Results 31 to 40 of about 22,965 (136)

Anti-miRs competitively inhibit microRNAs in Argonaute complexes. [PDF]

PLoS ONE, 2014
MicroRNAs (miRNAs), small RNA molecules that post-transcriptionally regulate mRNA expression, are crucial in diverse developmental and physiological programs and their misregulation can lead to disease.
Daniel J Hogan, Thomas M Vincent, Sarah Fish, Eric G Marcusson, Balkrishen Bhat, B Nelson Chau, Dimitrios G Zisoulis   +6 more
doaj   +1 more source

Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

Biology Direct, 2013
tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins.
Loss-Morais Guilherme, Waterhouse Peter M, Margis Rogerio   +2 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Asgard archaea defense systems and their roles in the origin of eukaryotic immunity

Nature Communications
Dozens of new antiviral systems have been recently characterized in bacteria. Some of these systems are present in eukaryotes and appear to have originated in prokaryotes, but little is known about these defense mechanisms in archaea.
Pedro Leão, Mary E. Little, Kathryn E. Appler, Daphne Sahaya, Emily Aguilar-Pine, Kathryn Currie, Ilya J. Finkelstein, Valerie De Anda, Brett J. Baker   +8 more
doaj   +1 more source

The evolution of core proteins involved in microRNA biogenesis

BMC Evolutionary Biology, 2008
Background MicroRNAs (miRNAs) are a recently discovered class of non-coding RNAs (ncRNAs) which play important roles in eukaryotic gene regulation. miRNA biogenesis and activation is a complex process involving multiple protein catalysts and involves the
Brown James R, Dancis Barry, Murphy Dennis   +2 more
doaj   +1 more source

Modulation of miR‐23b Wnt/β‐catenin Axis Strengthens Endothelial Barrier Properties

Advanced Science, EarlyView.
Early blood‐brain barrier (BBB) disruption contributes to stroke and CNS disease pathology. miR‐23b was identified as a regulator of BBB integrity in brain endothelial cells. Inhibition of miR‐23b enhanced barrier‐associated properties, promoted repair‐related signaling, and reduced BBB leakage in experimental stroke models, supporting further ...
Victor Anthony Martinez, Rose E. Presby, Sara Nakanishi, Pallavi Gaur, Ugur Akcan, Johannes Daugaard Krog, Iben Daugaard Krog, Lipi Das, David S. Johnson, Danny Jamoul, Douglas Jury, Brian Lawson, Jacob Körbelin, Lewis M. Brown, Vilas Menon, Dritan Agalliu, Irene Munk Pedersen   +16 more
wiley   +1 more source

VCP Machinery Mediates Autophagic Degradation of Empty Argonaute

Cell Reports, 2019
Summary: The Argonaute subfamily of proteins (AGO) loads microRNAs (miRNAs) to form the effector complex that mediates target gene silencing. Empty AGO, but not miRNA-loaded AGO, is selectively degraded across species.
Hotaka Kobayashi, Keisuke Shoji, Kaori Kiyokawa, Lumi Negishi, Yukihide Tomari   +4 more
doaj   +1 more source

Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. [PDF]

PLoS ONE, 2018
Prokaryotic Argonaute proteins (pAgos) have been proposed as an alternative to the CRISPR/Cas9 platform for gene editing. Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the ...
Henriette O'Geen, Chonghua Ren, Nicole B Coggins, Sofie L Bates, David J Segal   +4 more
doaj   +1 more source

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source

Temporal Control of the TGF-β Signaling Network by Mouse ESC MicroRNA Targets of Different Affinities

Cell Reports, 2019
Summary: Although microRNAs (miRNAs) function in the control of embryonic stem cell (ESC) pluripotency, a systems-level understanding is still being developed.
Timothy J. Kelly, Anneke Brümmer, Nima Hooshdaran, Mito Tariveranmoshabad, Jesse R. Zamudio   +4 more
doaj   +1 more source