Results 121 to 130 of about 58,150 (299)

Electron microscopy of autophagosomes in RV-infected cells.

, 2014
High-definition electron microscopy of non-infected (A) and RV-infected (SA11 strain; MOI, 250 VFU/ml) (B and C) MA104 cells at 14 hpi. V, viroplasms; Nu, nucleus; black arrows, AVi (early/initial autophagic vacuoles corresponding to autophagosomes ...
Miguel Mano (228671), Francesca Arnoldi (87304), Peter Wild (18726), Catherine Eichwald (126512), Oscar R. Burrone (126517), Elisabeth M. Schraner (126515), Giuditta De Lorenzo (552196)   +6 more
core   +1 more source

Targeting the HSPA8‐CMA‐ATP6V1A Axis Triggers Lysosomal Hyperacidification and Catastrophic Vacuolation in Prostate Cancer

Advanced Science, EarlyView.
Our experimental evidence supports a model in which ALO targets the HSPA8‐CMA‐ATP6V1A axis to induce lysosomal hyperacidification and initiate osmotic and lipidomic stress. These changes are associated with LMP and loss of lysosomal integrity in prostate cancer cells.
Bingzheng An, Ze Gao, Shuo Chen, Liwei Meng, Chen Zhang, Kefan Song, Haochen Cui, Lei Yan, Zhiqing Fang   +8 more
wiley   +1 more source

Lysosomal targeting of autophagosomes by the TECPR domain of TECPR2

, 2020
TECPR2 (tectonin beta-propeller repeat containing 2) is a large, multi-domain protein comprised of an amino-terminal WD domain, a middle unstructured region and a carboxy-terminal TEPCR domain comprises of six TECPR repeats followed by a functional LIR ...
Christian Behrends (673832), Bruria Ben-Zeev (2138866), Franziska Eck (9664599), Gali Heimer (9664596), Karsten Nalbach (9664602), Oren Shatz (9664605), Bat-Chen Tamim-Yecheskel (9664587), Milana Fraiberg (4344199), Kamilya Kokabi (9664590), Nemanja Subic (9664593), Zvulun Elazar (3713575)   +10 more
core   +1 more source

A) Location of initial autophagosomes (GFP–LC3-positive vesicles) and of newly appearing autophagosomes (see arrowheads for location of their appearance) during a 5-min video

, 2011
B) Distance of initial and newly appearing autophagosomes from the MTOC of three different cells after 30- to 120-min starvation in serum-free CO-independent medium (black bar indicates mean distance of autophagosomes from the MTOC, and plus sign ...
Luca Jahreiss (45857), David C Rubinsztein (23767), Fiona M Menzies (45858)   +2 more
core   +1 more source

Molecular and Cellular Hallmarks of Age‐Related Vestibular Hair Cell Degeneration

Advanced Science, EarlyView.
This study utilizes single‐cell RNA‐seq transcriptomes, advanced imaging, and electrophysiology to examine universal and cell‐type‐specific aging signatures of vestibular hair cells. The study shows that impaired hair bundle function is a key driver of age‐related vestibular dysfunction.
Samadhi Kulasooriya, Huizhan Liu, Sarath Vijayakumar, Celia Bloom, Zhenhang Xu, Shu Tu, Benjamin J. Borgmeier, Mi Zhou, Litao Tao, Bechara Kachar, David Z. He   +10 more
wiley   +1 more source

Advances in Gastric Cancer Research: Insights Into Carcinogenesis, the Tumor Microenvironment, Metastasis, and Factors Influencing Prognosis

Annals of Gastroenterological Surgery, EarlyView.
ABSTRACT Background The Department of Gastroenterological Surgery at Kumamoto University has maintained a commitment to integrating cutting‐edge clinical practice with fundamental research, particularly concerning malignant diseases of the digestive tract.
Hideo Baba, Takatsugu Ishimoto, Yoshifumi Baba, Hiromitsu Hayashi, Masaaki Iwatsuki   +4 more
wiley   +1 more source

Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway

Nature Communications, 2016
Autophagy requires transport of autophagosomes to the perinuclear region. Here, the authors show that ORP1L localizes to autophagosomes and mediates formation of ER contact sites that prevent autophagosome transport and fusion with endocytic vesicles ...
Ruud H. Wijdeven, Hans Janssen, Leila Nahidiazar, Lennert Janssen, Kees Jalink, Ilana Berlin, Jacques Neefjes   +6 more
doaj   +1 more source

Vimentin inhibition modulates mTORC1 activity and fusion of autophagosomes and lysosomes.

, 2019
A) i) HEK293 cells were untreated or treated with DMSO, 1μM WFA or 2μM WFA for 6 h. Protein lysates were immunoblotted for endogenous rpS6, phosphorylated rpS6 (p-rpS6 (S235/236)) and tubulin.
Graham P. Wright (6277880), Craig Stevens (4331830), Jack Satsangi (259264), Olga Biskou (6277868), Peter G. Barlow (341308), Sadie Kemp (6277877), Kirsty M. Hooper (6277874), Victor Casanova (6277871)   +7 more
core   +1 more source

Unraveling a Diagnostic Enigma: A TECPR2 Case Solved Through Multi‐Omic Genomics

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT TECPR2 is a key regulator of autophagy, encoded by the TECPR2 gene. Pathogenic variants in this gene have been linked to a rare hereditary sensory and autonomic neuropathy with intellectual disability (HSAN9). We report a teenage female with a syndromic intellectual disability disorder associated with neuromuscular abnormalities.
Teresa Zhao, Andrew P. Fennell, Tanavi Sharma, Katrina M. Bell, Monique Dunstan, Sebastian Lunke, Meagan J. McGrath, Catriona McLean, Undiagnosed Diseases Network (UDN‐Aus), Alison Yeung, Anna Hackett, Anne Baxter, Ansley Morrish, Ashil Davawala, Azure Hermes, Ben Kamien, Ben Lundie, Carolyn Ellaway, Carolyn Shalhoub, Cas Simons, Cassandra Gray, Cathryn Poulton, Chloe Cunningham, Chris Barnett, Chris Richmond, Christopher Richards, Daniel MacArthur, Daniel Pavlic, Daniella Hock, Daniz Kooshavar, David Amor, David Mowat, Edward Formaini, Elaine Zhang, Ella Wilkins, Ella Zurita, Ellenore Martin, Elly Lynch, Emma Krzesinski, Emma Palmer, Esther Pierini, Evanthia O. Madelli, Francisco Santos Gonzalez, Gareth Baynam, Gunjan Garg, Hamish Scott, Hannah Thomson, Himanshu Goel, Ilias Goranitis, Ira Deveson, Isabella Pfundt, Jacqui Russell, Janine Smith, Jason Pinner, Julia Broadbent, Julie McGaughran, Karin Kassahn, Katherine Lewis, Kaustuv Bhattacharya, Kirsten Boggs, Kirsty West, Kristi Jones, Laura Wedd, Lauren Dreyer, Leah Frajman, Leanne Baxter, Lilian Downie, Lily Loughman, Lisa Bristowe, Lisa Ewans, Louise Cilento, Lucy Kevin, Lyndal Douglas, Madeleine Harris, Maie Walsh, Manisha Chauhan, Margit Shah, Martin Delatycki, Mathew Wallis, Matthew Hunter, Megan Ball, Megan Higgins, Meutia Kumaheri, Michael Fahey, Mike Field, Mohammadreza Hajjari, Natalie Stewart, Natalie Tan, Natasha Brown, Nicole Van Bergen, Noelia Nunez‐Martinez, Oliver Heath, Rachel Austin, Rani Sachdev, Rebecca Macintosh, Rebecca Vink, Rocio Rius, Ruvi Samarasekera, Ryan Pysar, Sandra Cooper, Sarah Casauria, Sarah Collinson, Sarah Jelenich, Sarah Josephi‐Taylor, Sarah Sandaradura, Sean Massey, Shannon Leblanc, Shuxiang Goh, Simon Bodek, Simon Sadedin, Simran Kaur, Smitha Kumble, Sue White, Suzanne Sallevelt, Tegan Stait, Tiffany Boughtwood, Tim Sikora, Timo Lassmann, Tiong Tan, Tracy Dudding, David R. Thorburn, David A. Stroud, John Christodoulou   +122 more
wiley   +1 more source

Transmission electron microscopic (TEM) analysis of autophagosomes.

, 2019
U2OS-shBRUCE cells with BRUCE depleted (+DOX) or proficient (-DOX) were processed for TEM analysis (A and B, see Materials for details) and analyzed for the number of autosomes and autolysosomes under TEM showing autophagosomes (black open triangles) and
Thomas J. Herzog (6704024), Salim S. El-Amouri (5220368), Chunmin Ge (838812), Lixiao Che (573566), Dao Pan (3105903), Qi-En Wang (283608), Chunying Du (838813), Xingyuan Yang (2283877)   +7 more
core   +1 more source