Results 101 to 110 of about 29,754 (236)

Peptide Receptor‐Functionalized AuNP‐Embedded Zwitterionic Biopolymeric Nanohydrogel for Electrochemical Sclerostin Sensing

Advanced Science, EarlyView.
We report an electrochemical biosensor that employs biopanning‐derived affinity peptides as selective receptors on a multifunctional chitosan–zwitterionic–gold nanoparticle (CS–ZI–AuNPs) nanohydrogel interface. The CS–ZI–AuNPs nanohydrogel exhibits antifouling properties, abundant sites for stable peptide immobilization, and efficient electrochemical ...
Hyo Jeong Yang, Jae Hwan Shin, Mingi Jo, Changyeon Lee, Hyunmin Yi, Al‐Montaser Bellah H. Ali, Jong Pil Park   +6 more
wiley   +1 more source

Dissipatively Fueled Unidirectionally Communicating DNA Circuits That Control Biocatalysis

Angewandte Chemie, EarlyView.
Unidirectionally communicating, out‐of‐equilibrium DNA circuits are used to control enzyme activity. Transient DNAzyme activation generates fuel for temporal trypsin activation. Cyclic fuel‐driven hybridization and exonuclease‐mediated reset enable precise control over lifetimes, which is analyzed using combined experimental and computational ...
Philippe Jung, Daniel Felder, Gurudas Chakraborty, Tim Seifert, Matthias Wessling, Lifei Zheng, Andreas Herrmann   +6 more
wiley   +2 more sources

A Solution for Exosome‐Based Analysis: Surface‐Enhanced Raman Spectroscopy and Artificial Intelligence

Advanced Intelligent Discovery, EarlyView.
Exosomes are emerging as powerful biomarkers for disease diagnosis and monitoring. This review highlights the integration of surface‐enhanced Raman spectroscopy with artificial intelligence to enhance molecular fingerprinting of exosomes. Machine learning and deep learning techniques improve spectral interpretation, enabling accurate classification of ...
Munevver Akdeniz, Zakarya Al‐Shaebi, Omer Aydin   +2 more
wiley   +1 more source

Visualizing and Quantifying microRNA‐Induced DNA Origami Separation at the Nanoscale

Angewandte Chemie, EarlyView.
Clinically relevant miRNA biomarkers trigger the disassembly of DNA origami dimers into monomers through a toehold‐mediated strand displacement reaction. High‐speed AFM was used to visualize this reaction in real time, while solid‐state nanopore measurements quantified the populations of dimers and monomers, as well as the resulting miRNA concentration,
Chalmers C. C. Chau, Varun Gupta, George R. Heath, Christoph Wälti, Paolo Actis   +4 more
wiley   +2 more sources

Characterization of the Binding Properties of Ten Aptamers Using the Intrinsic Fluorescence of Oxytetracycline

ChemistryOpen
Tetracyclines are a class of commonly used four‐ringed antibiotics. A series of DNA aptamers were recently obtained using the capture‐SELEX (systematic evolution of ligands by exponential enrichment) method to bind to oxytetracycline, and one of the ...
Yichen Zhao, Biwen Gao, Juewen Liu
doaj   +1 more source

Machine Learning‐Assisted Infectious Disease Detection in Low‐Income Areas: Toward Rapid Triage of Dengue and Zika Virus Using Open‐Source Hardware

Advanced Intelligent Discovery, EarlyView.
This study introduces an affordable machine learning platform for simultaneous dengue and zika detection using fluorine‐doped tin oxide thin films modified with gold nanoparticles and DNA aptamers. Designed for low‐cost, hardware‐limited devices (< $25), the model achieves 95.3% accuracy and uses only 9.4 kB of RAM, demonstrating viability for resource‐
Marina Ribeiro Batistuti Sawazaki, Bassam Bachour Junior, Marcelo Mulato, José Ilton de Oliveira Filho   +3 more
wiley   +1 more source

The 10–23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions

Angewandte Chemie, EarlyView.
This review focuses on the 10–23 DNAzyme in diagnostics, spanning unregulated and regulated target‐recognition modes, and their functionalization across colorimetric, fluorescent, electrochemical, electrochemiluminescent, and intracellular biosensing strategies.
Connor Nurmi, Jake Brill, Sanne Roumans, Amal Mathai, John D. Brennan, Yingfu Li   +5 more
wiley   +2 more sources

Aptamers in Therapeutics

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2016
Aptamers are single strand DNA or RNA molecules, selected by an iterative process known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Due to various advantages of aptamers such as high temperature stability, animal free, cost effective production and its high affinity and selectivity for its target make them attractive ...
openaire   +3 more sources

Mucin Glycoprotein Nanoparticles Enable a Selective Antisense Therapy for Oncogenic MicroRNAs

Advanced NanoBiomed Research, EarlyView.
Mucin glycoproteins are turned into nanoparticles by employing synthetic DNA strands, which have a dual function: they stabilize the nanoparticles and act as binding sites for intracellular miRNA‐21. Thus, upon internalization into tumor cells, these mucin nanoparticles can deplete miRNA‐21 from the cytosol, which induces apoptosis in vitro and in vivo.
Ceren Kimna, Thomas Mayer, Di Zhang, Matthias Marczynski, Carolin A. Rickert, Fabio Henkel, Lennart J. K. Weiss, Friedrich C. Simmel, Yuxia Luan, Oliver Lieleg   +9 more
wiley   +1 more source

A Real‐Time Antifouling Multivalent Aptamer Platform for Wash‐Free Electrochemical Detection of Low‐Abundance Biomarkers in Human Plasma

Angewandte Chemie, EarlyView.
Detection of low‐abundance proteins at the point‐of‐care is key for early detection and real‐time monitoring. The clinical translation of such assays is hindered by biofouling, matrix variability, and signal instability. RT‐MagMAp assay overcomes these challenges and enables precise protein quantification in human plasma using a hierarchical assembly ...
Payel Sen, Survanshu Saxena, Zijie Zhang, Navreen Sandhu, Pranati Das, Jimmy Gu, Todd Hoare, Yingfu Li, Leyla Soleymani   +8 more
wiley   +2 more sources