Results 41 to 50 of about 80,337 (315)

CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection

open access: yesNature Communications, 2019
The S-layer is a proteinaceous envelope often found in bacterial and archaeal cells. Here, the authors use CRISPR-based technology to silence slaB, encoding the S-layer membrane anchor, to show that an intact S-layer is important for cell division and ...
Isabelle Anna Zink   +5 more
doaj   +1 more source

Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales

open access: yesBiomolecules, 2020
Prokaryotes are constantly coping with attacks by viruses in their natural environments and therefore have evolved an impressive array of defense systems.
Isabelle Anna Zink   +2 more
doaj   +1 more source

Analysis of Cell–Cell Bridges in Haloferax volcanii Using Electron Cryo-Tomography Reveal a Continuous Cytoplasm and S-Layer

open access: yesFrontiers in Microbiology, 2021
Halophilic archaea have been proposed to exchange DNA and proteins using a fusion-based mating mechanism. Scanning electron microscopy previously suggested that mating involves an intermediate state, where cells are connected by an intercellular bridge ...
Shamphavi Sivabalasarma   +7 more
doaj   +1 more source

The multidrug and toxin extrusion (MATE) transporter DTX51 antagonizes non‐cell‐autonomous HLS1–AMP1 signaling in a region‐specific manner

open access: yesFEBS Letters, EarlyView.
The Arabidopsis mutants hls1 hlh1 and amp1 lamp1 exhibit pleiotropic developmental phenotypes. Although the functions of the causative genes remain unclear, they act in the same genetic pathway and are thought to generate non‐cell‐autonomous signals.
Takashi Nobusawa, Makoto Kusaba
wiley   +1 more source

Photoactivatable Synthetic Exosomes for RNA‐Based Communication Between Artificial Cells and Living Cells

open access: yesAngewandte Chemie, EarlyView.
This article describes a modular strategy to construct artificial cells able to regulate mammalian cell protein expression via vesicle‐based RNA communication. Considering the important role of extracellular vesicles in genetic information transfer, this artificial exosome design is a significant advance toward mimicking nucleic acid communication ...
Alexander B. Cook   +4 more
wiley   +2 more sources

Lipidomic chemotaxonomy aligned with phylogeny of Halobacteria

open access: yesFrontiers in Microbiology, 2023
Archaea play an important role in global biogeochemical cycles and are considered ancestral to eukaryotes. The unique lipid composition of archaea, characterized by isoprenoid alkyl chains and ether linkage to glycerol-1-phosphate, offers valuable ...
Wenyong Yao   +11 more
doaj   +1 more source

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

open access: yesFEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

Discovery of fructans in Archaea [PDF]

open access: yesCarbohydrate Polymers, 2019
Fructans are fructose-based oligo- and polysaccharides derived from sucrose that occur in a plethora of Eubacteria and plants. While fructan-producing (fructanogenic) Eubacteria are abundant in hypersaline environments, fructan production by Archaea has never been reported before.
Kirtel, Onur   +3 more
openaire   +5 more sources

The nicotinamide hypothesis revisited—plant defense signaling integrating PARP, nicotinamide, nicotinic acid, epigenetics, and glutathione

open access: yesFEBS Letters, EarlyView.
Nicotinamide (NIC) and nicotinic acid (NIA) are proposed as stress signaling compounds in plants. Oxidative stress may lead to single strand breaks (SSB) in DNA, which activate poly(ADP‐ribose) polymerase (PARP). NIC and NIA are then formed from NAD. NIC and NIA can promote epigenetic changes leading to the expression of defense genes specific for the ...
Torkel Berglund, Anna B. Ohlsson
wiley   +1 more source

Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery

open access: yeseLife, 2017
The archaellum is the macromolecular machinery that Archaea use for propulsion or surface adhesion, enabling them to proliferate and invade new territories.
Bertram Daum   +7 more
doaj   +1 more source

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