Results 101 to 110 of about 7,847 (206)

Immunogenicity assessment and epitope mapping of the ASFV proteome by profiling serum antibodies with ASFV antigen phage libraries

open access: yesCommunications Biology
The continued spread and regular outbreaks of African swine fever (ASF) have severely threatened the pig-related industries, causing economic losses. African swine fever virus (ASFV) encoded more than 150 different proteins, but the biological characteristics of the majority of these proteins remain unknown.
Lerong Ma   +13 more
openaire   +2 more sources

Full-Length ASFV B646L Gene Sequencing by Nanopore Offers a Simple and Rapid Approach for Identifying ASFV Genotypes

open access: yesViruses
African swine fever (ASF) is an acute, highly hemorrhagic viral disease in domestic pigs and wild boars. The disease is caused by African swine fever virus, a double stranded DNA virus of the Asfarviridae family. ASF can be classified into 25 different genotypes, based on a 478 bp fragment corresponding to the C-terminal sequence of the B646L gene ...
Vivian O’Donnell   +6 more
openaire   +3 more sources

Droplet Digital CRISPR for Nucleic Acid Detection

open access: yesAdvanced Science, Volume 13, Issue 14, 9 March 2026.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang   +4 more
wiley   +1 more source

Digital Point‐of‐Care Testing Device Based on Bioluminescent Pyrophosphate in Real‐Time and Recombinase‐aid Amplification

open access: yesAdvanced Intelligent Systems, Volume 8, Issue 3, March 2026.
A point‐of‐care testing method based on solvent‐responsive magnetic beads suitable for large‐area diagnosis and mutation screening in remote areas was developed to detect it by combining bioluminescenct pyrophosphate in real‐time technology and recombinase‐aid amplification technology.
Zhongjie Fei   +8 more
wiley   +1 more source

IFN-α treatment does not affect the mRNA level of p72 of ASFV and ASFV-intB318L.

open access: yes
PAMs were infected with ASFV-WT or ASFV-intB318L (MOI = 1) for 24 h, and then treated with IFN-α (1 μg/mL) for another 12 h. The mRNA levels of ASFV p72 were analyzed by qPCR. Data represent three independent experiments with three biological replicates (
Chunying Feng (12428466)   +17 more
core   +1 more source

Safeguarding a Flagship Species: Integrated Surveillance of Cross‐Species Pathogen Transmission in Giant Panda Ecosystems

open access: yesEcology and Evolution, Volume 16, Issue 3, March 2026.
Emerging infectious diseases increasingly threaten giant pandas and cohabiting species. This review analyzes pathogen transmission risks from domestic animals, wildlife, and vectors and proposes an integrated genomic surveillance framework for early warning and biodiversity conservation.
Xiaoli Sun   +10 more
wiley   +1 more source

Seroprevalence of African Swine Fever Virus and Farm Management Practices in Nepal: A Cross‐Sectional Study

open access: yesVeterinary Medicine and Science, Volume 12, Issue 2, March 2026.
A cross‐sectional study on seroprevalence of African swine fever virus was conducted across pig farms in Bagmati Province. Of the 324 samples screened, seven tested positive, with an overall seroprevalence of 2.16% . ABSTRACT Background African swine fever (ASF) is a contagious fatal viral disease of pigs caused by ASF virus (ASFV).
Meera Prajapati   +5 more
wiley   +1 more source

Advances in Double‐Stranded DNA Targeting Technologies

open access: yesExploration, Volume 6, Issue 1, February 2026.
Double‐stranded DNA (dsDNA) plays a crucial role in genetic information storage and disease management, but its inherent stability limits access to internal bases. To overcome this challenge, various high‐specificity molecular targeting technologies have been developed to destabilize the DNA structure.
Zuhao Shen   +8 more
wiley   +1 more source

Whole genome sequencing of ASFV-intB318L.

open access: yes
African swine fever (ASF) is an acute, hemorrhagic, and severe infectious disease caused by the ASF virus (ASFV). ASFV has evolved multiple strategies to escape host antiviral immune responses.
Chunying Feng (12428466)   +17 more
core   +1 more source

ASFV outbreak locations and cases in different time windows.

open access: yes, 2022
(a) A frequency plot of ASFV records in 33 months, (b) a frequency plot with respect to months (Jan to Dec), (c) a frequency plot with respect to year since the first confirmed case, and (d) the daily growth of ASFV cases in wild boar since October 3rd ...
Gab-Sue Jang (14070449)   +4 more
core   +1 more source

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