Results 71 to 80 of about 7,439 (222)

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Dual‐Responsive Hydrogels Engineer Anisotropic Cellular Microenvironment to Modulate Stem Cell Organization and Fate

Small, EarlyView.
Collagen peptides hydrogels with tunable mechanical dynamics were developed by employing physical or chemical nanoparticle crosslinkers. Dual‐responsive hydrogels with magnetic‐driven alignment were fabricated under a low magnetic field and rapid UV exposure. The presence of biophysical anisotropy, enhanced biomechanical dynamics, and growth factors—as
Hongjuan Weng, Wen Chen, Lei He, Timo Rademakers, Sabine van Rijt, Monize C. Decarli, Katrien V. Bernaerts, Lorenzo Moroni   +7 more
wiley   +1 more source

Silencing of juvenile hormone‐related genes through RNA interference leads to molt failure and high mortality in the spongy moth

Insect Science, EarlyView.
The feasibility of using RNA interference to control the globally important quarantine pest, the spongy moth. Targeting genes related to JHs play an important role in the growth and development of insects. First, the open reading frames (ORFs) of Ldjhamt and Ldjheh were identified and characterized, and the target genes were cloned and double‐stranded ...
Wenzhuai Ji, Qing Xie, Xiaoxiao Chang, Juan Shi   +3 more
wiley   +1 more source

Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate and the Poly(A) Tail of mRNA [PDF]

Messenger RNA (mRNA) drugs have attracted considerable attention as promising tools with many therapeutic applications. The efficient delivery of mRNA drugs using non-viral materials is currently being explored.
Matsuura, Kazunori, 岩崎, 崇, 4673, 00778011, 60283389, 26247, 26246, イナバ, ヒロシ, Inaba, Hiroshi, Sato, Yuki, 26248, マツウラ, カズノリ, イワサキ, タカシ, 100000881, Iwasaki, Takashi, 稲葉, 央, 100001643, 100000598, ナカムラ, ヨウコ, 4763, 30585584, 4648, 松浦, 和則, Nakamura, Yoko   +23 more
core   +1 more source

Whole‐Genome Sequencing and Resequencing of Hucho bleekeri Provides Insights into Genetic Mechanisms of Environmental Adaptation

Integrative Zoology, EarlyView.
A high‐quality chromosome‐level reference genome was constructed for Hucho bleekeri. Population structure and environmental adaptation of Hucho species were revealed by whole‐genome resequencing. ABSTRACT Salmonidae represents an important family in the study of genome evolution following genome duplication.
Yeyu Chen, Huanchao Yang, Zhao Liu, Xiaoyun Wu, Qinyao Wei, Yanling Chen, Jue Lin, Yi Yu, Quanyu Tu, Hua Li   +9 more
wiley   +1 more source

Toll‐like receptor 4 mediates lipopolysaccharide‐induced emesis

British Journal of Pharmacology, EarlyView.
Background and Purpose Lipopolysaccharide (LPS) induces inflammation and sepsis through Toll‐like receptor 4 (TLR4) activation. Common laboratory animals do not exhibit emesis, but administration of LPS in piglets, cats, and dogs induces emesis via peripheral mechanisms.
Luping Liu, Zengbing Lu, Julia Yuen Hang Liu, Man Piu Ngan, Kai‐Hei Tse, John Anthony Rudd   +5 more
wiley   +1 more source

Protein Synthesis in the Hair Follicle. II. Polysomes and Amino Acid Incorporation**From the Department of Biochemistry, University of Adelaide, Adelaide, S.A., 5000, Australia.

, 1970
Single ribosomes and polysomes were isolated from guinea pig hair follicle tissue by sucrose density gradient centrifugation and characterized by electron microscopy.
Rogers, George E., Clarke, Rodney M.
core   +1 more source

Regulation of PHOX2B gene expression by the long non‐coding natural antisense RNA PHOX2B‐AS1

The FEBS Journal, EarlyView.
PHOX2B is a transcription factor essential for autonomic nervous system development. We identify and characterize PHOX2B‐AS1, a human long non‐coding antisense transcript at the PHOX2B locus, along with its murine counterpart. Our findings reveal bidirectional transcription and reciprocal regulation: PHOX2B activates PHOX2B‐AS1, whereas PHOX2B‐AS1 ...
Simona Di Lascio, Ana Lucia Cuadros Gamboa, Martina Bertocchi, Filippo Chiesa, Francesca Cargnin, Ettore Mosca, Paride Pelucchi, Viviana Tritto, Stefania Corti, Isabella Ceccherini, Paola Riva, Roberta Benfante, Diego Fornasari   +12 more
wiley   +1 more source

Optical modulation of high-affinity biomolecules function via photochromic dyes : a step towards an artificial control of biological activity [PDF]

, 2008
Prior to this study, there has yet to be a clear demonstration of an artificial control of antibody affinity via photochromic dyes. The research described in this thesis sets out to address this by investigating photochromic dyes and their subsequent ...
Annan, Bernard Derek
core  

Click Conjugates of Artificial Ribonucleases: Sequence Specific Cleavage with Multiple Turnover

Chemistry – A European Journal
AbstractThe azido modified RNA cleaving catalyst 2 has been attached to oligonucleotides containing alkyne linkers in a central position. The resulting conjugates hybridize specifically with complementary RNA strands and cleave them with multiple substrate turnover. RNA half‐lives are in the range of 6–7 hours (pH 8, 37 °C).
Sandra Weber, Timo Weinrich, Ute Scheffer, Elisabeth Kalden, Michael W. Göbel   +4 more
openaire   +2 more sources