Cloning, Expression, and Sequence Analysis of the Bacillus methanolicus C1 Methanol Dehydrogenase Gene [PDF]
Journal of Bacteriology, 1992 The gene (mdh) coding for methanol dehydrogenase (MDH) of thermotolerant, methylotroph Bacillus methanolicus C1 has been cloned and sequenced.
Arfman, Nico +3 more
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Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy [PDF]
Nature CommunicationsSynthetic methylotrophy offers opportunities for sustainable chemical and biofuel production. While recently established methylotrophic E. coli can grow on methanol, undesirable formate accumulation occurs during growth and bioproduction.
Philipp Keller +10 more
doaj +2 more sources
Methanol-based production of putrescine and spermidine in Bacillus methanolicus [PDF]
, 2023 Verdiskapende forbindelser som aminosyrer produseres ofte gjennom mikrobiell produksjon ved bruk av glukose som råstoff. Dagens glukosepriser stiger, og i tillegg skaper bruk av glukose uønsket konkurranse i mat- og råstoff industries.
Arampu, Adshaha
core +2 more sources
Evolutionary engineering of methylotrophic E. coli enables fast growth on methanol [PDF]
Nature CommunicationsAs methanol can be derived from either CO2 or methane, methanol economy can play an important role in combating climate change. In this scenario, rapid utilization of methanol by an industrial microorganism is the first and crucial step for efficient ...
Liang-Yu Nieh +7 more
doaj +2 more sources
Transcriptome analysis of thermophilic methylotrophic Bacillus methanolicus MGA3 using RNA-sequencing provides detailed insights into its previously uncharted transcriptional landscape. [PDF]
BMC Genomics, 2015 Irla M +5 more
europepmc +2 more sources
Exploring the Potential of Bacillus methanolicus for Expansion of C1 Substrate Range [PDF]
, 2023 Bruken av den metylotrofe og termofile bakterien B. methanolicus MGA3 som en vert for syntetisk metanotrofi representerer en tiltalende mulighet til å bruke billig metangass som karbonkilde i bioteknologisk produksjon. Konverteringen av metan til metanol
Lund, Simen Jervell
core +2 more sources
Identification and characterization of a novel formaldehyde dehydrogenase in Bacillus subtilis. [PDF]
Appl Environ MicrobiolFormaldehyde is a known toxic compound, and functional formaldehyde detoxification is crucial for the survival of all living cells. Such detoxification systems are of particular importance for methylotrophic microorganisms that rely on formaldehyde as a ...
Klein VJ +5 more
europepmc +3 more sources
Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2. [PDF]
ACS Synth Biol, 2019 Synthetic methylotrophy, the modification of organisms such as E. coli to grow on methanol, is a longstanding goal of metabolic engineering and synthetic biology. The poor kinetic properties of NAD-dependent methanol dehydrogenase, the first enzyme in most methanol assimilation pathways, limit pathway flux and present a formidable challenge to ...
Roth TB +3 more
europepmc +5 more sources
Developing a Riboswitch-Mediated Regulatory System for Metabolic Flux Control in Thermophilic Bacillus methanolicus. [PDF]
Int J Mol Sci, 2021 Genome-wide transcriptomic data obtained in RNA-seq experiments can serve as a reliable source for identification of novel regulatory elements such as riboswitches and promoters. Riboswitches are parts of the 5′ untranslated region of mRNA molecules that can specifically bind various metabolites and control gene expression.
Irla M, Hakvåg S, Brautaset T.
europepmc +5 more sources
Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus. [PDF]
Microb Cell Fact, 2016 The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria into an efficient microbial cell factory.
Carnicer M +4 more
europepmc +7 more sources