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AbstractThe term “autolysis” was introduced into biological literature by SALKOVSKY [1]. Ever since it began to be used to designate self‐digestion of cells under the action of their own intracellular enzymes. This definition is sufficiently satisfactory with regard to bacteria, and there are published lots of original and review papers dealing ...
T. L. Babayan, M. G. Bezrukov
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Spermatozoal Hapten Gained via Autolysis
Archives of Andrology, 1980Using autolysis different spermatozoa surface peptides were solubilized. The resulting low molecular weight compounds were further separated by gel-chromatography on Biogel P-4, thin-layer chromatography on Cel 400 and thin-layer-electrophoresis. After the conjugation of the compound with cytochrome C a conformation-independent determinant was detected
L, Mettler, H, Skrabei
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Autolysis Studies of Cathepsin D
Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1982Incubation of the single polypeptide chain cathepsin D from bovine spleen at pH 3.5, resulted in the fragmentation of the molecule. This was followed by gel electrophoresis in the presence of dodecyl sulphate, gel filtration, circular dichroism and enzyme activity measurements.
T, Lah, V, Turk
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Studies in Autolysis. I. Autolysis in Seeds
Transactions of the Kansas Academy of Science (1903-), 1929Autodigestion has been a matter of research for nearly fifty years among physiological chemists. The original meaning of the term as given by Jacoby (1), who carried on the work started by Hofmeister in the latter part of the Nineteenth Century, is still accepted by workers.
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DURING the recrystallization of pepsin by the method of Northrop1, which involves heating a solution of the enzyme to 45° C. at pH 4, followed by cooling, it was noticed that the warm solution deposited a considerable quantity of needle crystals totally different from crystalline pepsin; these were identified as tyrosine.
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