Results 31 to 40 of about 102,839 (240)

Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2

open access: yesViruses, 2018
Genetic engineering of viruses has generally been challenging. This is also true for archaeal rod-shaped viruses, which carry linear double-stranded DNA genomes with hairpin ends.
David Mayo-Muñoz   +5 more
doaj   +1 more source

Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste [PDF]

open access: yes, 2016
: Inhibition by ammonium at concentrations above 1000 mgN/L is known to harm the methanogenesis phase of anaerobic digestion. We anaerobically digested swine waste and achieved steady state COD-removal efficiency of around 52% with no fatty-acid or H ...

core   +1 more source

The transcriptional regulator EarA and intergenic terminator sequences modulate archaellation in Pyrococcus furiosus

open access: yesFrontiers in Microbiology, 2023
The regulation of archaellation, the formation of archaeal-specific cell appendages called archaella, is crucial for the motility, adhesion, and survival of archaeal organisms.
Richard Stöckl   +5 more
doaj   +1 more source

Thermostable Bioluminescent Intercalating Dyes for Real‐Time, Integrated Nucleic Acid Amplification and Detection

open access: yesAngewandte Chemie, EarlyView.
We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter   +8 more
wiley   +2 more sources

Enzymatic Switching Between Archaeal DNA Polymerases Facilitates Abasic Site Bypass

open access: yesFrontiers in Microbiology, 2021
Abasic sites are among the most abundant DNA lesions encountered by cells. Their replication requires actions of specialized DNA polymerases. Herein, two archaeal specialized DNA polymerases were examined for their capability to perform translesion DNA ...
Xu Feng   +10 more
doaj   +1 more source

Protein acetylation in archaea, bacteria, and eukaryotes

open access: yes, 2010
Proteins can be acetylated at the alpha-amino group of the N-terminal amino acid (methionine or the penultimate amino acid after methionine removal) or at the epsilon-amino group of internal lysines.
Soppa, Jörg (Prof. Dr.)
core   +1 more source

Prefoldins in Archaea

open access: yes, 2018
Molecular chaperones promote the correct folding of proteins in aggregation-prone cellular environments by stabilizing nascent polypeptide chains and providing appropriate folding conditions. Prefoldins (PFDs) are molecular chaperones found in archaea and eukaryotes, generally characterized by a unique jellyfish-like hexameric structure consisting of a
Lim, S, Glover, DJ, Clark, DS
openaire   +3 more sources

ADP-dependent Phosphofructokinases in Mesophilic and Thermophilic Methanogenic Archaea

open access: yes, 2001
Phosphofructokinase (PFK) is a key enzyme of the glycolytic pathway in all domains of life. Two related PFKs, ATP-dependent and PPi-dependent PFK, have been distinguished in bacteria and eucarya, as well as in some archaea.
Kengen, S.W.M.   +7 more
core   +1 more source

Diversity and Spatial Distribution of Prokaryotic Communities Along A Sediment Vertical Profile of A Deep-Sea Mud Volcano Maria G. Pachiadaki & Argyri Kallionaki [PDF]

open access: yes, 2011
We investigated the top 30-cm sediment prokaryotic community structure in 5-cm spatial resolution, at an active site of the Amsterdam mud volcano, East Mediterranean Sea, based on the 16S rRNA gene diversity.
Anke Dählmann   +12 more
core   +1 more source

Putative Nucleotide-Based Second Messengers in the Archaeal Model Organisms Haloferax volcanii and Sulfolobus acidocaldarius

open access: yesFrontiers in Microbiology, 2021
Research on nucleotide-based second messengers began in 1956 with the discovery of cyclic adenosine monophosphate (3′,5′-cAMP) by Earl Wilbur Sutherland and his co-workers.
Frank Braun   +4 more
doaj   +1 more source

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