Results 51 to 60 of about 799 (124)

Development of a Novel Assay for Synthesis and Hydrolysis of Sedoheptulose 1,7-bisphosphate (SBP) in vitro by Combinations of Purified Fructose 1,6-bisphosphate aldolases (FBA) Proteins and Fructose 1,6-bisphosphatases (FBPase) Proteins from Bacillus methanolicus MGA3

open access: yesBio-Protocol, 2014
Bacillus methanolicus (B. methanolicus) is a Gram-positive, thermotolerant, and facultative methylotrophic bacterium that can use the one-carbon (C1) compound methanol as a source of carbon and energy (Schendel et al., 1990; Arfman et al., 1997; Arfman ...
Jessica Stolzenberger   +4 more
doaj   +1 more source

From Brown Seaweed to a Sustainable Microbial Feedstock for the Production of Riboflavin [PDF]

open access: yes, 2022
The increasing global demand for food and energy production encourages the development of new production strategies focused on sustainability. Often, microbial bioprocesses rely on food or feed competitive feedstocks; hence, there is a trending need for ...
Brautaset, Trygve   +3 more
core   +1 more source

Production of Propene from n‐Butanol: A Three‐Step Cascade Utilizing the Cytochrome P450 Fatty Acid Decarboxylase OleTJE

open access: yesChemBioChem, Volume 21, Issue 22, Page 3273-3281, November 16, 2020., 2020
Propelled to propene: Propene is produced in a three‐step enzymatic cascade from n‐butanol via butanal and butyric acid, with the last step being oxidative decarboxylation catalyzed by OleTJE. Variation of the supply of redox donors demonstrates the peroxygenase nature of OleTJE.
Daniel Bauer   +2 more
wiley   +1 more source

Bacillus methanolicus MGA3 as host for methanol-based production of L-tryptophan-derived specialty compounds violacein, indole and tryptamine [PDF]

open access: yes, 2022
Interesse i bruk av en-karbon forbindelser, som metanol, som energikilde for produksjon av bioteknologiske relevante stoffer har økt de siste årene.
Korneliussen, Daniel
core  

Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus. [PDF]

open access: yesAppl Environ Microbiol, 1996
We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.
Cue D, Lam H, Hanson RS, Flickinger MC.
europepmc   +4 more sources

An “energy‐auxotroph” Escherichia coli provides an in vivo platform for assessing NADH regeneration systems

open access: yesBiotechnology and Bioengineering, Volume 117, Issue 11, Page 3422-3434, November 2020., 2020
Wenk, Schann and coworkers have constructed an Escherichia coli strain deleted in dihydrolipoyl dehydrogenase, which participates in the pyruvate and 2‐ketoglutarate dehydrogenase complexes. When cultivated on acetate, this strain is auxotrophic to NADH and ATP, and thus provides a useful platform for comparing and optimizing enzymatic systems for ...
Sebastian Wenk   +6 more
wiley   +1 more source

Syngas and Methanol‐Based Biorefinery Concepts

open access: yesChemie Ingenieur Technik, Volume 92, Issue 11, Page 1680-1699, November 2020., 2020
The use of syngas, which can be derived from CO2 or exhaust gases of steel mills, is gaining interest for the synthesis of added‐value products. In this review, the state of the art and technological challenges emerging from the use of this gaseous feedstock are described, as well as the possibility of converting syngas into methanol and its subsequent
Cláudio J. R. Frazão, Thomas Walther
wiley   +1 more source

CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5 [PDF]

open access: yes, 2020
Fernandes de Brito L, Schultenkämper K, Passaglia L, Wendisch VF. CRISPR interference-based gene repression in the plant growth promoter Paenibacillus sonchi genomovar Riograndensis SBR5. Applied Microbiology and Biotechnology .
Fernandes de Brito, Luciana   +3 more
core   +2 more sources

Microbial Engineering for Production of N‐Functionalized Amino Acids and Amines

open access: yesBiotechnology Journal, Volume 15, Issue 7, July 2020., 2020
Microbial production of N‐functionalized amines and amino acids is reviewed in terms of recent advances and applications and the prospects of developing new fermentation processes. N‐functionalizing enzymes and strain engineering are focused here. Abstract N‐functionalized amines play important roles in nature and occur, for example, in the antibiotic ...
Melanie Mindt   +3 more
wiley   +1 more source

Impact of CRISPR interference on strain development in biotechnology

open access: yesBiotechnology and Applied Biochemistry, Volume 67, Issue 1, Page 7-21, January/February 2020., 2020
CRISPR interference drives metabolic engineering in biotechnology. Abstract Genetic perturbation systems are of great interest to redirect metabolic fluxes for value‐added production, as well as genetic screening for the development of new drugs, or to identify new targets for biotechnological applications. Here, we review CRISPR interference (CRISPRi),
Kerstin Schultenkämper   +2 more
wiley   +1 more source

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