Results 61 to 70 of about 1,822,086 (171)

High‐Content CRISPR Screening: Methods and Applications

MedComm, Volume 7, Issue 9, September 2026.
High‐content CRISPR screening represents a paradigm shift in functional genomics, moving beyond traditional survival‐based readouts to enable multidimensional mapping of genotype–phenotype relationships. This review systematically outlines the methodological evolution of this approach, detailing advances in perturbation modalities, delivery systems ...
Yike Zhang, Yimeng Zhang, Xudong Tang, Xinyu Deng, Yitian Zhou, Yanfeng Wu, Jianhua Luo   +6 more
wiley   +1 more source

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, Volume 20, Issue 6, Page 1556-1590, June 2026.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Polymorphisms of the ATP-binding cassette sterol efflux transporter genes g5 and g8 in cardiovascular diseases and type 2 diabetes mellitus

Ateroscleroz
Mutations with a decrease in the expression and function of the of the ATP-binding cassette genes proteins ABCG5 and ABCG8, as the main sterol efflux transporters, lead to the accumulation of xenosterols in plasma associated with changes in the lipid profile, hyperglycemia and the risk of cardiovascular diseases (CVD) and type 2 diabetes mellitus (DM2).
I. N. Grigor’eva, T. E. Notova, T. S. Suvorova, D. L. Nepomnyashchikh   +3 more
openaire   +1 more source

Therapeutic Poxviruses Induce the Secretion of Immunostimulating and Anti‐Tumoral Extracellular Vesicles

Journal of Extracellular Vesicles, Volume 15, Issue 6, June 2026.
Immune cells infected by therapeutic poxviruses release increased amounts of extracellular vesicles bearing viral proteins and therapeutic payloads. Such EVs have the ability to activate a CD8+ T‐cell mediated antitumoral immune response and decrease tumor growth.
Lucas Walther, Vincent Mittelheisser, Marie‐Christine Claudepierre, Louis Bochler, Magali Rompais, Jules Deforges, Nathalie Silvestre, Annabel Larnicol, Christine Carapito, Eric Quémeneur, Jacky G Goetz, Karola Rittner, Vincent Hyenne   +12 more
wiley   +1 more source

Endothelial Integrin‐Linked Kinase (ILK) Deficiency Promotes Endothelial Activation and Cardiovascular Dysfunction via Receptor Interacting Protein Kinase‐1 (RIPK1) Enriched‐Extracellular Vesicle Signalling

Journal of Extracellular Vesicles, Volume 15, Issue 6, June 2026.
Endothelial ILK loss drives RIPK1‐enriched EVs that propagate systemic endothelial activation and cardiovascular remodelling. EV cargo remodelling induces early endothelial injury, microvascular dysfunction and chronic cardiac alterations through a RIPK1 dependent pathway.
Alberto Cook‐Calvete, María Delgado‐Marín, Sara Jorquera Ortega, Silvia Moreta, Mercedes Castro‐Pinto, José López‐Menendez, Blanca Fernandez‐Rodriguez, Julia García‐García, Carlos Zaragoza, Marta Saura   +9 more
wiley   +1 more source

Adaptive Laboratory Evolution Unlocks Membrane Permeability as a Key Limitation in Long‐Chain Alcohol Metabolism by Pseudomonas putida KT2440

Microbial Biotechnology, Volume 19, Issue 6, June 2026.
Pseudomonas putida KT2440 grows on polyethylene‐derived long‐chain alcohols (C16OH, C20OH) as sole carbon sources. Adaptive laboratory evolution rapidly increases growth, converging on envelope‐associated mutations. Expressing the outer‐membrane transporter AlkL recapitulates much of the evolved phenotype, identifying substrate access and membrane ...
Raul Mireles, Lianet Noda‐García
wiley   +1 more source

Expressions of Kisspeptin System and Ki‐67 in the Reproductive Tissues of Cyclic Bitches

Reproduction in Domestic Animals, Volume 61, Issue 4, April 2026.
ABSTRACT Kisspeptin is a peptide that plays a pivotal role in the central regulation of gonadotropins. It is regarded as a key regulator of reproductive processes, including follicular and luteal development and endometrial remodelling. The objective of this study was to investigate the expression of KISS1, its receptor KISS1R and the proliferation ...
Özgecan Korkmaz Ağaoğlu, Ali Reha Ağaoğlu, Özlem Özmen, Gökhan Bozkurt, Şerife Taşan   +4 more
wiley   +1 more source

Systematic Identification of Molecular Signatures Dictating Therapeutic Effects of Clinically First‐Line Chemotherapy Regimens for Human Gastric Cancer Patients Based on Organoid Model

MedComm, Volume 7, Issue 3, March 2026.
Chemotherapy is the mainstay in the treatment of advanced gastric cancer (GC); yet, GC showed diverse responses to first‐line chemotherapy regimens and the underlying molecular basis is still not clear. Here, we established a system that combined organoid‐based chemotherapy regimen screening and transcriptome‐based evaluation to identify underlying ...
Jingwei Yang, Shuyue Qi, Yuan Gao, Jiansen Lu, Lin Deng, Xinglong Wu, Yifei Zhao, Yun Liu, Yanpeng Ma, Jiagui Song, Lixiang Xue, Lu Wen, Wei Fu, Fuchou Tang, Xin Zhou   +14 more
wiley   +1 more source

Structural and functional fingerprint of the mitochondrial ATP-binding cassette transporter Md11 from Saccharomyces cerevisiae

, 2006
The ATP-binding cassette half-transporter Mdl1 from Saccharomyces cerevisiae has been proposed to be involved in the quality control of misassembled respiratory chain complexes by exporting degradation products generated by the m-AAA proteases from the ...
Hofacker, Matthias, Zutz, A., Tampé, Robert, Gompf, S, Model, K., Presenti, C., Haase, W., Zutz, Ariane, K. Model, Presenti, C, R. Tampe, Model, K, Tampé, R., C. van der Does, Hofacker, M, S. Gompf, Gompf, Simone, van der Does, C., M. Hofacker, Tampé, R, Haase, W, A. Zutz, W. Haase, Haase, Winfried, Model, Kirstin, Hofacker, M., Presenti, Chiara, Zutz, A, Gompf, S., C. Presenti, Does, Chris van der   +30 more
core   +2 more sources

Emodin Improved the Hepatic Metabolic Dysfunction Induced by High Plant‐Protein Diet in Pengze Crucian Carp (Carassius auratus var. Pengze)

Aquaculture Nutrition, Volume 2026, Issue 1, 2026.
High plant‐protein (HP) diets could induce bile acid (BA) imbalance and lipid metabolism disorder in fish, and emodin supplementation has been reported to increase serum BA levels and improve lipid metabolism with a lack of understanding of its underlying mechanism.
Kaikai Shen, Yongfa Huang, Zhenwei Lu, Jianqin Li, Jingjie Zhang, Ao Luo, Baoqing Hu, Jingen Xu, Jiamin Li, Chungen Wen, Gang Yang, Chuanpeng Zhou   +11 more
wiley   +1 more source