Results 61 to 70 of about 9,741 (217)

Machine‐Learning Microfluidic Minute‐Scale Microorganism Metrics Monitoring(M6)

Advanced Science, EarlyView.
ABSTRACT On‐site monitoring of microorganisms remains challenging because of low concentrations, strong background interference, and dynamic aerosol diffusion, particularly for aerosol‐transmitted pathogens. Here, we report a rapid detection platform that integrates a Puri‐focusing microfluidic chip, electrochemical impedance spectroscopy (EIS), and ...
Ning Yang, Jiahao Ding, Si Chen, Lijie Yan, Shichao Ding, Lavonda Li, Junyi Sun, Haodong Liu, Tongge Li, Ning Liu, Mingji Wei, Xiaoyong Zhu, Xiaobo Zou, Shouqi Yuan, Xingcai Zhang   +14 more
wiley   +1 more source

Simulation of Spread of African Swine Fever, Including the Effects of Residues from Dead Animals [PDF]

, 2016
To study the spread of African swine fever (ASF) within a pig unit and the impact of unit size on ASF spread, a simulation model was created. In the model, an animal can be in one of the following stages: susceptible, latent, subclinical, clinical or ...
Boklund, Anette, Bøtner, Anette, Hisham Beshara Halasa, Tariq, Thulke, Hans-Hermann, Toft, Nils   +4 more
core   +5 more sources

Pathology of porcine peripheral white blood cells during infection with African swine fever virus [PDF]

, 2012
Background African swine fever virus (ASFV) is the causative agent of African swine fever (ASF) that is the significant disease of domestic pigs. Several studies showed that ASFV can influence on porcine blood cells in vitro.
Zaven Karalyan, Hovakim Zakaryan, Hranush Arzumanyan, Khachik Sargsyan, Henrik Voskanyan, Lina Hakobyan, Liana Abroyan, Aida Avetisyan, Elena Karalova   +8 more
core   +2 more sources

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, EarlyView.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

Investigating the Heat Stability and Inactivation Conditions of Decapod Iridescent Virus 1 (DIV1)

Animal Research and One Health, EarlyView.
Heat treatment effectively inactivates decapod iridescent virus 1 (DIV1) under milder conditions than current WOAH recommendations. DIV1 infectivity was eliminated at 56°C for 30 min, 60°C for 15 min, or 70°C for 1 min, providing optimized heat treatment strategies for aquaculture biosecurity and seafood safety.
Yonghui Feng, Xiaomeng Guo, Jingyi Xing, Liang Qiu, Jie Huang   +4 more
wiley   +1 more source

Function investigation of p11.5 in ASFV infection

Virologica Sinica
Virus replication relies on complex interactions between viral proteins. In the case of African swine fever virus (ASFV), only a few such interactions have been identified so far. In this study, we demonstrate that ASFV protein p72 interacts with p11.5 using co-immunoprecipitation and liquid chromatography-mass spectrometry (LC-MS).
Dan Yin, Bin Shi, Renhao Geng, Yingnan Liu, Lang Gong, Hongxia Shao, Kun Qian, Hongjun Chen, Aijian Qin   +8 more
openaire   +2 more sources

Development and In Vivo Evaluation of a MGF110-1L Deletion Mutant in African Swine Fever Strain Georgia

Viruses, 2021
African swine fever (ASF) is currently causing an epizootic, affecting pigs throughout Eurasia, and causing significant economic losses in the swine industry.
Elizabeth Ramirez-Medina, Elizabeth Vuono, Sarah Pruitt, Ayushi Rai, Ediane Silva, Nallely Espinoza, James Zhu, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue   +9 more
doaj   +1 more source

First detection of African Swine Fever Virus in Ornithodoros porcinus in Madagascar and new insights into tick distribution and taxonomy [PDF]

, 2010
Background African Swine Fever Virus has devastated more than the half of the domestic pig population in Madagascar since its introduction, probably in 1997-1998.
Julie Ravaomanana, Vincent Michaud, Ferran Jori, Abel Andriatsimahavandy, François Roger, Emmanuel Albina, Laurence Vial   +6 more
core   +4 more sources

Droplet Digital CRISPR for Nucleic Acid Detection

Advanced Science, Volume 13, Issue 14, 9 March 2026.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber, Ming Li   +4 more
wiley   +1 more source

Deletion of an African Swine Fever Virus ATP-Dependent RNA Helicase QP509L from the Highly Virulent Georgia 2010 Strain Does Not Affect Replication or Virulence

Viruses, 2022
African swine fever virus (ASFV) produces a lethal disease (ASF) in domestic pigs, which is currently causing a pandemic deteriorating pig production across Eurasia.
Elizabeth Ramirez-Medina, Elisabeth A. Vuono, Sarah Pruitt, Ayushi Rai, Nallely Espinoza, Edward Spinard, Alyssa Valladares, Ediane Silva, Lauro Velazquez-Salinas, Manuel V. Borca, Douglas P. Gladue   +10 more
doaj   +1 more source