Results 31 to 40 of about 799 (124)

Efficient cell factories for the production of N-methylated amino acids and for methanol-based amino acid production. [PDF]

Microb Biotechnol, 2022
Methanol is a reduced form of carbon dioxide that can be utilized by methylotrophic microorganisms. In this review, we review on how this feedstock, that does not have competing uses in food or feed, serves amino acid fermentation processes. Summary The growing world needs commodity amino acids such as L‐glutamate and L‐lysine for use as food and feed,
Irla M, Wendisch VF.
europepmc   +2 more sources

Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains. [PDF]

Microb Biotechnol, 2019
Naerdal, Ingemar, Pfeifenschneider, Johannes, Brautaset, Trygve, Wendisch, Volker F.   +3 more
europepmc   +5 more sources

Plasmid-dependent methylotrophy in thermotolerant Bacillus methanolicus. [PDF]

J Bacteriol, 2004
ABSTRACT Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50°C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph.
Brautaset T, Jakobsen M ØM, Flickinger MC, Valla S, Ellingsen TE.   +4 more
europepmc   +4 more sources

Methanol-based acetoin production by genetically engineeredBacillus methanolicus [PDF]

Green Chemistry, 2020
Establishment of sustainable technology for methanol-based production of acetoin by metabolically engineeredBacillus methanolicus.
Eivind B. Drejer, Dennis Tin Chat Chan, Carsten Haupka, Volker F. Wendisch, Trygve Brautaset, Marta Irla   +5 more
openaire   +3 more sources

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Microbial Biotechnology, Volume 16, Issue 2, Page 218-237, February 2023., 2023
Carbon dioxide recycling is a compelling need and microbial carbon dioxide fixation in value‐added compounds is a valuable opportunity. Fermentation of CO2 gas streams using acetogenic bacteria is consolidating as a key biotechnology to move toward a cyclic carbon economy.
Luca Ricci, Arne Seifert, Sebastien Bernacchi, Debora Fino, Candido Fabrizio Pirri, Angela Re   +5 more
wiley   +1 more source

Design, construction and optimization of formaldehyde growth biosensors with broad application in biotechnology. [PDF]

Microb Biotechnol
In this study, we engineered Escherichia coli to function as cellular biosensors capable of detecting a broad range of formaldehyde concentrations. By using natural and promiscuous formaldehyde assimilation enzymes, we created three distinct growth biosensor strains that rely on formaldehyde for cell growth, demonstrating sensitivity from approximately
Schann K, Bakker J, Boinot M, Kuschel P, He H, Nattermann M, Paczia N, Erb T, Bar-Even A, Wenk S.   +9 more
europepmc   +2 more sources

Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph. [PDF]

Appl Environ Microbiol, 1997
We report the fist genetic transformation system, shuttle vectors, and integrative vectors for the thermotolerant, methylotrophic bacterium Bacillus methanolicus. By using a polyethylene glycol-mediated transformation procedure, we have successfully transformed B. methanolicus with both integrative and multicopy plasmids.
Cue D, Lam H, Dillingham RL, Hanson RS, Flickinger MC.   +4 more
europepmc   +4 more sources

Development and application of CRISPR‐based genetic tools in Bacillus species and Bacillus phages

Journal of Applied Microbiology, Volume 133, Issue 4, Page 2280-2298, October 2022., 2022
Abstract Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been developed into a precise and efficient genome editing tool. Since its discovery as an adaptive immune system in prokaryotes, it has been applied in many different research fields including biotechnology and medical sciences.
Yafeng Song, Siqi He, Anita Jopkiewicz, Rita Setroikromo, Ronald van Merkerk, Wim J. Quax   +5 more
wiley   +1 more source

Structural and catalytic properties of the peroxygenase P450 enzyme CYP152K6 from Bacillus methanolicus.

J Inorg Biochem, 2018
The CYP152 family of cytochrome P450 enzymes (P450s or CYPs) are bacterial peroxygenases that use hydrogen peroxide to drive hydroxylation and decarboxylation of fatty acid substrates. We have expressed and purified a novel CYP152 family member - CYP152K6 from the methylotroph Bacillus methanolicus MGA3.
Girvan HM, Poddar H, McLean KJ, Nelson DR, Hollywood KA, Levy CW, Leys D, Munro AW.   +7 more
europepmc   +4 more sources

Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains. [PDF]

Microb Biotechnol, 2015
Summary Methanol is regarded as an attractive substrate for biotechnological production of value‐added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium B acillus
Naerdal I, Pfeifenschneider J, Brautaset T, Wendisch VF.   +3 more
europepmc   +6 more sources