Results 91 to 100 of about 1,576,930 (245)

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Click Conjugates of Artificial Ribonucleases: Sequence Specific Cleavage with Multiple Turnover

Chemistry – A European Journal
AbstractThe azido modified RNA cleaving catalyst 2 has been attached to oligonucleotides containing alkyne linkers in a central position. The resulting conjugates hybridize specifically with complementary RNA strands and cleave them with multiple substrate turnover. RNA half‐lives are in the range of 6–7 hours (pH 8, 37 °C).
Sandra Weber, Timo Weinrich, Ute Scheffer, Elisabeth Kalden, Michael W. Göbel   +4 more
openaire   +2 more sources

Clock Genes Regulate Ca2+ Signaling and Mitochondrial Bioenergetics to Inhibit Sjögren Disease

Arthritis &Rheumatology, EarlyView.
Objective Although Ca2+ signaling and metabolism have been identified as key determinants for the development of Sjögren disease (SjD), the intricate connection between them and salivary gland physiology remains poorly understood. Methods Fluorescence‐based Ca2+ imaging, RNA sequencing, and mitochondrial activity were used to investigate the effects of
Viktor R. Drel, Manigandan Venkatesan, Rahul S. Jasrotia, Yuyang Sun, Viviane Nascimento da Conceicao, Neelanjan Vishnu, Shankara N. Varadarajan, Venugopal R. Bovilla, Rajender K. Motiani, Shobhan Gaddameedhi, Bibhuti B. Mishra, Muniswamy Madesh, Brij B. Singh   +12 more
wiley   +1 more source

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

Multivalent RNA‐Cleaving Agents on a Cubic Octameric Silsesquioxane Core

Chemistry – A European Journal, EarlyView.
RNA‐cleaving agents on a cubic octameric silsesquioxane core were synthesized and characterized. Several catalytic systems of these structures were screened using hexaribonucleotide models. The best systems were then further used to study their catalytic efficiency in cleaving a HER2 mRNA model sequence. The induced cleavage sites were also determined.
Hanni Haapsaari, Iris Tuomi, Pasi Virta
wiley   +1 more source

Site‐Specific Enzymatic Syntheses of DNA Bearing Several Modifications From RNA or DNA Templates

Chemistry – A European Journal, EarlyView.
A single‐tube protocol for enzymatic synthesis of DNA containing 1–3 site‐specific nucleobase modifications was developed based on repeated single nucleotide extension, primer extension, and exchange of template. ABSTRACT Two approaches for the enzymatic synthesis of DNA oligonucleotides (ONs) bearing multiple site‐specific modifications were explored.
Lukáš Kaiser, Veronika Raindlová, Michal Hocek   +2 more
wiley   +1 more source

Transcriptome and proteome analyses reveal divergent adaptive strategies in nasopharyngeal epithelial cells and nasopharyngeal carcinoma cells under ultra‐low background radiation

Deep Underground Science and Engineering, EarlyView.
This study demonstrates that the ultra‐low background radiation environment in deep underground laboratories significantly inhibits the proliferation of both normal nasopharyngeal epithelial cells (HNEPC) and nasopharyngeal carcinoma cells (HNE‐1) cells, with malignant cells exhibiting higher sensitivity.
Yuhao Zou, Qi Wu, Jirui Wen, Ling Wang, Can Li, Juan Cheng, Tengfei Ma, Chuhan Lv, Shixi Liu, Mingzhong Gao, Ruihua Dong, Jianxiong Zhang, Jingxuan Wu, Heping Xie, Jiang Wu, Jifeng Liu   +15 more
wiley   +1 more source

Differential sensitivity to SHH signaling and neural crest‐mediated Gas1 expression regulate jaw size during development and evolution

Developmental Dynamics, EarlyView.
Abstract Background Developmental control of jaw size is crucial to prevent birth defects and facilitate evolutionary adaptation. We have shown that jaw size is established by neural crest mesenchyme (NCM), which are progenitor cells that migrate into the mandibular primordia and produce the jaws.
Zuzana Vavrušová, Daniel B. Chu, An Nguyen, Yeniko Ta, Jennifer L. Fish, Richard A. Schneider   +5 more
wiley   +1 more source

Development of a new class of chemical and biological ultrasensors: Ribonuclease contamination and control [PDF]

, 1984
In order to define ribonuclease contamination, an assay for ribonuclease having picogram level sensitivity was established. In this assay, polycytidylic acid is digested by ribonuclease leading to smaller fragments of poly C that remain soluble after ...

core  

Urinary ribonuclease excretion in pancreatic disease.

, 1983
Urinary ribonuclease output and indices of renal tubular integrity were evaluated in control subjects and patients with pancreatic cancer, chronic pancreatitis and extrapancreatic diseases.
FABRIS C., VIANELLO, DANIELA, PICCOLI, ANTONIO, DEL FAVERO G., MEANI A., NACCARATO R., BONVICINI P., FARINI R., BROSOLO P.   +8 more
core   +1 more source