Results 121 to 130 of about 11,516 (233)

NIBV Induces Incomplete Autophagy via AMPK‐TFEB, Causing Kidney Injury in Chicks

Advanced Science, Volume 13, Issue 34, 19 June 2026.
This study reveals that NIBV can inhibit the nuclear translocation of TFEB by suppressing the expression of AMPK, leading to the blockade of autophagolysosomal functions, in turn increasing NIBV replication and triggering severe kidney injury in chicks.
Cheng Huang, Yizhou Zeng, Yunfeng Chen, Zhengqing Li, Salma Mbarouk Omar, Shengwei Zhong, Ping Liu, Zhanhong Zheng, Gaofeng Cai, Xiaona Gao, Xiaoquan Guo   +10 more
wiley   +1 more source

MGF300 genes are critical to ASFV replication in primary porcine alveolar macrophages (PAMs).

, 2023
The (A) Schematic diagram depicting the deletion of multigene family genes (MGFs) in the left variable region (LVR) of the ASFV HLJ/18 strain (ASFV-WT) genome.
Shujian Huang (1899610), Yuan Sun (15554), Zhanhao Lu (16819609), Rui Luo (169614), Li Pan (49390), Tao Wang (12008), Jing Zhang (23775), Lian-Feng Li (14680123), Yong-Hui Zheng (50334), Huanjie Zhai (11886013), Hua-Ji Qiu (3967025), Jing Lan (1699462)   +11 more
core   +1 more source

Full-Length ASFV B646L Gene Sequencing by Nanopore Offers a Simple and Rapid Approach for Identifying ASFV Genotypes

Viruses
African swine fever (ASF) is an acute, highly hemorrhagic viral disease in domestic pigs and wild boars. The disease is caused by African swine fever virus, a double stranded DNA virus of the Asfarviridae family. ASF can be classified into 25 different genotypes, based on a 478 bp fragment corresponding to the C-terminal sequence of the B646L gene ...
Vivian O’Donnell, Edward Spinard, Lizhe Xu, Amy Berninger, Roger W. Barrette, Douglas P. Gladue, Bonto Faburay   +6 more
openaire   +3 more sources

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, Volume 13, Issue 32, 9 June 2026.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

IFN-α treatment does not affect the mRNA level of p72 of ASFV and ASFV-intB318L.

PAMs were infected with ASFV-WT or ASFV-intB318L (MOI = 1) for 24 h, and then treated with IFN-α (1 μg/mL) for another 12 h. The mRNA levels of ASFV p72 were analyzed by qPCR. Data represent three independent experiments with three biological replicates (
Chunying Feng (12428466), Weiye Chen (3234612), Jiangnan Li (658317), Qiongqiong Zhou (92521), Zhaoxia Zhang (3714328), Xiaohong Liu (50816), Liang Hu (202392), Yuntao Guan (368332), Changjiang Weng (8127537), Guangqiang Ye (18369131), Xijun He (663738), Zhengshuang Wu (18369134), Xianfeng Zhang (295346), Dongming Zhao (296447), Li Huang (107408), Hongyang Liu (303058), Zhigao Bu (146375), Hefeng Chen (7834751)   +17 more
core   +1 more source

Immunogenicity assessment and epitope mapping of the ASFV proteome by profiling serum antibodies with ASFV antigen phage libraries

Communications Biology
The continued spread and regular outbreaks of African swine fever (ASF) have severely threatened the pig-related industries, causing economic losses. African swine fever virus (ASFV) encoded more than 150 different proteins, but the biological characteristics of the majority of these proteins remain unknown.
Lerong Ma, Zhen Weng, Yuanzhu Zhang, Zhi Cao, Xingjun Ke, Ruize Sun, Yin Xie, Mengjie Lian, Lin Yang, Hongming Yuan, Zicong Xie, HongSheng Ouyang, Daxin Pang, Dongmei Lv   +13 more
openaire   +2 more sources

Nanobodies: A Promising Toolkit for Diagnostic Applications

SmartMat, Volume 7, Issue 3, June 2026.
This review focuses on camelid‐derived nanobodies (VHHs) and explains how their small size and high stability support robust diagnostic design. Applications across ELISA, lateral flow assays, and PET/SPECT imaging are summarized, along with clinical progress such as caplacizumab.
Wei Wu, Abid Ali, Li Sun, Jiahao Hu, Lina Hameed, Saba Shafi, Ali Raza, Khuzin Dinislam, Wenjing Xing, Long Rong, Jie Lin, Jian Ma   +11 more
wiley   +1 more source

Epidemiological analysis of African swine fever in the European Union during 2025

EFSA Journal, Volume 24, Issue 5, May 2026.
Abstract During 2025, the number of EU Member States affected by African swine fever (ASF) increased from 13 to 14, following the detection of African swine fever virus (ASFV) in wild boar in Spain. The number of ASF outbreaks in domestic pigs in the EU increased from 333 in 2024 to 585 in 2025, largely driven by Romania, accounting for 81% of ...
European Food Safety Authority (EFSA), Karl Ståhl, Anette Boklund, Tomasz Podgórski, Timothée Vergne, Lina Mur, Roxani Aminalragia‐Giamini, Alessia Rusinà, Eliana Lima   +8 more
wiley   +1 more source

The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine

Viruses, 2020
African swine fever virus (ASFV) is the causative agent of the African swine fever (ASF) epizootic currently affecting pigs throughout Eurasia, causing significant economic losses in the swine industry.
Elizabeth. Ramirez-Medina, Elizabeth. A. Vuono, Ayushi. Rai, Sarah. Pruitt, Ediane. Silva, Lauro. Velazquez-Salinas, James. Zhu, Manuel. V. Borca, Douglas. P. Gladue   +8 more
doaj   +1 more source

Droplet Digital CRISPR for Nucleic Acid Detection

Advanced Science, Volume 13, Issue 14, 9 March 2026.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber, Ming Li   +4 more
wiley   +1 more source