iScience, 2023 Summary: Single-stranded DNA-binding proteins (SSBs) have been regarded as indispensable replication factors. Herein, we report that the genes encoding the canonical SSB (SisSSB) and the non-canonical SSB (SisDBP) in Saccharolobus islandicus REY15A are ...
Yuanxi Xiao +12 more
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Regulated polyploidy in halophilic archaea [PDF]
, 2006 Polyploidy is common in higher eukaryotes, especially in plants, but it is generally assumed that most prokaryotes contain a single copy of a circular chromosome and are therefore monoploid.
Jörg Soppa +7 more
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Expanded Dataset Reveals the Emergence and Evolution of DNA Gyrase in Archaea [PDF]
, 2022 DNA gyrase is a type II topoisomerase with the unique capacity to introduce negative supercoiling in DNA. In bacteria, DNA gyrase has an essential role in the homeostatic regulation of supercoiling. While ubiquitous in bacteria, DNA gyrase was previously
Basta, Tamara +5 more
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CRISPR-Cas Adaptive Immune Systems of the Sulfolobales: Unravelling Their Complexity and Diversity
Life, 2015 The Sulfolobales have provided good model organisms for studying CRISPR-Cas systems of the crenarchaeal kingdom of the archaea. These organisms are infected by a wide range of exceptional archaea-specific viruses and conjugative plasmids, and their ...
Roger A. Garrett +11 more
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Hyperthermophilic methanogenic archaea act as high-pressure CH4 cell factories
Communications Biology, 2021 Mauerhofer et al. examine 80 species of methanogenic archaea at high pressures and evaluate growth and methane production, identifying Methanotorris igneus and Methanocaldococcoccus jannaschii as high-pressure methane cell factories.
Lisa-Maria Mauerhofer +9 more
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Nitrogen regulation of protein–protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea [PDF]
, 2013 Gene homologs of GlnK PII regulators and AmtB-type ammonium transporters are often paired on prokaryotic genomes, suggesting these proteins share an ancient functional relationship.
Soppa, Jörg +6 more
core +1 more source
Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2
Nature Communications, 2022 Single-molecule FRET measurements provide detailed insights into the conformational states and dynamics of human Argonaute 2 that are required for its function at the core of the eukaryotic RNA silencing pathway.
Sarah Willkomm +6 more
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Experimental characterization of Cis-acting elements important for translation and transcription in Halophilic Archaea [PDF]
, 2007 Summary The basal transcription apparatus of archaea is well characterized. However, much less is known about the mechanisms of transcription termination and translation initation.
Jörg Soppa +11 more
core +1 more source
Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2
Viruses, 2018 Genetic engineering of viruses has generally been challenging. This is also true for archaeal rod-shaped viruses, which carry linear double-stranded DNA genomes with hairpin ends.
David Mayo-Muñoz +5 more
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Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste [PDF]
, 2016 : Inhibition by ammonium at concentrations above 1000 mgN/L is known to harm the methanogenesis phase of anaerobic digestion. We anaerobically digested swine waste and achieved steady state COD-removal efficiency of around 52% with no fatty-acid or H ...
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