Results 91 to 100 of about 298,183 (327)

Coupling Nitrogenous Organic Wastewater Treatment and Biorefinery via N‐Cycling Bacterium

Advanced Science, EarlyView.
The vast reservoir of nitrogenous organic pollutants in industrial wastewaters can serve as untapped carbon‐nitrogen resources. This study employs the newly discovered Paracoccus sp. ZQW‐1 as a versatile chassis to simultaneously achieve N‐methylpyrrolidone (NMP) wastewater depuration and polyhydroxybutyrate (PHB) synthesis.
Ziqian Wang, Chunyu Du, Guanyu Zheng, Dahu Ding   +3 more
wiley   +1 more source

Site‐Selective Ligand Selection by Mutational Profiling for Covalent RNA Targeting

Angewandte Chemie, EarlyView.
Using a mutational profiling approach an RNA‐targeted covalent probe was developed that is highly selective for the FMN riboswitch. The covalency was further supported by mass spectrometry experiments. The covalent probe selectively modifies the riboswitch at low micromolar concentrations within 10 min and remains functional when applied to an entire ...
Phillip Yesley, Georgia Poulladofonou, Danny Incarnato, Willem A. Velema   +3 more
wiley   +2 more sources

Calcium signalling in Bacillus subtilis

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1998
Few systematic studies have been devoted to investigating the role of Ca2+ as an intracellular messenger in prokaryotes. Here we report an investigation on the potential involvement of Ca2+ in signalling in Bacillus subtilis, a Gram-positive bacterium. Using aequorin, it is shown that B. subtilis cells tightly regulate intracellular Ca2+ levels.
Herbaud, Marie-Laure, Guiseppi, Annick, Denizot, François, Haiech, Jacques, Kilhoffer, Marie-Claude   +4 more
openaire   +2 more sources

CRISPR in MOF Formulation with Enhanced Stability, Activity, and Altered PAM Specificity for Broad‐Spectrum Diagnosis of Bacterial Sepsis

Advanced Science, EarlyView.
CRISPR‐FLEXMO (CRISPR with flexible PAM in MOF encapsulation) integrates a PAM‐relaxed Cas12a K607R enzyme with a manganese‐coordinated MOF for broad‐spectrum diagnosis of bacterial sepsis. The MOF enhances activity and preserves functionality under heat, solvent, and chaotropic stress, supporting long‐term room‐temperature storage.
Tathagata Pal, Zilong Liu, Meera G. Nair, Juhong Chen   +3 more
wiley   +1 more source

Optimized In‐Solution and Gas‐Phase Chemistry Enables High‐Efficiency Interactome Mapping by DSBSO‐Based Cross‐Linking Mass Spectrometry

Angewandte Chemie, EarlyView.
We developed a rapid, high‐throughput XL–MS workflow using the enrichable azide‐A‐DSBSO cross‐linker and StageTip‐based SCX fractionation. It drastically reduces preparation time and enhances sensitivity, providing a scalable platform for high‐throughput profiling of protein interaction networks.
Pin‐Lian Jiang, Ying Zhu, Jiaxin Cai, Cong Wang, Mei Wu, Ke Pu, Fan Liu   +6 more
wiley   +2 more sources

An In Silico Approach for Comparative Characterization of Imidazolonepropionase from Agrobacterium fabrum & Bacillus subtilis: An Imperative Enzyme for Histidine Degradation

Biology and Life Sciences Forum
Introduction: The third step in histidine degradation is catalysed by imidazolonepropionase. It catalyses the conversion of 4-imidazolone-5-propionic acid to produce N-formimino-L-glutamic acid by hydrolyzing the carbon-nitrogen bonds. The histidine is a
Ishita Biswas, Trishanjan Biswas, Debanjan Mitra   +2 more
doaj   +1 more source

Surface charge and hydrodynamic coefficient measurements of {\it Bacillus subtilis} spore by Optical Tweezers

, 2014
In this work we report on the simultaneous measurement of the hydrodynamic coefficient and the electric charge of single {\it Bacillus subtilis} spores.
Isticato, Rachele, Pesce, Giuseppe, Ricca, Ezio, Rusciano, Giulia, Sasso, Antonio, Sirec, Teja   +5 more
core   +1 more source

Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 [PDF]

, 2005
We report here the sequencing and analysis of the genome of the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901. This species is a model for studies of hydrogenogens, which are diverse bacteria and archaea that grow anaerobically ...
A Scott Durkin, Daniel H Haft, Frank T Robb, Igor B Zhulin, James F Kolonay, Jonathan A Eisen, Juan M Gonzalez, Kristine M Jones, Lauren M Brinkac, Luke E Ulrich, Luke J Tallon, Martin Wu, Qinghu Ren, Ramana Madupu, Robert J Dodson, Sean C Daugherty, Steven A Sullivan, William C Nelson   +17 more
core   +7 more sources

A Modular Chemoenzymatic Cascade Simplifies Divergent Synthesis of Natural and Unnatural Benzylisoquinoline Alkaloids

Advanced Science, EarlyView.
A simplified chemoenzymatic platform is designed to integrate an enzyme cascade with subsequent chemical steps, enabling the divergent production of diverse benzylisoquinoline alkaloids (BIAs). Demonstrating remarkable versatility and enantioselectivity, four distinct classes of BIAs are efficiently generated, in which the natural medicine papaverine ...
Huiling Liu, Zhenbo Yuan, Yue Gao, Fei Li, Zhiwei Deng, Yan Zhang, Zhengshan Luo, Changmei Liu, Yijian Rao   +8 more
wiley   +1 more source

The potential of Bacillus strains isolated from the rumen content of dairy cows as natural antibacterial and antioxidant agents for broilers

Journal of the Indonesian Tropical Animal Agriculture, 2018
The study was conducted to investigate the in vitro antibacterial activity and antioxidant-enhancing effect of Bacillus strains isolated from the rumen content of dairy cows. The antibacterial activity of Bacillus strains was assessed against Escherichia
S. Sugiharto, T. Yudiarti, I. Isroli, E. Widiastuti, H.I. Wahyuni, E. Suprijatna   +5 more
doaj   +1 more source