Results 11 to 20 of about 799 (124)

Identification of MscS as a Key L‐Glutamate Exporter in Bacillus methanolicus [PDF]

Microbial Biotechnology
Small‐conductance mechanosensitive channels (MscS) are established l‐glutamate exporters in industrially relevant bacteria, yet their role in the methylotrophic bacterium Bacillus methanolicus, a promising platform for sustainable methanol‐based l ...
Luciana Fernandes Brito, Davide Luciano, Marta Irla, David Virant, Gaston Courtade, Trygve Brautaset   +5 more
doaj   +5 more sources

Development of Bacillus methanolicus methanol dehydrogenase with improved formaldehyde reduction activity [PDF]

Scientific Reports, 2018
Methanol dehydrogenase (MDH), an NAD+-dependent oxidoreductase, reversibly converts formaldehyde to methanol. This activity is a key step for both toxic formaldehyde elimination and methanol production in bacterial methylotrophy.
Jiyeun Yi, Jinhyuk Lee, Bong Hyun Sung, Du-Kyeong Kang, GyuTae Lim, Jung-Hoon Bae, Seung-Goo Lee, Sun Chang Kim, Jung-Hoon Sohn   +8 more
doaj   +5 more sources

Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference [PDF]

Frontiers in Microbiology, 2021
The Gram-positive Bacillus methanolicus shows plasmid-dependent methylotrophy. This facultative ribulose monophosphate (RuMP) cycle methylotroph possesses two fructose bisphosphate aldolases (FBA) with distinct kinetic properties.
Kerstin Schultenkämper, Desirée D. Gütle, Marina Gil López, Laura B. Keller, Lin Zhang, Oliver Einsle, Jean-Pierre Jacquot, Volker F. Wendisch   +7 more
doaj   +2 more sources

Development of a markerless tool for targeted chromosome modification in the thermophilic and methylotrophic bacterium Bacillus methanolicus [PDF]

Microbial Cell Factories
Background Bacillus methanolicus is a promising candidate to become an industrial workhorse for methanol-based bioproduction due to its methylotrophy. However, genetic toolbox for this biotechnologically relevant thermophilic bacterium is still limited ...
Marta Irla, Luciana Fernandes Brito, Jesper Langlo, Carsten Wohlers, Leonie Benninghaus, Chantel Heid, Volker F. Wendisch, Jochen Schmid, Trygve Brautaset   +8 more
doaj   +2 more sources

Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus. [PDF]

BMC Microbiol, 2014
Markert B, Stolzenberger J, Brautaset T, Wendisch VF. Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus. BMC Microbiology. 2014;14(1):
Markert B, Stolzenberger J, Brautaset T, Wendisch VF.   +3 more
europepmc   +4 more sources

Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production [PDF]

Frontiers in Microbiology, 2016
Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter and a putative mannitol activator
Marta Irla, Tonje M. B. Heggeset, Ingemar Nærdal, Lidia Paul, Tone Haugen, Simone B. Le, Trygve Brautaset, Trygve Brautaset, Volker F. Wendisch   +8 more
doaj   +2 more sources

Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol [PDF]

Bioengineering, 2023
The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2.
Jiaying Wang, Zhiqiang Chen, Xiaogui Deng, Qianqian Yuan, Hongwu Ma   +4 more
doaj   +2 more sources

Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. [PDF]

PLoS ONE, 2013
Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B.
Anne Krog, Tonje M B Heggeset, Jonas E N Müller, Christiane E Kupper, Olha Schneider, Julia A Vorholt, Trond E Ellingsen, Trygve Brautaset   +7 more
doaj   +2 more sources

Bacillus methanolicus sp. nov., a New Species of Thermotolerant, Methanol-Utilizing, Endospore-Forming Bacteria [PDF]

International Journal of Systematic Bacteriology, 1992
The generic position of 14 strains of gram-positive bacteria able to use methanol as a growth substrate was determined. All are obligately aerobic, thermotolerant organisms that are able to grow at temperatures of 35 to 60°C.
Arfman, Nico, Bulygina, Eugenia S., Chumakov, Konstantin M., Dijkhuizen, Lubbert, Govorukhina, Natalya I., Kirchhof, Gudrun, Ludwig, Wolfgang, Schleifer, Karl-Heinz, Sharp, Richard J., Trotsenko, Yuri A., White, Duncan   +10 more
core   +8 more sources

Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs [PDF]

Synthetic and Systems Biotechnology, 2023
Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals, owing to its high degree of reduction, abundance, and low price.
Arslan Sarwar, Eun Yeol Lee
doaj   +2 more sources