Results 171 to 180 of about 47,090 (208)

A novel ELISPOT assay to enhance detection of antigen-specific T cells employing antigen-presenting cells expressing vector-driven human B7-1

Journal of Immunological Methods, 2003
The ELISPOT assay is used to detect T-cell responses in patients enrolled in vaccine clinical trials; it is a relatively sensitive assay that can be performed without in vitro stimulation (IVS) of PBMC. However, this assay may be limited in some cases because of a weak signal 1 (as is the case for tumor-associated antigens [TAA]), or by a limited ...
Philip M, Arlen, James L, Gulley, Claudia, Palena, John, Marshall, Jeffrey, Schlom, Kwong Yok, Tsang   +5 more
openaire   +2 more sources

Importance of B7-1-Expressing Host Antigen-Presenting Cells for the Eradication of B7-2 Transfected P815 Tumor Cells

The Journal of Immunology, 1998
Abstract We have previously shown that B7-2 (CD86)-transfected P815 tumor cells elicit tumor-eradicating immunity that leads to the regression of the B7-2+ P815 tumor after transient growth in normal DBA/2 mice. Here, we show that both the B7-2 and B7-1 (CD80) molecules contribute to the eradication of B7-2+ P815 tumors as treatment ...
R N, La Motte, A H, Sharpe, J A, Bluestone, M B, Mokyr   +3 more
openaire   +2 more sources

Differential up-regulation of the B7-1 and B7-2 costimulatory molecules after Ig receptor engagement by antigen.

The Journal of Immunology, 1994
Abstract Ag-pulsed B cells are potent APCs, in part, because of the ability of the Ig receptor to mediate rapid and specific Ag uptake. However, it is also known that full T cell activation requires signals delivered by costimulatory molecules, which naive B cells seem to lack. This study examines the effect Ig receptor engagement has
D J, Lenschow, A I, Sperling, M P, Cooke, G, Freeman, L, Rhee, D C, Decker, G, Gray, L M, Nadler, C C, Goodnow, J A, Bluestone   +9 more
openaire   +2 more sources

IL-12 Reverses Established Antigen-Specific Tolerance of Contact Sensitivity by Affecting Costimulatory Molecules B7-1 (CD80) and B7-2 (CD86)

The Journal of Immunology, 1998
Abstract Cutaneous painting with reactive haptens induces contact sensitivity (CS) responses that are in vivo examples of T cell immunity. In contrast, high dose i.v. administration of the hapten can induce tolerance. We investigated the effect of IL-12 on reversal of this tolerance and attempted to determine in vitro the mechanism of
H, Ushio, R F, Tsuji, M, Szczepanik, K, Kawamoto, H, Matsuda, P W, Askenase   +5 more
openaire   +2 more sources

Interferon‐γ and interleukin‐10 inhibit antigen presentation by Langerhans cells for T helper type 1 cells by suppressing their CD80 (B7‐1) expression

European Journal of Immunology, 1996
AbstractCD80(B7‐1) and CD86(B7‐2) co‐stimulatory molecules have been reported to activate Th1/Th2 development pathways differentially. It is well known that Langerhans cells (LC), potent antigen‐presenting dendritic cells in the epidermis, express several co‐stimulatory molecules and that this expression is modulated by several cytokines.
H, Ozawa, S, Aiba, , Nakagawa, H, Tagami
openaire   +2 more sources

B7-1 expression by a non-antigen presenting cell-derived tumor.

Cancer research, 1995
The existence of a naturally occurring immunosurveillance against neoplastic cells is controversial. A difficulty with this concept is that tumor-specific antigen-reactive T cells would not be expected to become activated after encountering tumor cells, since T cells that bind to antigen in the absence of the costimulation provided by antigen ...
S J, Antonia, T, Muñoz-Antonia, G, Soldevila, J, Miller, R A, Flavell   +4 more
openaire   +1 more source

B7-1 antigen expression in tumor cells from cancerous human tissues.

Analytical and quantitative cytology and histology, 2000
To explore the expression of B7-1 antigen (Ag), the most important costimulatory molecule for T-lymphocyte activation, on tumor cells from cancerous human tissues.The study group consisted of 82 biopsies taken from a variety of cancerous human tissues.
L, Si, H, Si, Y, Chen, Y, Sun, Y, Wing
openaire   +1 more source

Complete amino acid sequence of a papain-solubilized human histocompatibility antigen, HLA-B7. 1. Isolation and amino acid composition of fragments and of tryptic and chymotryptic peptides

Biochemistry, 1979
As a part of the overall strategy for determining the complete covalent structure of the papain-solubilized portion of the heavy chain of the human histocompatibility antigen HLA-B7, the protein was dissected into various fragments by a combination of partial acid hydrolysis and cyanogen bromide cleavage.
J A, López de Castro, H T, Orr, R J, Robb, T G, Kostyk, D L, Mann, J L, Strominger   +5 more
openaire   +2 more sources

CD80 (B7-1) and CD86 (B7-2) antigens on house dust mite–specific T cells in atopic disease function through T-T cell interactions

Journal of Allergy and Clinical Immunology, 1999
CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. Recent studies have demonstrated that these costimulatory molecules are also expressed on activated T cells. However, the functional role of CD80 and CD86 expressed on allergen-specific T cells in atopic diseases has not yet been clarified.We sought to ...
M, Nakada, K, Nishizaki, T, Yoshino, M, Okano, T, Yamamoto, Y, Masuda, N, Ohta, T, Akagi   +7 more
openaire   +2 more sources

Molecular cloning of the cDNAs encoding the feline B‐lymphocyte activation antigen B7–1 (CD80) and B7–2 (CD86) homologues which interact with human CTLA4‐Ig

European Journal of Immunogenetics, 2000
AbstractWe cloned the cDNAs encoding the feline homologues of B‐lymphocyte activation antigens B7–1 (CD80) and B7–2 (CD86). We expressed recombinant feline CD80 and CD86 molecules by the baculovirus expression system, and demonstrated their binding ability to human CTLA4‐murine immunoglobulin fusion protein.
Y, Nishimura, M, Shimojima, T, Miyazawa, E, Sato, K, Nakamura, Y, Izumiya, Y, Ikeda, T, Mikami, E, Takahashi   +8 more
openaire   +2 more sources