Results 51 to 60 of about 541 (128)

A Strategy for Rapid Acquisition of the β-D-Fructofuranosidase Gene through Chemical Synthesis and New Function of Its Encoded Enzyme to Improve Gel Properties during Yogurt Processing

Foods, 2023
A chemical gene synthesis strategy was developed in order to obtain β-D-fructofuranosidase, and a novel gene, AlFFase3, was characterized from Aspergillus luchuensis and expressed in Escherichia coli.
Zhou Chen, Yimei Shen, Jiangqi Xu
doaj   +1 more source

Analysis of genomic characteristics and their influence on metabolism in Aspergillus luchuensis albino mutants using genome sequencing

Fungal Genetics and Biology, 2021
Black Aspergillus luchuensis and its white albino mutant are essential fungi for making alcoholic beverages in Japan. A large number of industrial strains have been created using novel isolation or gene/genome mutation techniques. Such mutations influence metabolic and phenotypic characteristics in industrial strains, but few comparative studies of ...
Nozomi Yamamoto, Naoki Watarai, Hitoshi Koyano, Kazunori Sawada, Atsushi Toyoda, Ken Kurokawa, Takuji Yamada   +6 more
openaire   +2 more sources

A CRISPR/Cas9-mediated gene knockout system inAspergillus luchuensismut.kawachii

Bioscience, Biotechnology, and Biochemistry, 2020
AbstractWe developed an approach to genome editing of the white koji fungus, Aspergillus luchuensis mut. kawachii using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. Co-transformation of AMA1-based Cas9 and gRNA expression plasmids achieved efficient gene knockout in A. kawachii.
Chihiro, Kadooka, Masaaki, Yamaguchi, Kayu, Okutsu, Yumiko, Yoshizaki, Kazunori, Takamine, Takuya, Katayama, Jun-Ichi, Maruyama, Hisanori, Tamaki, Taiki, Futagami   +8 more
openaire   +2 more sources

Isolation methods of high glycosidase-producing mutants of Aspergillus luchuensis and its mutated genes

Bioscience, Biotechnology, and Biochemistry, 2020
ABSTRACT High glycosidase-producing strains of Aspergillus luchuensis were isolated from 2-deoxyglucose (2-DG) resistant mutants. α-Amylase, exo-α-1,4-glucosidase, β-glucosidase and β-xylosidase activity in the mutants was ~3, ~2, ~4 and ~2.5 times higher than the parental strain RIB2604 on koji-making conditions, respectively.
Kazuya, Tomimoto, Yukio, Osafune, Dararat, Kakizono, Jinshun, Han, Nobuhiko, Mukai   +4 more
openaire   +2 more sources

Reduced Activity of Triazole Antifungal Agents Against Aspergillus tubingensis From a Global Collection of Genomically‐Characterized Aspergillus Section Nigri Clinical Isolates (2020–2023)

Mycoses, Volume 69, Issue 7, July 2026.
ABSTRACT Background The black moulds comprising Aspergillus section Nigri are clinically important organisms causing aspergillosis and otomycosis; triazole (azole) antifungal agents represent the primary prophylaxis and treatment option for these infections.
John H. Kimbrough, Marisa L. Winkler, Maura H. Karr, Jessica M. Ewald, Mariana Castanheira   +4 more
wiley   +1 more source

Diversity in Secondary Metabolites Including Mycotoxins from Strains of Aspergillus Section Nigri Isolated from Raw Cashew Nuts from Benin, West Africa.

PLoS ONE, 2016
In a previous study, raw cashew kernels were assayed for the fungal contamination focusing on strains belonging to the genus Aspergillus and on aflatoxins producers.
Yendouban Lamboni, Kristian F Nielsen, Anita R Linnemann, Yüksel Gezgin, Kerstin Hell, Martinus J R Nout, Eddy J Smid, Manuele Tamo, Martinus A J S van Boekel, Jakob Blæsbjerg Hoof, Jens Christian Frisvad   +10 more
doaj   +1 more source

Safety evaluation of a food enzyme containing endo‐polygalacturonase and pectin lyase activities from the non‐genetically modified Aspergillus luchuensis strain NZYM‐XA

EFSA Journal, Volume 24, Issue 7, July 2026.
Abstract The food enzyme containing endo‐polygalacturonase (1,4‐α‐d‐galacturonan glycanohydrolase; EC 3.2.1.15) and pectin lyase (1,4‐6‐O‐methyl‐α‐d‐galacturonan lyase; EC 4.2.2.10) activities is produced with the non‐genetically modified microorganism Aspergillus luchuensis strain NZYM‐XA by Novozymes A/S.
EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Henk Van Loveren, Laurence Vernis, Pier Sandro Cocconcelli, Cristina Andreoli, Andrea Terron, Andrey Yurkov, Jaime Aguilera, Magdalena Andryszkiewicz, Ana Criado, Francesco Pesce, Yi Liu   +21 more
wiley   +1 more source

Safety evaluation of the food enzyme containing endo‐polygalacturonase and pectinesterase activities from the non‐genetically modified Aspergillus luchuensis strain AP2 903‐6

EFSA Journal, Volume 24, Issue 7, July 2026.
Abstract The food enzyme containing endo‐polygalacturonase (1,4‐α‐d‐galacturonan glycanohydrolase; EC 3.2.1.15) and pectinesterase (pectin pectylhydrolase; EC 3.1.1.11) activities is produced with the non‐genetically modified Aspergillus luchuensis strain AP2 903‐6 by Shin Nihon Chemical Co. Ltd. The food enzyme was considered free from viable cells of
EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Henk Van Loveren, Laurence Vernis, Cristina Andreoli, Andrea Terron, Andrey Yurkov, Jaime Aguilera, Magdalena Andryszkiewicz, Daniele Cavanna, Ana Criado, Yi Liu   +20 more
wiley   +1 more source

Development of enzyme technology for Aspergillus oryzae, A. sojae, and A. luchuensis,the national microorganisms of Japan [PDF]

Bioscience, Biotechnology, and Biochemistry, 2016
AbstractThis paper describes the modern enzymology in Japanese bioindustries. The invention of Takadiastase by Jokiti Takamine in 1894 has revolutionized the world of industrial enzyme production by fermentation. In 1949, a new γ-amylase (glucan 1,4-α-glucosidase, EC 3.2.1.3) from A. luchuensis (formerly designated as A. awamori), was found by Kitahara.
openaire   +2 more sources

NADPH Dehydrogenase Gene Regulates Energy Distribution and Fatty Acid Metabolism During Fruiting Body Formation in the Filamentous Fungus Podospora anserina

Microbial Biotechnology, Volume 19, Issue 6, June 2026.
NADPH dehydrogenase gene deletion significantly triggered NADPH/NADP+ increase in mutant strains. NADPH gene deletion promoted Fatty acid biosynthesis and repressed β‐oxidation. NADPH gene deletion decreased oxidative phosphorylation and increased substrate‐level phosphorylation. Mutants exhibited hypersensitivity to oxidative stress, but promoted more
Yanling Qiu, Amechi S. Nwankwegu, Yue Sun, Yuanjing Li, Weizhao Chen, Gang Liu, Ning Xie   +6 more
wiley   +1 more source