The Arabidopsis ppi1 mutant is specifically defective in the expression, chloroplast import, and accumulation of photosynthetic proteins [PDF]
The import of nucleus-encoded proteins into chloroplasts is mediated by translocon complexes in the envelope membranes. A component of the translocon in the outer envelope membrane, Toc34, is encoded in Arabidopsis by two homologous genes, atTOC33 and ...
Baldwin, Amy +27 more
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In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots [PDF]
Background: Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome.
Sorieul Mathias +9 more
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Spatio-temporal expression patterns of Arabidopsis thaliana and Medicago truncatula defensin-like genes [PDF]
Plant genomes contain several hundred defensin-like (DEFL) genes that encode short cysteine-rich proteins resembling defensins, which are well known antimicrobial polypeptides.
Nallu, Sumitha +51 more
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Tonoplast intrinsic proteins and vacuolar identity [PDF]
TIPs (tonoplast intrinsic proteins) have been traditionally used as markers for vacuolar identity in a variety of plant species and tissues. In the present article, we review recent attempts to compile a detailed map of TIP expression in Arabidopsis, in ...
Gattolin, Stefano +2 more
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Characterization of the Arabidopsis Heterotrimeric G Protein [PDF]
We have used fluorescence resonance energy transfer and co-immunoprecipitation to analyze the interactions among the alpha, beta, and gamma1 subunits of the Arabidopsis heterotrimeric G protein. Using cyan and yellow fluorescent protein fusion constructs, we show that overexpressed Ggamma1 localizes to protoplast membranes, but Gbeta exhibits membrane ...
Shiyu, Wang +2 more
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Direct Modulation of Protein Level in Arabidopsis [PDF]
Dear Editor, The general way to probe functions of a protein in vivo is to perturb its level and then observe subsequent phenotypic changes.In plants,modulation of protein level is mainly carried out at DNA or RNA level,which is indirect and thus affected by stability of the target protein.Thus,experimental approaches to perturb protein level directly
Su, Lei +4 more
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The Arabidopsis Thylakoid Protein PAM68 Is Required for Efficient D1 Biogenesis and Photosystem II Assembly [PDF]
Photosystem II (PSII) is a multiprotein complex that functions as a light-driven water:plastoquinone oxidoreductase in photosynthesis. Assembly of PSII proceeds through a number of distinct intermediate states and requires auxiliary proteins.
Leister, D. +37 more
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The protein expression landscape of the Arabidopsis root [PDF]
Because proteins are the major functional components of cells, knowledge of their cellular localization is crucial to gaining an understanding of the biology of multicellular organisms. We have generated a protein expression map of the Arabidopsis root providing the identity and cell type-specific localization of ...
Petricka, Jalean J +10 more
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Functional specialization amongst the Arabidopsis Toc159 family of chloroplast protein import receptors [PDF]
The initial stages of preprotein import into chloroplasts are mediated by the receptor GTPase Toc159. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90.
Jocelyn Beédard +32 more
core +1 more source
Phosphorylation of plastoglobular proteins inArabidopsis thaliana [PDF]
Plastoglobules (PGs) are plastid lipid-protein particles with a small specialized proteome and metabolome. Among the 30 core PG proteins are six proteins of the ancient ABC1 atypical kinase (ABC1K) family and their locations in an Arabidopsis mRNA-based co-expression network suggested central regulatory roles.
Jens N. Lohscheider +2 more
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