separate the enantiomers of an anionic target (adenosine monophosphate, AMP) displaying the same electrophoretic mobility as that of the oligonucleotidic chiral selector, is reported.
Ruta, J., Perrier, Sandrine, Ravelet, C., Roy, B., Perigaud, C., Peyrin, E.   +5 more
openaire   +3 more sources

Engineered Nucleotide Chemicapacitive Microsensor Array Augmented with Physics-Guided Machine Learning for High-Throughput Screening of Cannabidiol.

Small, 2022
The recent legalization of cannabidiol (CBD) to treat neurological conditions such as epilepsy has sparked rising interest across global pharmaceuticals and synthetic biology industries to engineer microbes for sustainable synthetic production of ...
S. H. Yap, Jieming Pan, Dao Viet Linh, XiangYu Zhang, Xinghua Wang, Wei Zhe Teo, Evgeny Zamburg, C. Tham, W. Yew, C. Poh, A. Thean   +10 more
semanticscholar   +1 more source

Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

The Analyst, 2016
We developed a simple method for fluorescence detection of single-nucleotide differences using binary DNA probes that form a cholic-acid-binding aptamer upon hybridization with the target DNA.
Saori, Kamoto, Masumi, Hyuga, Teru, Kato
openaire   +2 more sources

De novo design of DNA aptamers that target okadaic acid (OA) by docking-then-assembling of single nucleotides

Biosensors and Bioelectronics, 2022
Okadaic acid (OA) is a diarrhetic shellfish poison widespread in ocean, so its detection is of great significance to seafood safety. Because of good sensitivity and low cost, biosensors using nucleic-acid aptamers as the recognition molecules are emerging as an important detection tool.
Menghua, Song, Yuanyuan, Li, Ruihua, Gao, Jianping, Liu, Qiang, Huang   +4 more
openaire   +2 more sources

Direct Modeling of DNA and RNA Aptamers with AlphaFold 3: A Promising Tool for Predicting Aptamer Structures and Aptamer–Target Interactions

ACS Synthetic Biology
Aptamers, single-stranded nucleic acids that fold into unique three-dimensional shapes, bind selectively to non-nucleotide target molecules, making them promising ligands for diagnostic and therapeutic applications.
Steven Ochoa, V. Milam
semanticscholar   +1 more source

Aptamer-Derived Peptide Inhibitors of Rho Guanine Nucleotide Exchange Factors

2013
Small G proteins of the Rho family and their activators the guanine nucleotide exchange factors (RhoGEFs) regulate essential cellular functions and their deregulation has been associated with an amazing variety of human disorders, including cancer, inflammation, vascular diseases, and mental retardation.
Susanne, Schmidt, Anne, Debant
openaire   +2 more sources