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Microplate quantification of enzymes of the plant ascorbate–glutathione cycle

Analytical Biochemistry, 2008
Here, we describe microplate assays for determining the specific activities of four enzymes that constitute the ascorbate-glutathione cycle: APX, MDHAR, DHAR, and GR. In plants, these enzymes play a major role in detoxifying reactive oxygen species produced in cells under environmental stress.
Ramzi, Murshed   +2 more
openaire   +2 more sources

Mechanism of selenite tolerance during barley germination: A combination of tissue selenium metabolism alterations and ascorbate-glutathione cycle modulation.

Plant physiology and biochemistry : PPB, 2023
Selenite is widely used to increase Selenium (Se) content in cereals, however excessive selenite may be toxic to plant growth. In this study, barley was malted to elucidate the action mechanism of selenite in the generation and detoxification of ...
Chao Cheng   +4 more
semanticscholar   +1 more source

Ascorbate-Glutathione Cycle and Abiotic Stress Tolerance in Plants

2017
Plants confront fluctuating and in some cases intense environmental conditions, such as changes in irradiation, water availability, extreme temperatures, mineral nutrient accessibility, and air pollutants exposition among others. In order to face abiotic stress situations, the redox buffer capacity, mainly represented by ascorbic acid (AsA) and ...
Bartoli, Carlos Guillermo   +4 more
openaire   +2 more sources

Specific features of the ascorbate/glutathione cycle in cultured protoplasts

Plant Cell Reports, 1999
Protoplasts isolated from Nicotiana tabacum (L.) leaves were cultured for 6 days in liquid medium and some features of their antioxidant capacity were investigated. Ascorbate exported into the culture medium was oxidized non-enzymically, whereas important modifications of the enzymic scavenging activity were detected inside protoplasts.
A. de Marco, K. A. Roubelakis-Angelakis
openaire   +1 more source

Diurnal changes of the ascorbate-glutathione cycle components in wheat genotypes exposed to drought

Functional Plant Biology, 2020
The ascorbate-glutathione (AsA-GSH) cycle is a major pathway of H2O2 scavenging in plants. The effect of diurnal variations in hydrogen peroxide (H2O2) content, the intensity of lipid peroxidation (malondialdehyde, MDA), photosynthesis, antioxidants and antioxidative enzyme activities involved in AsA-GSH metabolism has been studied comparatively in ...
Durna R. Aliyeva   +3 more
openaire   +2 more sources

Responses of the Xanthophyll Cycle Pool and Ascorbate–Glutathione Cycle to Ozone Stress in Two Tobacco Cultivars

Free Radical Research, 1999
Plants of Nicotiana tabacum (O3-tolerant cv Bel-B and O3-sensitive cv Bel-W3) were exposed to 150 ppb of ozone for 5 h; the fumigation produced visual injury in mature leaves, particularly in Bel-W3. After O3-treatment the pigments of the xanthophyll cycle pool decreased in both cvs, with a strong reduction in violaxanthin content, while antheraxanthin
S, Pasqualini   +3 more
openaire   +2 more sources

The role of nitrate reductase in brassinosteroid-induced endogenous nitric oxide generation to improve cadmium stress tolerance of pepper plants by upregulating the ascorbate-glutathione cycle.

Ecotoxicology and Environmental Safety, 2020
A study was performed to assess if nitrate reductase (NR) participated in brassinosteroid (BR)-induced cadmium (Cd) stress tolerance primarily by accelerating the ascorbate-glutathione (AsA-GSH) cycle.
C. Kaya   +3 more
semanticscholar   +1 more source

Regulation of the ascorbate-glutathione cycle in wild almond during drought stress

Russian Journal of Plant Physiology, 2011
In wild species of almond (Prunus spp.), the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR), as well as the levels of ascorbate/glutathione pools and H2O2 were subjected to water deficit and shade conditions. After 60 days of water shortage, the species
Sorkheh K   +4 more
openaire   +2 more sources

Regulation of ascorbate-glutathione cycle by exogenous nitric oxide and hydrogen peroxide in soybean roots under arsenate stress.

Journal of Hazardous Materials, 2020
The role of nitric oxide (NO) and hydrogen peroxide (H2O2) is well known for regulating plant abiotic stress responses. However, underlying mechanisms are still poorly understood.
Samiksha Singh   +15 more
semanticscholar   +1 more source

Delayed senescence of kiwifruit by p-coumaric acid pretreatment during storage at 20 °C: Toward regulating the ascorbate–glutathione cycle and phenolic anabolism

, 2021
‘Xuxiang’ kiwifruit were pretreated with 0.5 mmol L−1 p-coumaric acid (p-CA) and then stored at 20 °C for up to 20 d to investigate the influence of p-CA and possible mechanisms on senescence.
Maomao Cao   +5 more
semanticscholar   +1 more source

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