Results 51 to 60 of about 22,965 (136)

Accommodation of Helical Imperfections in Rhodobacter sphaeroides Argonaute Ternary Complexes with Guide RNA and Target DNA

Cell Reports, 2018
Summary: Prokaryotic Argonaute (Ago) proteins were recently shown to target foreign genetic elements, thus making them a perfect model for studies of interference mechanisms.
Yiwei Liu, Daria Esyunina, Ivan Olovnikov, Marianna Teplova, Andrey Kulbachinskiy, Alexei A. Aravin, Dinshaw J. Patel   +6 more
doaj   +1 more source

Mass Spectrometry‐Based Extracellular Vesicle Proteomics for Biomarker Discovery

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Extracellular vesicle (EV) proteomics has emerged as a powerful platform for decoding intercellular communication and advancing biomarker discovery across human diseases. EVs carry proteins that reflect their cells of origin, offering a minimally invasive window into physiological and pathological processes.
Ya‐Juan Liu, Juan Peng, Chao Kang
wiley   +1 more source

Strigolactone signaling: Licensing antiviral RNA interference in rice

New Plant Protection, EarlyView.
Rice grassy stunt virus (RGSV) suppresses strigolactone‐mediated antiviral RNAi through P3‐D14 interaction. Structure‐guided D14D102N editing disrupts P3 binding while maintaining SL signaling, sustaining antiviral defense and providing strong resistance without compromising plant growth or yield.
Kaibo Gu, Yanjun Li
wiley   +1 more source

Gene turnover in the common ancestor of all C4 grasses

PLANTS, PEOPLE, PLANET, EarlyView.
Understanding how plants evolve more efficient photosynthesis is important in a warming world where improving crop productivity and resilience is a global priority. By generating the first reference genomes for an early‐diverging group of grasses called the Aristidoideae, we were able to reconstruct the genetic makeup of the last common ancestor of all
Lara Pereira, Ahmed S. Alenazi, Sahr Mian, Ilia J. Leitch, Pascal‐Antoine Christin, Colin P. Osborne, Luke T. Dunning   +6 more
wiley   +1 more source

Universal and Lineage‐Specific Patterns in the Distribution of ECOD Domain Homology Groups Across Superkingdoms

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Proteins are built from modular domains that serve as fundamental units of structure and evolution. While individual domains have been extensively cataloged, their collective distribution across the lineages of life has remained poorly resolved.
Rui Guo, Jimin Pei, Jing Zhang, Qian Cong, Nick V. Grishin, R. Dustin Schaeffer   +5 more
wiley   +1 more source

Expression of human ARGONAUTE 2 inhibits endogenous microRNA activity in Arabidopsis

Frontiers in Plant Science, 2013
Plant and animal microRNA (miRNA) pathways share many analogous components, the ARGONAUTE (AGO) proteins being foremost among them. We sought to ascertain the degree of functional conservation shared by Homo sapiens ARGONAUTE 2 (HsAGO2) and Arabidopsis ...
Ira eDeveson, Junyan eLi, Anthony eMillar   +2 more
doaj   +1 more source

RNA degradomics and proteomics reveal the mechanism of dsProsβ1‐mediated proteasome targeting in Psylliodes chrysocephala

Pest Management Science, EarlyView.
RNA degradomics revealed dsProsβ1‐derived siRNA‐mediated mRNA cleavage events, mainly at uracil‐guanine and adenine‐adenine pairs. Proteasome inhibition via dsProsβ1 increased mitochondrial and cytoskeletal proteins while reducing translation‐related and mRNA‐binding proteins.
Doga Cedden, Gözde Güney, Michael Rostás, Stefan Scholten   +3 more
wiley   +1 more source

Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activity

eLife
RNA interference (RNAi) is a conserved pathway that utilizes Argonaute proteins and their associated small RNAs to exert gene regulatory function on complementary transcripts.
Shihui Chen, Carolyn Marie Phillips
doaj   +1 more source

Transcriptomic resources and functional validation of RNAi in the cabbage seedpod weevil, Ceutorhynchus obstrictus

Pest Management Science, EarlyView.
Transcriptome sequencing of Ceutorhynchus obstrictus revealed RNA interference (RNAi) targets; double‐stranded RNA microinjection reduced transcript levels and increased mortality, with αCOP and Rop showing strongest lethal effects, indicating promising RNAi‐based pest control candidates.
Apurva Vikas Sabnis, Triin Kallavus, Éricmar Avila Dos Santos, Liina Soonvald, Riina Kaasik, Silva Vilumets, Clauvis Nji Tizi Taning, Kristof De Schutter, Eve Veromann   +8 more
wiley   +1 more source

Prokaryotic Argonautes – variations on the RNA interference theme

Microbial Cell, 2014
The discovery of RNA interference (RNAi) has been a major scientific breakthrough. This RNA-guided RNA interference system plays a crucial role in a wide range of regulatory and defense mechanisms in eukaryotes.
John van der Oost, Daan C. Swarts, Matthijs M. Jore   +2 more
doaj   +1 more source